Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis
Exosomes carry molecules of great biological and clinical interest, such as miRNAs. The contents of exosomes vary between healthy controls and cancer patients. Therefore, miRNAs and other molecules transported in exosomes are considered a potential source of diagnostic and prognostic biomarkers in c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Non-Coding RNA |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-553X/8/3/33 |
| _version_ | 1797483725091504128 |
|---|---|
| author | Andrés Rincón-Riveros Josefa Antonia Rodríguez Victoria E. Villegas Liliana López-Kleine |
| author_facet | Andrés Rincón-Riveros Josefa Antonia Rodríguez Victoria E. Villegas Liliana López-Kleine |
| author_sort | Andrés Rincón-Riveros |
| collection | DOAJ |
| description | Exosomes carry molecules of great biological and clinical interest, such as miRNAs. The contents of exosomes vary between healthy controls and cancer patients. Therefore, miRNAs and other molecules transported in exosomes are considered a potential source of diagnostic and prognostic biomarkers in cancer. Many miRNAs have been detected in recent years. Consequently, a substantial amount of miRNA-related data comparing patients and healthy individuals is available, which contributes to a better understanding of the initiation, development, malignancy, and metastasis of cancer using non-invasive sampling procedures. However, a re-analysis of available ncRNA data is rare. This study used available data about miRNAs in exosomes comparing healthy individuals and cancer patients to identify possible global changes related to the presence of cancer. A robust transcriptomic analysis identified two common miRNAs (miR-495-3p and miR-543) deregulated in five cancer datasets. They had already been implicated in different cancers but not reported in exosomes circulating in blood. The study also examined their target genes and the implications of these genes for functional processes. |
| first_indexed | 2024-03-09T22:52:07Z |
| format | Article |
| id | doaj.art-3b4ba65af74a49a1b28fc0a7e50bc994 |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-09T22:52:07Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-3b4ba65af74a49a1b28fc0a7e50bc9942023-11-23T18:18:07ZengMDPI AGNon-Coding RNA2311-553X2022-05-01833310.3390/ncrna8030033Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network AnalysisAndrés Rincón-Riveros0Josefa Antonia Rodríguez1Victoria E. Villegas2Liliana López-Kleine3Bioinformatics and Systems Biology Group, Universidad Nacional de Colombia, Bogotá 111221, ColombiaCancer Biology Research Group, Instituto Nacional de Cancerología, Bogotá 111221, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá 111221, ColombiaDepartment of Statistics, Faculty of Science, Universidad Nacional de Colombia, Bogotá 111221, ColombiaExosomes carry molecules of great biological and clinical interest, such as miRNAs. The contents of exosomes vary between healthy controls and cancer patients. Therefore, miRNAs and other molecules transported in exosomes are considered a potential source of diagnostic and prognostic biomarkers in cancer. Many miRNAs have been detected in recent years. Consequently, a substantial amount of miRNA-related data comparing patients and healthy individuals is available, which contributes to a better understanding of the initiation, development, malignancy, and metastasis of cancer using non-invasive sampling procedures. However, a re-analysis of available ncRNA data is rare. This study used available data about miRNAs in exosomes comparing healthy individuals and cancer patients to identify possible global changes related to the presence of cancer. A robust transcriptomic analysis identified two common miRNAs (miR-495-3p and miR-543) deregulated in five cancer datasets. They had already been implicated in different cancers but not reported in exosomes circulating in blood. The study also examined their target genes and the implications of these genes for functional processes.https://www.mdpi.com/2311-553X/8/3/33miRNAextracellular vesiclescancer |
| spellingShingle | Andrés Rincón-Riveros Josefa Antonia Rodríguez Victoria E. Villegas Liliana López-Kleine Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis Non-Coding RNA miRNA extracellular vesicles cancer |
| title | Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis |
| title_full | Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis |
| title_fullStr | Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis |
| title_full_unstemmed | Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis |
| title_short | Identification of Two Exosomal miRNAs in Circulating Blood of Cancer Patients by Using Integrative Transcriptome and Network Analysis |
| title_sort | identification of two exosomal mirnas in circulating blood of cancer patients by using integrative transcriptome and network analysis |
| topic | miRNA extracellular vesicles cancer |
| url | https://www.mdpi.com/2311-553X/8/3/33 |
| work_keys_str_mv | AT andresrinconriveros identificationoftwoexosomalmirnasincirculatingbloodofcancerpatientsbyusingintegrativetranscriptomeandnetworkanalysis AT josefaantoniarodriguez identificationoftwoexosomalmirnasincirculatingbloodofcancerpatientsbyusingintegrativetranscriptomeandnetworkanalysis AT victoriaevillegas identificationoftwoexosomalmirnasincirculatingbloodofcancerpatientsbyusingintegrativetranscriptomeandnetworkanalysis AT lilianalopezkleine identificationoftwoexosomalmirnasincirculatingbloodofcancerpatientsbyusingintegrativetranscriptomeandnetworkanalysis |