Protective Effect of Silybin in Rats Liver Toxicity
Silybin is a flavonoid extracted from the herb Armurariu (Silybum marianum) and has the potential efficacy in the treatment of liver disease. The aims of this study were to investigate the effect of alcohol and CCl4 on liver histology and the capacity of silybin to ameliorate the hepatotoxicity. Thi...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1357 |
| _version_ | 1797215752304984064 |
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| author | Roxana Popescu Doina Verdes Marioara Nicoleta Filimon Laura Smaranda Gotia |
| author_facet | Roxana Popescu Doina Verdes Marioara Nicoleta Filimon Laura Smaranda Gotia |
| author_sort | Roxana Popescu |
| collection | DOAJ |
| description | Silybin is a flavonoid extracted from the herb Armurariu (Silybum marianum) and has the potential efficacy in the treatment of liver disease. The aims of this study were to investigate the effect of alcohol and CCl4 on liver histology and the capacity of silybin to ameliorate the hepatotoxicity. Thirty adult male Wistar rats were used in the study. Liver toxicity was induced by dietary alcohol administration and CCl4 intra-peritoneal injection. The protective effect of silibin was investigated by co-administration of silybin with these toxic agents. Hepatocellular and extracellular matrix integrity was determined by histopathological and immunohistochemical study. Hematoxylin-Eosin and trichrome stains sections were studied in each case. For immunohistochemistry we used monoclonal anti-collagen IV primary antibody. Light microscopic evaluation of liver tissues shows that control and silibin treated groups has normal liver structure. In the toxicity groups, HE and trichromic staining showed hepatocellular necrosis, inflammatory infiltrate and proliferating collagen fibers. Immunoexpression of collagen IV was variable. In the control group, we found negative expression. Collagen IV displays positive immunoreaction in hepatotoxicity groups, at the level of the areas rich in inflammatory infiltrate and with degenerative aspect. After this study, we can conclude that silybin, in rats, has protective effects. |
| first_indexed | 2024-04-24T11:35:03Z |
| format | Article |
| id | doaj.art-3b650d90b51943bebcca9bb74eaf8f47 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:35:03Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-3b650d90b51943bebcca9bb74eaf8f472024-04-10T05:42:35ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-014315065061357Protective Effect of Silybin in Rats Liver ToxicityRoxana Popescu0Doina Verdes1Marioara Nicoleta Filimon2Laura Smaranda Gotia3University of Medicine and Pharmacy “Victor Babes” Timisoara, Department of Cellular and Molecular Biology, 300041, 2 Eftimie Murgu Street, RomaniaUniversity of Medicine and Pharmacy “Victor Babes” Timisoara, Department of Cellular and Molecular Biology, 300041, 2 Eftimie Murgu Street, RomaniaWest University of Timisoara, Faculty of Chemistry-Biology-Geography, Department of Biology, 300115, 16 Pestalozz Stree,t RomaniaUniversity of Medicine and Pharmacy “Victor Babes” Timisoara, Department of Physiology, 300041, 2 Eftimie Murgu Street, RomaniaSilybin is a flavonoid extracted from the herb Armurariu (Silybum marianum) and has the potential efficacy in the treatment of liver disease. The aims of this study were to investigate the effect of alcohol and CCl4 on liver histology and the capacity of silybin to ameliorate the hepatotoxicity. Thirty adult male Wistar rats were used in the study. Liver toxicity was induced by dietary alcohol administration and CCl4 intra-peritoneal injection. The protective effect of silibin was investigated by co-administration of silybin with these toxic agents. Hepatocellular and extracellular matrix integrity was determined by histopathological and immunohistochemical study. Hematoxylin-Eosin and trichrome stains sections were studied in each case. For immunohistochemistry we used monoclonal anti-collagen IV primary antibody. Light microscopic evaluation of liver tissues shows that control and silibin treated groups has normal liver structure. In the toxicity groups, HE and trichromic staining showed hepatocellular necrosis, inflammatory infiltrate and proliferating collagen fibers. Immunoexpression of collagen IV was variable. In the control group, we found negative expression. Collagen IV displays positive immunoreaction in hepatotoxicity groups, at the level of the areas rich in inflammatory infiltrate and with degenerative aspect. After this study, we can conclude that silybin, in rats, has protective effects.https://spasb.ro/index.php/public_html/article/view/1357silybinhepatotoxicityalcoholccl4 |
| spellingShingle | Roxana Popescu Doina Verdes Marioara Nicoleta Filimon Laura Smaranda Gotia Protective Effect of Silybin in Rats Liver Toxicity Scientific Papers Animal Science and Biotechnologies silybin hepatotoxicity alcohol ccl4 |
| title | Protective Effect of Silybin in Rats Liver Toxicity |
| title_full | Protective Effect of Silybin in Rats Liver Toxicity |
| title_fullStr | Protective Effect of Silybin in Rats Liver Toxicity |
| title_full_unstemmed | Protective Effect of Silybin in Rats Liver Toxicity |
| title_short | Protective Effect of Silybin in Rats Liver Toxicity |
| title_sort | protective effect of silybin in rats liver toxicity |
| topic | silybin hepatotoxicity alcohol ccl4 |
| url | https://spasb.ro/index.php/public_html/article/view/1357 |
| work_keys_str_mv | AT roxanapopescu protectiveeffectofsilybininratslivertoxicity AT doinaverdes protectiveeffectofsilybininratslivertoxicity AT marioaranicoletafilimon protectiveeffectofsilybininratslivertoxicity AT laurasmarandagotia protectiveeffectofsilybininratslivertoxicity |