<i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i>
As with other environmental stresses, cold stress limits plant growth, geographical distribution, and agricultural productivity. <i>CBF/DREB</i> (<i>CRT</i>-binding factors/<i>DRE</i>-binding proteins) regulate tolerance to cold/freezing stress across plant specie...
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MDPI AG
2021-10-01
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| author | Chia-Ling Wu Lee-Fong Lin Hsiao-Chun Hsu Li-Fen Huang Chung-Der Hsiao Ming-Lun Chou |
| author_facet | Chia-Ling Wu Lee-Fong Lin Hsiao-Chun Hsu Li-Fen Huang Chung-Der Hsiao Ming-Lun Chou |
| author_sort | Chia-Ling Wu |
| collection | DOAJ |
| description | As with other environmental stresses, cold stress limits plant growth, geographical distribution, and agricultural productivity. <i>CBF/DREB</i> (<i>CRT</i>-binding factors/<i>DRE</i>-binding proteins) regulate tolerance to cold/freezing stress across plant species. ICE (inducer of <i>CBF</i> expression) is regarded as the upstream inducer of <i>CBF</i> expression and plays a crucial role as a main regulator of cold acclimation. Snow lotus (<i>Saussurea involucrat</i><i>a</i>) is a well-known traditional Chinese herb. This herb is known to have greater tolerance to cold/freezing stress compared to other plants. According to transcriptome datasets, two putative <i>ICE</i> homologous genes, <i>SiICE1</i> and <i>SiICE2</i>, were identified in snow lotus. The predicted <i>SiICE1</i> cDNA contains an ORF of 1506 bp, encoding a protein of 501 amino acids, whereas <i>SiICE2</i> cDNA has an ORF of 1482 bp, coding for a protein of 493 amino acids. Sequence alignment and structure analysis show SiICE1 and SiICE2 possess a S-rich motif at the N-terminal region, while the conserved ZIP-bHLH domain and ACT domain are at the C-terminus. Both <i>SiICE1</i> and <i>SiICE2</i> transcripts were cold-inducible. Subcellular localization and yeast one-hybrid assays revealed that SiICE1 and SiICE2 are transcriptional regulators. Overexpression of <i>SiICE1</i> (<i>35S::SiICE1</i>) and <i>SiICE2</i> (<i>35S::SiICE2</i>) in transgenic <i>Arabidopsis</i> increased the cold tolerance. In addition, the expression patterns of downstream stress-related genes, <i>CBF1</i>, <i>CBF2</i>, <i>CBF3</i>, <i>COR15A</i>, <i>COR47</i>, and <i>KIN1</i>, were up-regulated when compared to the wild type. These results thus provide evidence that SiICE1 and SiICE2 function in cold acclimation and this cold/freezing tolerance may be regulated through a CBF-controlling pathway. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:58:41Z |
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| spelling | doaj.art-3b536e1bbbfa4bf9b1f07741cb4e9ce62023-11-22T16:16:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122191085010.3390/ijms221910850<i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i>Chia-Ling Wu0Lee-Fong Lin1Hsiao-Chun Hsu2Li-Fen Huang3Chung-Der Hsiao4Ming-Lun Chou5Department of Life Sciences, Tzu Chi University, Hualien 97004, TaiwanDepartment of Life Sciences, Tzu Chi University, Hualien 97004, TaiwanThe Society of Wilderness, Taipei 10073, TaiwanGraduate School of Biotechnology and Bioengineering, Yuan Ze University, Zhongli, Taoyuan 32003, TaiwanDepartment of Bioscience Technology, Chung Yuan Christian University, Chung-Li 320314, TaiwanDepartment of Life Sciences, Tzu Chi University, Hualien 97004, TaiwanAs with other environmental stresses, cold stress limits plant growth, geographical distribution, and agricultural productivity. <i>CBF/DREB</i> (<i>CRT</i>-binding factors/<i>DRE</i>-binding proteins) regulate tolerance to cold/freezing stress across plant species. ICE (inducer of <i>CBF</i> expression) is regarded as the upstream inducer of <i>CBF</i> expression and plays a crucial role as a main regulator of cold acclimation. Snow lotus (<i>Saussurea involucrat</i><i>a</i>) is a well-known traditional Chinese herb. This herb is known to have greater tolerance to cold/freezing stress compared to other plants. According to transcriptome datasets, two putative <i>ICE</i> homologous genes, <i>SiICE1</i> and <i>SiICE2</i>, were identified in snow lotus. The predicted <i>SiICE1</i> cDNA contains an ORF of 1506 bp, encoding a protein of 501 amino acids, whereas <i>SiICE2</i> cDNA has an ORF of 1482 bp, coding for a protein of 493 amino acids. Sequence alignment and structure analysis show SiICE1 and SiICE2 possess a S-rich motif at the N-terminal region, while the conserved ZIP-bHLH domain and ACT domain are at the C-terminus. Both <i>SiICE1</i> and <i>SiICE2</i> transcripts were cold-inducible. Subcellular localization and yeast one-hybrid assays revealed that SiICE1 and SiICE2 are transcriptional regulators. Overexpression of <i>SiICE1</i> (<i>35S::SiICE1</i>) and <i>SiICE2</i> (<i>35S::SiICE2</i>) in transgenic <i>Arabidopsis</i> increased the cold tolerance. In addition, the expression patterns of downstream stress-related genes, <i>CBF1</i>, <i>CBF2</i>, <i>CBF3</i>, <i>COR15A</i>, <i>COR47</i>, and <i>KIN1</i>, were up-regulated when compared to the wild type. These results thus provide evidence that SiICE1 and SiICE2 function in cold acclimation and this cold/freezing tolerance may be regulated through a CBF-controlling pathway.https://www.mdpi.com/1422-0067/22/19/10850ICE orthologuescold stress tolerance<i>CBF</i> expressionsnow lotus |
| spellingShingle | Chia-Ling Wu Lee-Fong Lin Hsiao-Chun Hsu Li-Fen Huang Chung-Der Hsiao Ming-Lun Chou <i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> International Journal of Molecular Sciences ICE orthologues cold stress tolerance <i>CBF</i> expression snow lotus |
| title | <i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_full | <i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_fullStr | <i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | <i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_short | <i>Saussurea involucrata</i> (Snow Lotus) <i>ICE1</i> and <i>ICE2</i> Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_sort | i saussurea involucrata i snow lotus i ice1 i and i ice2 i orthologues involved in regulating cold stress tolerance in transgenic i arabidopsis i |
| topic | ICE orthologues cold stress tolerance <i>CBF</i> expression snow lotus |
| url | https://www.mdpi.com/1422-0067/22/19/10850 |
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