Phosphatase activity in salt-ponds of the Bay of Cadiz
Acid and alkaline phosphatase activities (E.C.3.1.3.2 and E.C.3.1.3.1, respectively) were measured in seawater and sediments from different sites of a salt-pond of the Bay of Cádiz. The profiles of enzymatic activity relative to depth were also analysed. Initially, the optimum conditions (pH, tempe...
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| Format: | Article |
| Language: | English |
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Universidad Autónoma de Baja California
2004-03-01
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| Series: | Ciencias Marinas |
| Subjects: | |
| Online Access: | https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/332 |
| _version_ | 1827330854654836736 |
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| author | MD Frutos J Blasco A Gómez-Parra |
| author_facet | MD Frutos J Blasco A Gómez-Parra |
| author_sort | MD Frutos |
| collection | DOAJ |
| description |
Acid and alkaline phosphatase activities (E.C.3.1.3.2 and E.C.3.1.3.1, respectively) were measured in seawater and sediments from different sites of a salt-pond of the Bay of Cádiz. The profiles of enzymatic activity relative to depth were also analysed. Initially, the optimum conditions (pH, temperature, substrate concentration and amount of sample) for determining enzymatic activities were studied. The apparent activation energies for alkaline phosphatase activity in seawater and sediments were 53.8 and 48.6 kJ mol–1, respectively. The Km and Vmax were 10.6 mM and 89 ∞g of 4-nitrophenol g–1 h–1 and 8.42 mM and 145 ∞g of 4-nitrophenol L–1 h–1 for sediments and seawater, respectively. Both types of enzymatic activity reached high values in seawater and sediments; in the confined areas of the salt-pond the values were around 100 ∞g of 4-nitrophenol L–1 h–1 for seawater and higher for sediments. In general, clear seasonal evolutions for both types of enzymatic activity were found in the seawater, with maxima at the end of summer and minima in winter. No seasonal pattern was evident in the sediment. Stratification of phosphorus regeneration was observed in the sediment column but there was no stratification at depths greater than 15 cm.
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| first_indexed | 2024-03-07T16:24:24Z |
| format | Article |
| id | doaj.art-3b5fedaf58244cea816e63295a00235d |
| institution | Directory Open Access Journal |
| issn | 0185-3880 2395-9053 |
| language | English |
| last_indexed | 2024-03-07T16:24:24Z |
| publishDate | 2004-03-01 |
| publisher | Universidad Autónoma de Baja California |
| record_format | Article |
| series | Ciencias Marinas |
| spelling | doaj.art-3b5fedaf58244cea816e63295a00235d2024-03-03T18:37:46ZengUniversidad Autónoma de Baja CaliforniaCiencias Marinas0185-38802395-90532004-03-0130310.7773/cm.v30i3.332Phosphatase activity in salt-ponds of the Bay of CadizMD Frutos0J Blasco1A Gómez-Parra2Universidad de CádizInstituto de Ciencias Marinas de AndalucíaUniversidad de Cádiz Acid and alkaline phosphatase activities (E.C.3.1.3.2 and E.C.3.1.3.1, respectively) were measured in seawater and sediments from different sites of a salt-pond of the Bay of Cádiz. The profiles of enzymatic activity relative to depth were also analysed. Initially, the optimum conditions (pH, temperature, substrate concentration and amount of sample) for determining enzymatic activities were studied. The apparent activation energies for alkaline phosphatase activity in seawater and sediments were 53.8 and 48.6 kJ mol–1, respectively. The Km and Vmax were 10.6 mM and 89 ∞g of 4-nitrophenol g–1 h–1 and 8.42 mM and 145 ∞g of 4-nitrophenol L–1 h–1 for sediments and seawater, respectively. Both types of enzymatic activity reached high values in seawater and sediments; in the confined areas of the salt-pond the values were around 100 ∞g of 4-nitrophenol L–1 h–1 for seawater and higher for sediments. In general, clear seasonal evolutions for both types of enzymatic activity were found in the seawater, with maxima at the end of summer and minima in winter. No seasonal pattern was evident in the sediment. Stratification of phosphorus regeneration was observed in the sediment column but there was no stratification at depths greater than 15 cm. https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/332phosphatasesseawatersedimentsalt-pondsBay of Cádiz |
| spellingShingle | MD Frutos J Blasco A Gómez-Parra Phosphatase activity in salt-ponds of the Bay of Cadiz Ciencias Marinas phosphatases seawater sediment salt-ponds Bay of Cádiz |
| title | Phosphatase activity in salt-ponds of the Bay of Cadiz |
| title_full | Phosphatase activity in salt-ponds of the Bay of Cadiz |
| title_fullStr | Phosphatase activity in salt-ponds of the Bay of Cadiz |
| title_full_unstemmed | Phosphatase activity in salt-ponds of the Bay of Cadiz |
| title_short | Phosphatase activity in salt-ponds of the Bay of Cadiz |
| title_sort | phosphatase activity in salt ponds of the bay of cadiz |
| topic | phosphatases seawater sediment salt-ponds Bay of Cádiz |
| url | https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/332 |
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