Qualitative Analysis of Hydroxyurea
Hydroxyurea (HU) is a cytostatic agent with antineoplastic activity and it has great clinical efficacy in the treatment of sickle cell disease. HU is part of the National Relation of Essential Medicines of Brazil and despite of its clinical importance, only the Portuguese, American and European Phar...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal do Rio Grande do Sul
2020-07-01
|
| Series: | Drug Analytical Research |
| Online Access: | https://seer.ufrgs.br/index.php/produto&producao/ojs/include/index.php/dar/article/view/100682 |
| _version_ | 1797388931282501632 |
|---|---|
| author | Stéfanno Souza Santos Isis Gomes dos Santos Cinara Vanconcelos da Silva Edith C L Cazedey |
| author_facet | Stéfanno Souza Santos Isis Gomes dos Santos Cinara Vanconcelos da Silva Edith C L Cazedey |
| author_sort | Stéfanno Souza Santos |
| collection | DOAJ |
| description | Hydroxyurea (HU) is a cytostatic agent with antineoplastic activity and it has great clinical efficacy in the treatment of sickle cell disease. HU is part of the National Relation of Essential Medicines of Brazil and despite of its clinical importance, only the Portuguese, American and European Pharmacopoeias present monographs for this medicine. This study aimed to develop qualitative methods for identification and verification of purity of HU. For the HU identification, IR and UV spectroscopy were used, as well as TLC. The IR and UV spectra showed compatibility and the maximum absorption at 213 nm. For TLC, various pure or polarity gradient solvents were tested with results satisfactory for a mobile phase containing acetone and ethyl acetate (99:1, v/v). The replacement of the mobile phase generated less chemical waste, while minimizing the risks to the health of the operator, in accordance with the main concept of green analytical chemistry. |
| first_indexed | 2024-03-08T22:48:02Z |
| format | Article |
| id | doaj.art-3b4c9926a36b459b968fcd32730b6c01 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-03-08T22:48:02Z |
| publishDate | 2020-07-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-3b4c9926a36b459b968fcd32730b6c012023-12-16T18:13:38ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162020-07-0141Qualitative Analysis of HydroxyureaStéfanno Souza Santos0Isis Gomes dos Santos1Cinara Vanconcelos da Silva2Edith C L Cazedey3Núcleo de Pesquisa e Análise de Medicamentos (NuPAM), Faculdade de Farmácia, Universidade Federal da BahiaNúcleo de Pesquisa e Análise de Medicamentos (NuPAM), Programa de Pós-Graduação em Farmácia (PPGFAR), Faculdade de Farmácia, Universidade Federal da BahiaNúcleo de Pesquisa e Análise de Medicamentos (NuPAM), Faculdade de Farmácia, Universidade Federal da BahiaNúcleo de Pesquisa e Análise de Medicamentos (NuPAM), Programa de Pós-Graduação em Farmácia (PPGFAR), Faculdade de Farmácia, Universidade Federal da BahiaHydroxyurea (HU) is a cytostatic agent with antineoplastic activity and it has great clinical efficacy in the treatment of sickle cell disease. HU is part of the National Relation of Essential Medicines of Brazil and despite of its clinical importance, only the Portuguese, American and European Pharmacopoeias present monographs for this medicine. This study aimed to develop qualitative methods for identification and verification of purity of HU. For the HU identification, IR and UV spectroscopy were used, as well as TLC. The IR and UV spectra showed compatibility and the maximum absorption at 213 nm. For TLC, various pure or polarity gradient solvents were tested with results satisfactory for a mobile phase containing acetone and ethyl acetate (99:1, v/v). The replacement of the mobile phase generated less chemical waste, while minimizing the risks to the health of the operator, in accordance with the main concept of green analytical chemistry.https://seer.ufrgs.br/index.php/produto&producao/ojs/include/index.php/dar/article/view/100682 |
| spellingShingle | Stéfanno Souza Santos Isis Gomes dos Santos Cinara Vanconcelos da Silva Edith C L Cazedey Qualitative Analysis of Hydroxyurea Drug Analytical Research |
| title | Qualitative Analysis of Hydroxyurea |
| title_full | Qualitative Analysis of Hydroxyurea |
| title_fullStr | Qualitative Analysis of Hydroxyurea |
| title_full_unstemmed | Qualitative Analysis of Hydroxyurea |
| title_short | Qualitative Analysis of Hydroxyurea |
| title_sort | qualitative analysis of hydroxyurea |
| url | https://seer.ufrgs.br/index.php/produto&producao/ojs/include/index.php/dar/article/view/100682 |
| work_keys_str_mv | AT stefannosouzasantos qualitativeanalysisofhydroxyurea AT isisgomesdossantos qualitativeanalysisofhydroxyurea AT cinaravanconcelosdasilva qualitativeanalysisofhydroxyurea AT edithclcazedey qualitativeanalysisofhydroxyurea |