PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i>
Being an invasive plant, <i>Polygonum cuspidatum</i> is highly resilient and can survive in unfavorable environments for long periods; however, its molecular mechanisms associated with such environmental resistance are largely unknown. In this study, a WRKY transcription factor (TF) gene...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Guowei Wang Xiaowei Wang Hongping Ma Haili Fan Fan Lin Jianhui Chen Tuanyao Chai Hong Wang |
| author_facet | Guowei Wang Xiaowei Wang Hongping Ma Haili Fan Fan Lin Jianhui Chen Tuanyao Chai Hong Wang |
| author_sort | Guowei Wang |
| collection | DOAJ |
| description | Being an invasive plant, <i>Polygonum cuspidatum</i> is highly resilient and can survive in unfavorable environments for long periods; however, its molecular mechanisms associated with such environmental resistance are largely unknown. In this study, a WRKY transcription factor (TF) gene, <i>PcWRKY11</i>, was identified from <i>P. cuspidatum</i> by analyzing methyl jasmonate (MeJA)-treated transcriptome data. It showed a high degree of homology with WRKY11 from <i>Arabidopsis thaliana</i>, containing a WRKY domain and a zinc finger structure and II-d WRKY characteristic domains of HARF, a calmodulin-binding domain (C-motif), and a putative nuclear localization signal (NLS) through sequence alignment and functional element mining. qPCR analysis showed that the expression of PcWRKY11 can be induced by NaCl, osmotic stress, and UV-C. In this study, we also found that overexpression of <i>PcWRKY11</i> in <i>A</i><i>. thaliana</i> could significantly increase salt tolerance. To explore its possible molecular mechanism, further investigations showed that compared with the wild type (WT), under salt stress, the transgenic plants showed a lower malondialdehyde (MDA) content, higher expression of <i>ascorbate peroxidase (APX</i>) and <i>superoxide dismutase</i> (<i>SOD</i>), and higher enzyme activity of peroxidase <i>(</i>POD), superoxide dismutase (SOD), and catalase (CAT). Moreover, the transgenic plants also showed higher expression of Δ<sup>1</sup>-pyrroline-5-carboxylate synthase (<i>AtP5CS</i>), and higher contents of proline and soluble sugar. Taken together, these results indicate that <i>PcWRKY11</i> may have a positive role in plants’ adaptation to salinity conditions by reducing reactive oxygen species (ROS) levels and increasing osmosis substance synthesis. |
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| spelling | doaj.art-3b55552d36b64d70977f35e7061d4a6b2023-12-01T21:04:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238435710.3390/ijms23084357PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i>Guowei Wang0Xiaowei Wang1Hongping Ma2Haili Fan3Fan Lin4Jianhui Chen5Tuanyao Chai6Hong Wang7College of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, ChinaBeing an invasive plant, <i>Polygonum cuspidatum</i> is highly resilient and can survive in unfavorable environments for long periods; however, its molecular mechanisms associated with such environmental resistance are largely unknown. In this study, a WRKY transcription factor (TF) gene, <i>PcWRKY11</i>, was identified from <i>P. cuspidatum</i> by analyzing methyl jasmonate (MeJA)-treated transcriptome data. It showed a high degree of homology with WRKY11 from <i>Arabidopsis thaliana</i>, containing a WRKY domain and a zinc finger structure and II-d WRKY characteristic domains of HARF, a calmodulin-binding domain (C-motif), and a putative nuclear localization signal (NLS) through sequence alignment and functional element mining. qPCR analysis showed that the expression of PcWRKY11 can be induced by NaCl, osmotic stress, and UV-C. In this study, we also found that overexpression of <i>PcWRKY11</i> in <i>A</i><i>. thaliana</i> could significantly increase salt tolerance. To explore its possible molecular mechanism, further investigations showed that compared with the wild type (WT), under salt stress, the transgenic plants showed a lower malondialdehyde (MDA) content, higher expression of <i>ascorbate peroxidase (APX</i>) and <i>superoxide dismutase</i> (<i>SOD</i>), and higher enzyme activity of peroxidase <i>(</i>POD), superoxide dismutase (SOD), and catalase (CAT). Moreover, the transgenic plants also showed higher expression of Δ<sup>1</sup>-pyrroline-5-carboxylate synthase (<i>AtP5CS</i>), and higher contents of proline and soluble sugar. Taken together, these results indicate that <i>PcWRKY11</i> may have a positive role in plants’ adaptation to salinity conditions by reducing reactive oxygen species (ROS) levels and increasing osmosis substance synthesis.https://www.mdpi.com/1422-0067/23/8/4357<i>Polygonum cuspidatum</i>PcWRKY11salt stressROStransgenic <i>Arabidopsis thaliana</i> |
| spellingShingle | Guowei Wang Xiaowei Wang Hongping Ma Haili Fan Fan Lin Jianhui Chen Tuanyao Chai Hong Wang PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i> International Journal of Molecular Sciences <i>Polygonum cuspidatum</i> PcWRKY11 salt stress ROS transgenic <i>Arabidopsis thaliana</i> |
| title | PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_full | PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_fullStr | PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_short | PcWRKY11, an II-d WRKY Transcription Factor from <i>Polygonum cuspidatum</i>, Enhances Salt Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_sort | pcwrky11 an ii d wrky transcription factor from i polygonum cuspidatum i enhances salt tolerance in transgenic i arabidopsis thaliana i |
| topic | <i>Polygonum cuspidatum</i> PcWRKY11 salt stress ROS transgenic <i>Arabidopsis thaliana</i> |
| url | https://www.mdpi.com/1422-0067/23/8/4357 |
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