Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp.
The medicinal fungus <i>Ganoderma lucidum</i> contains many bioactive triterpenoids, ganoderic acid A (GAA) being one of the major ones. The present study explored the microbial biotransformation of GAA, isolating 283 strains of soil actinomycetes and determining their abilities to biotr...
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MDPI AG
2018-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/4/4/101 |
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| author | Te-Sheng Chang Horng-Huey Ko Tzi-Yuan Wang Chun-Hsien Lee Jiumn-Yih Wu |
| author_facet | Te-Sheng Chang Horng-Huey Ko Tzi-Yuan Wang Chun-Hsien Lee Jiumn-Yih Wu |
| author_sort | Te-Sheng Chang |
| collection | DOAJ |
| description | The medicinal fungus <i>Ganoderma lucidum</i> contains many bioactive triterpenoids, ganoderic acid A (GAA) being one of the major ones. The present study explored the microbial biotransformation of GAA, isolating 283 strains of soil actinomycetes and determining their abilities to biotransform GAA with ultra-performance liquid chromatography analysis. One positive strain, AI 045, was selected to validate the biotransformation activity. The strain was identified as <i>Streptomyces</i> sp. based on the sequenced 16S rRNA gene. The produced compound obtained from the biotransformation of GAA was purified with the preparative high-performance liquid chromatography method and identified as 3-<i>O</i>-acetyl GAA based on mass and nuclear magnetic resonance spectral data. The present study is the first report that bacteria have the novel ability to biotransform the triterpenoids of fungus <i>G. lucidum</i>. Moreover, the identified 3-<i>O</i>-acetyl GAA is a new triterpenoid product discovered in microbes. |
| first_indexed | 2024-04-12T07:10:21Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-04-12T07:10:21Z |
| publishDate | 2018-12-01 |
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| series | Fermentation |
| spelling | doaj.art-3b665855af4846e08290f4d97bae476d2022-12-22T03:42:39ZengMDPI AGFermentation2311-56372018-12-014410110.3390/fermentation4040101fermentation4040101Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp.Te-Sheng Chang0Horng-Huey Ko1Tzi-Yuan Wang2Chun-Hsien Lee3Jiumn-Yih Wu4Department of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, TaiwanDepartment of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, TaiwanBiodiversity Research Center, Academia Sinica, Taipei 115, TaiwanDepartment of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, TaiwanDepartment of Food Science, National Quemoy University, No. 1, University Road., Jin-Ning Township, Kinmen County 892, TaiwanThe medicinal fungus <i>Ganoderma lucidum</i> contains many bioactive triterpenoids, ganoderic acid A (GAA) being one of the major ones. The present study explored the microbial biotransformation of GAA, isolating 283 strains of soil actinomycetes and determining their abilities to biotransform GAA with ultra-performance liquid chromatography analysis. One positive strain, AI 045, was selected to validate the biotransformation activity. The strain was identified as <i>Streptomyces</i> sp. based on the sequenced 16S rRNA gene. The produced compound obtained from the biotransformation of GAA was purified with the preparative high-performance liquid chromatography method and identified as 3-<i>O</i>-acetyl GAA based on mass and nuclear magnetic resonance spectral data. The present study is the first report that bacteria have the novel ability to biotransform the triterpenoids of fungus <i>G. lucidum</i>. Moreover, the identified 3-<i>O</i>-acetyl GAA is a new triterpenoid product discovered in microbes.https://www.mdpi.com/2311-5637/4/4/101actinomycetesbiotransformation<i>Ganoderma lucidum</i>ganoderic acid A<i>Streptomyces</i> |
| spellingShingle | Te-Sheng Chang Horng-Huey Ko Tzi-Yuan Wang Chun-Hsien Lee Jiumn-Yih Wu Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp. Fermentation actinomycetes biotransformation <i>Ganoderma lucidum</i> ganoderic acid A <i>Streptomyces</i> |
| title | Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp. |
| title_full | Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp. |
| title_fullStr | Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp. |
| title_full_unstemmed | Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp. |
| title_short | Biotransformation of Ganoderic Acid A to 3-<i>O</i>-Acetyl Ganoderic Acid A by Soil-isolated <i>Streptomyces</i> sp. |
| title_sort | biotransformation of ganoderic acid a to 3 i o i acetyl ganoderic acid a by soil isolated i streptomyces i sp |
| topic | actinomycetes biotransformation <i>Ganoderma lucidum</i> ganoderic acid A <i>Streptomyces</i> |
| url | https://www.mdpi.com/2311-5637/4/4/101 |
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