Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions

Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions

Use of dual sgRNAs is a common CRISPR/Cas9-based strategy for the creation of genetic deletions. The ease of screening combined with a rather high rate of success makes this approach a reliable genome engineering procedure. Recently, a number of studies using CRISPR/Cas9 have revealed unwanted large...

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Bibliographic Details
Main Authors: Joseph Blayney, Evangeline M. Foster, Marta Jagielowicz, Mira Kreuzer, Matteo Morotti, Katharina Reglinski, Julie Huiyuan Xiao, Philip Hublitz
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Methods and Protocols
Subjects:
CRISPR/Cas9
dual sgRNA
genomic knock-out
NHEJ
PCR screen
inverted re-insertion
Online Access:https://www.mdpi.com/2409-9279/3/3/53

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