Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression
One of the causative agents of pokkah boeng disease (PBD), which affects sugarcane crops globally, is the fungus <i>Fusarium sacchari</i>. These fungal infections reduce sugar quality and yield, resulting in severe economic losses. Effector proteins play important roles in the interactio...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/1/59 |
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| author | Zhen Huang Huixue Li Yuming Zhou Yixue Bao Zhenzhen Duan Caixia Wang Charles A. Powell Baoshan Chen Muqing Zhang Wei Yao |
| author_facet | Zhen Huang Huixue Li Yuming Zhou Yixue Bao Zhenzhen Duan Caixia Wang Charles A. Powell Baoshan Chen Muqing Zhang Wei Yao |
| author_sort | Zhen Huang |
| collection | DOAJ |
| description | One of the causative agents of pokkah boeng disease (PBD), which affects sugarcane crops globally, is the fungus <i>Fusarium sacchari</i>. These fungal infections reduce sugar quality and yield, resulting in severe economic losses. Effector proteins play important roles in the interactions between pathogenic fungi and plants. Here, we used bioinformatic prediction approaches to identify 316 candidate secreted effector proteins (CSEPs) in the complete genome of <i>F. sacchari</i>. In total, 95 CSEPs contained known conserved structures, representing 40 superfamilies and 18 domains, while an additional 91 CSEPs contained seven known motifs. Of the 130 CSEPs containing no known domains or motifs, 14 contained one of four novel motifs. A heterogeneous expression system in <i>Nicotiana benthamiana</i> was used to investigate the functions of 163 CSEPs. Seven CSEPs suppressed BAX-triggered programmed cell death in <i>N. benthamiana</i>, while four caused cell death in <i>N. benthamiana</i>. The expression profiles of these eleven CSEPs during <i>F. sacchari</i> infection suggested that they may be involved in sugarcane-<i>F. sacchari</i> interaction. Our results establish a basis for further studies of the role of effector molecules in pathogen–sugarcane interactions, and provide a framework for future predictions of pathogen effector molecules. |
| first_indexed | 2024-03-10T01:10:06Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T01:10:06Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-3b64288d4e734834a7effb088436d6162023-11-23T14:18:21ZengMDPI AGJournal of Fungi2309-608X2022-01-01815910.3390/jof8010059Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous ExpressionZhen Huang0Huixue Li1Yuming Zhou2Yixue Bao3Zhenzhen Duan4Caixia Wang5Charles A. Powell6Baoshan Chen7Muqing Zhang8Wei Yao9State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaIRREC-IFAS, University of Florida, Fort Pierce, FL 34945, USAState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaState Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning 530005, ChinaOne of the causative agents of pokkah boeng disease (PBD), which affects sugarcane crops globally, is the fungus <i>Fusarium sacchari</i>. These fungal infections reduce sugar quality and yield, resulting in severe economic losses. Effector proteins play important roles in the interactions between pathogenic fungi and plants. Here, we used bioinformatic prediction approaches to identify 316 candidate secreted effector proteins (CSEPs) in the complete genome of <i>F. sacchari</i>. In total, 95 CSEPs contained known conserved structures, representing 40 superfamilies and 18 domains, while an additional 91 CSEPs contained seven known motifs. Of the 130 CSEPs containing no known domains or motifs, 14 contained one of four novel motifs. A heterogeneous expression system in <i>Nicotiana benthamiana</i> was used to investigate the functions of 163 CSEPs. Seven CSEPs suppressed BAX-triggered programmed cell death in <i>N. benthamiana</i>, while four caused cell death in <i>N. benthamiana</i>. The expression profiles of these eleven CSEPs during <i>F. sacchari</i> infection suggested that they may be involved in sugarcane-<i>F. sacchari</i> interaction. Our results establish a basis for further studies of the role of effector molecules in pathogen–sugarcane interactions, and provide a framework for future predictions of pathogen effector molecules.https://www.mdpi.com/2309-608X/8/1/59sugarcane<i>Fusarium sacchari</i>effector proteinsPCDqRT-PCR |
| spellingShingle | Zhen Huang Huixue Li Yuming Zhou Yixue Bao Zhenzhen Duan Caixia Wang Charles A. Powell Baoshan Chen Muqing Zhang Wei Yao Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression Journal of Fungi sugarcane <i>Fusarium sacchari</i> effector proteins PCD qRT-PCR |
| title | Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression |
| title_full | Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression |
| title_fullStr | Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression |
| title_full_unstemmed | Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression |
| title_short | Predication of the Effector Proteins Secreted by <i>Fusarium sacchari</i> Using Genomic Analysis and Heterogenous Expression |
| title_sort | predication of the effector proteins secreted by i fusarium sacchari i using genomic analysis and heterogenous expression |
| topic | sugarcane <i>Fusarium sacchari</i> effector proteins PCD qRT-PCR |
| url | https://www.mdpi.com/2309-608X/8/1/59 |
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