Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System
This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from gener...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/14/1/27 |
| _version_ | 1797344472651005952 |
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| author | Hyunsoo Seo Young-Su Jeong Jaekyung Bae Kibong Choi Moon-Hyeong Seo |
| author_facet | Hyunsoo Seo Young-Su Jeong Jaekyung Bae Kibong Choi Moon-Hyeong Seo |
| author_sort | Hyunsoo Seo |
| collection | DOAJ |
| description | This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, and simultaneous signals are then converted into photon signals and categorized based on predetermined criteria. A reliable biological particle generator was required to validate the performance of the system. This study explores the use of an M13 bacteriophage as a virus simulant of biological agents and employs a customized inkjet aerosol generator to produce M13 bacteriophage aerosols of a specific size by controlling the concentration of M13. We confirmed that micro-sized, narrowly dispersed M13 aerosols were efficiently generated. Additionally, we confirmed the performance of this real-time handheld bioaerosol monitoring system by detecting viruses. |
| first_indexed | 2024-03-08T11:03:07Z |
| format | Article |
| id | doaj.art-3b621f02158248f099a8b649169c8f0b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-08T11:03:07Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-3b621f02158248f099a8b649169c8f0b2024-01-26T15:23:08ZengMDPI AGBiosensors2079-63742024-01-011412710.3390/bios14010027Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring SystemHyunsoo Seo0Young-Su Jeong1Jaekyung Bae2Kibong Choi3Moon-Hyeong Seo4Chem-Bio Technology Center, Advanced Defense Science and Technology Research Institute, Agency for Defense Development, Daejeon 34186, Republic of KoreaChem-Bio Technology Center, Advanced Defense Science and Technology Research Institute, Agency for Defense Development, Daejeon 34186, Republic of KoreaChem-Bio Technology Center, Advanced Defense Science and Technology Research Institute, Agency for Defense Development, Daejeon 34186, Republic of KoreaChem-Bio Technology Center, Advanced Defense Science and Technology Research Institute, Agency for Defense Development, Daejeon 34186, Republic of KoreaNatural Product Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaThis study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, and simultaneous signals are then converted into photon signals and categorized based on predetermined criteria. A reliable biological particle generator was required to validate the performance of the system. This study explores the use of an M13 bacteriophage as a virus simulant of biological agents and employs a customized inkjet aerosol generator to produce M13 bacteriophage aerosols of a specific size by controlling the concentration of M13. We confirmed that micro-sized, narrowly dispersed M13 aerosols were efficiently generated. Additionally, we confirmed the performance of this real-time handheld bioaerosol monitoring system by detecting viruses.https://www.mdpi.com/2079-6374/14/1/27biological weaponsbio-aerosolvirus aerosolM13 bacteriophageinkjet aerosol generator |
| spellingShingle | Hyunsoo Seo Young-Su Jeong Jaekyung Bae Kibong Choi Moon-Hyeong Seo Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System Biosensors biological weapons bio-aerosol virus aerosol M13 bacteriophage inkjet aerosol generator |
| title | Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System |
| title_full | Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System |
| title_fullStr | Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System |
| title_full_unstemmed | Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System |
| title_short | Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System |
| title_sort | detection of micrometer sized virus aerosols by using a real time bioaerosol monitoring system |
| topic | biological weapons bio-aerosol virus aerosol M13 bacteriophage inkjet aerosol generator |
| url | https://www.mdpi.com/2079-6374/14/1/27 |
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