STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS
Purpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activity of the compounds was determined by the MTT method. Antiviral activity of subst...
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| Format: | Article |
| Language: | English |
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Odessa I. I. Mechnikov National University
2014-03-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
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| Online Access: | http://mbt.onu.edu.ua/article/view/48195 |
| _version_ | 1818880775287734272 |
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| author | Л. О. Білявська О. Ю. Повниця Ю. Г. Шермолович Г. П. Гудзь Н. В. Нестерова |
| author_facet | Л. О. Білявська О. Ю. Повниця Ю. Г. Шермолович Г. П. Гудзь Н. В. Нестерова |
| author_sort | Л. О. Білявська |
| collection | DOAJ |
| description | Purpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activity of the compounds was determined by the MTT method. Antiviral activity of substances was studied using cyto-morphological method with acridine orange dye. The virus-specific intranuclear inclusions were determined. Results. In present study the cytotoxicity of 2N-substituted-4-tosyl-5-polyfluoroalkyl-1,2,3-triazoles for Hep-2 and Hela cell lines were studied. The dependence of cytotoxicity on the compounds in the structure of substituents in the aromatic rings of the molecules was defined. Combination of residues of 3-hlorotetrahydropyran, 3-chloro-dihydrofuran tetrahydrofuran in a glycosidic moiety with trifluoromethyl or perfluoropropyl in a triazole ring were more toxic. Antiviral activity of 3 compounds (G7, G8 and G10) against HAdV5 in vitro was revealed. The effective antiviral concentrations (EC50) for them were 16, 70 and 186 mg/ml, and the selectivity indexes were equal to 91, 8 and 4, respectively. Conclusions. High rates of antiviral activity and low cytotoxicity of G8 compound make it perspective for development antiviral agents. |
| first_indexed | 2024-12-19T14:51:19Z |
| format | Article |
| id | doaj.art-3b45795551634de38b0756783499d040 |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-19T14:51:19Z |
| publishDate | 2014-03-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-3b45795551634de38b0756783499d0402022-12-21T20:16:49ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632014-03-0101(25)192610.18524/2307-4663.2014.1(25).4819548195STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDSЛ. О. Білявська0О. Ю. Повниця1Ю. Г. Шермолович2Г. П. Гудзь3Н. В. Нестерова4Інститут мікробіології і вірусології НАН УкраїниІнститут мікробіології і вірусології НАН УкраїниІнститут органічної хімії НАН УкраїниІнститут органічної хімії НАН УкраїниІнститут мікробіології і вірусології НАН УкраїниPurpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activity of the compounds was determined by the MTT method. Antiviral activity of substances was studied using cyto-morphological method with acridine orange dye. The virus-specific intranuclear inclusions were determined. Results. In present study the cytotoxicity of 2N-substituted-4-tosyl-5-polyfluoroalkyl-1,2,3-triazoles for Hep-2 and Hela cell lines were studied. The dependence of cytotoxicity on the compounds in the structure of substituents in the aromatic rings of the molecules was defined. Combination of residues of 3-hlorotetrahydropyran, 3-chloro-dihydrofuran tetrahydrofuran in a glycosidic moiety with trifluoromethyl or perfluoropropyl in a triazole ring were more toxic. Antiviral activity of 3 compounds (G7, G8 and G10) against HAdV5 in vitro was revealed. The effective antiviral concentrations (EC50) for them were 16, 70 and 186 mg/ml, and the selectivity indexes were equal to 91, 8 and 4, respectively. Conclusions. High rates of antiviral activity and low cytotoxicity of G8 compound make it perspective for development antiviral agents.http://mbt.onu.edu.ua/article/view/48195фторовмісні нуклеозидиаденовірусантивірусна активність |
| spellingShingle | Л. О. Білявська О. Ю. Повниця Ю. Г. Шермолович Г. П. Гудзь Н. В. Нестерова STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS Mìkrobìologìâ ì Bìotehnologìâ фторовмісні нуклеозиди аденовірус антивірусна активність |
| title | STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS |
| title_full | STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS |
| title_fullStr | STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS |
| title_full_unstemmed | STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS |
| title_short | STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS |
| title_sort | study of antiadenoviralactivity of new fluorinecontaining heterocyclic compounds |
| topic | фторовмісні нуклеозиди аденовірус антивірусна активність |
| url | http://mbt.onu.edu.ua/article/view/48195 |
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