A method for rapidly screening functionality of actin mutants and tagged actins
<p>Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity and functionality of...
Main Authors: | Rommelaere Heidi, Waterschoot Davy, Neirynck Katrien, Vandekerckhove Joël, Ampe Christophe |
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Format: | Article |
Language: | English |
Published: |
BMC
2004-01-01
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Series: | Biological Procedures Online |
Subjects: | |
Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/94/m94.pdf |
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