SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway
Abstract Background Ovarian cancer is the leading cause of death in gynecological cancer. Cancer stem cells (CSCs) contribute to the occurrence, progression and resistance. Small nucleolar RNAs (SnoRNAs), a class of small molecule non-coding RNA, involve in the cancer cell stemness and tumorigenesis...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-019-2005-1 |
| _version_ | 1818218494825594880 |
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| author | Wenjing Zhu Jumin Niu Miao He Liwen Zhang Xuemei Lv Fangxiao Liu Longyang Jiang Jing Zhang Zhaojin Yu Lin Zhao Jia Bi Yuanyuan Yan Qian Wei Hong Huo Yue Fan Yuzong Chen Jian Ding Minjie Wei |
| author_facet | Wenjing Zhu Jumin Niu Miao He Liwen Zhang Xuemei Lv Fangxiao Liu Longyang Jiang Jing Zhang Zhaojin Yu Lin Zhao Jia Bi Yuanyuan Yan Qian Wei Hong Huo Yue Fan Yuzong Chen Jian Ding Minjie Wei |
| author_sort | Wenjing Zhu |
| collection | DOAJ |
| description | Abstract Background Ovarian cancer is the leading cause of death in gynecological cancer. Cancer stem cells (CSCs) contribute to the occurrence, progression and resistance. Small nucleolar RNAs (SnoRNAs), a class of small molecule non-coding RNA, involve in the cancer cell stemness and tumorigenesis. Methods In this study, we screened out SNORNAs related to ovarian patient’s prognosis by analyzing the data of 379 cases of ovarian cancer patients in the TCGA database, and analyzed the difference of SNORNAs expression between OVCAR-3 (OV) sphere-forming (OS) cells and OV cells. After overexpression or knockdown SNORD89, the expression of Nanog, CD44, and CD133 was measured by qRT-PCR or flow cytometry analysis in OV, CAOV-3 (CA) and OS cells, respectively. CCK-8 assays, plate clone formation assay and soft agar colony formation assay were carried out to evaluate the changes of cell proliferation and self-renewal ability. Scratch migration assay and trans-well invasion analysis were used for assessing the changes of migration and invasion ability. Results High expression of SNORD89 indicates the poor prognosis of ovarian cancer patients and was associated with patients’ age, therapy outcome. SNORD89 highly expressed in ovarian cancer stem cells. The overexpression of SNORD89 resulted in the increased stemness markers, S phase cell cycle, cell proliferation, invasion and migration ability in OV and CA cells. Conversely, these phenomena were reversed after SNORD89 silencing in OS cells. Further, we found that SNORD89 could upregulate c-Myc and Notch1 expression in mRNA and protein levels. SNORD89 deteriorates the prognosis of ovarian cancer patients by regulating Notch1-c-Myc pathway to promote cell stemness and acts as an oncogene in ovarian tumorigenesis. Consequently, SNORD89 can be a novel prognostic biomarker and therapeutic target for ovarian cancer. |
| first_indexed | 2024-12-12T07:24:40Z |
| format | Article |
| id | doaj.art-3b531d4ee0bd43ac89148cd939998f94 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-12T07:24:40Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-3b531d4ee0bd43ac89148cd939998f942022-12-22T00:33:12ZengBMCJournal of Translational Medicine1479-58762019-08-0117111510.1186/s12967-019-2005-1SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathwayWenjing Zhu0Jumin Niu1Miao He2Liwen Zhang3Xuemei Lv4Fangxiao Liu5Longyang Jiang6Jing Zhang7Zhaojin Yu8Lin Zhao9Jia Bi10Yuanyuan Yan11Qian Wei12Hong Huo13Yue Fan14Yuzong Chen15Jian Ding16Minjie Wei17Department of Pharmacology, School of Pharmacy, China Medical UniversityShenyang Women’s and Children’s HospitalDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmaceutics, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityBioinformatics and Drug Design Group, Department of Pharmacy, National University of SingaporeDepartment of Pharmacology, School of Pharmacy, China Medical UniversityDepartment of Pharmacology, School of Pharmacy, China Medical UniversityAbstract Background Ovarian cancer is the leading cause of death in gynecological cancer. Cancer stem cells (CSCs) contribute to the occurrence, progression and resistance. Small nucleolar RNAs (SnoRNAs), a class of small molecule non-coding RNA, involve in the cancer cell stemness and tumorigenesis. Methods In this study, we screened out SNORNAs related to ovarian patient’s prognosis by analyzing the data of 379 cases of ovarian cancer patients in the TCGA database, and analyzed the difference of SNORNAs expression between OVCAR-3 (OV) sphere-forming (OS) cells and OV cells. After overexpression or knockdown SNORD89, the expression of Nanog, CD44, and CD133 was measured by qRT-PCR or flow cytometry analysis in OV, CAOV-3 (CA) and OS cells, respectively. CCK-8 assays, plate clone formation assay and soft agar colony formation assay were carried out to evaluate the changes of cell proliferation and self-renewal ability. Scratch migration assay and trans-well invasion analysis were used for assessing the changes of migration and invasion ability. Results High expression of SNORD89 indicates the poor prognosis of ovarian cancer patients and was associated with patients’ age, therapy outcome. SNORD89 highly expressed in ovarian cancer stem cells. The overexpression of SNORD89 resulted in the increased stemness markers, S phase cell cycle, cell proliferation, invasion and migration ability in OV and CA cells. Conversely, these phenomena were reversed after SNORD89 silencing in OS cells. Further, we found that SNORD89 could upregulate c-Myc and Notch1 expression in mRNA and protein levels. SNORD89 deteriorates the prognosis of ovarian cancer patients by regulating Notch1-c-Myc pathway to promote cell stemness and acts as an oncogene in ovarian tumorigenesis. Consequently, SNORD89 can be a novel prognostic biomarker and therapeutic target for ovarian cancer.http://link.springer.com/article/10.1186/s12967-019-2005-1Ovarian cancer stem cellsTCGASNORNAsSNORD89 |
| spellingShingle | Wenjing Zhu Jumin Niu Miao He Liwen Zhang Xuemei Lv Fangxiao Liu Longyang Jiang Jing Zhang Zhaojin Yu Lin Zhao Jia Bi Yuanyuan Yan Qian Wei Hong Huo Yue Fan Yuzong Chen Jian Ding Minjie Wei SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway Journal of Translational Medicine Ovarian cancer stem cells TCGA SNORNAs SNORD89 |
| title | SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway |
| title_full | SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway |
| title_fullStr | SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway |
| title_full_unstemmed | SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway |
| title_short | SNORD89 promotes stemness phenotype of ovarian cancer cells by regulating Notch1-c-Myc pathway |
| title_sort | snord89 promotes stemness phenotype of ovarian cancer cells by regulating notch1 c myc pathway |
| topic | Ovarian cancer stem cells TCGA SNORNAs SNORD89 |
| url | http://link.springer.com/article/10.1186/s12967-019-2005-1 |
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