Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy
Hepatitis E virus (HEV) is an important cause of acute viral hepatitis in humans worldwide. The food-borne transmission of HEV appears to be a major route in Europe through the consumption of pork and wild boar meat. HEV epidemiology in wild boars has been investigated mainly in Northern and Central...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/10/2021 |
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| author | Gianfranco La Bella Maria Grazia Basanisi Gaia Nobili Rosa Coppola Annita Maria Damato Adelia Donatiello Gilda Occhiochiuso Antonella Cristina Romano Mariateresa Toce Lucia Palazzo Francesco Pellegrini Angela Fanelli Barbara Di Martino Elisabetta Suffredini Gianvito Lanave Vito Martella Giovanna La Salandra |
| author_facet | Gianfranco La Bella Maria Grazia Basanisi Gaia Nobili Rosa Coppola Annita Maria Damato Adelia Donatiello Gilda Occhiochiuso Antonella Cristina Romano Mariateresa Toce Lucia Palazzo Francesco Pellegrini Angela Fanelli Barbara Di Martino Elisabetta Suffredini Gianvito Lanave Vito Martella Giovanna La Salandra |
| author_sort | Gianfranco La Bella |
| collection | DOAJ |
| description | Hepatitis E virus (HEV) is an important cause of acute viral hepatitis in humans worldwide. The food-borne transmission of HEV appears to be a major route in Europe through the consumption of pork and wild boar meat. HEV epidemiology in wild boars has been investigated mainly in Northern and Central Italian regions, whilst information from Southern Italy is limited. We investigated the occurrence of HEV in wild boar in the Apulia and Basilicata regions (Southern Italy). Thirteen (10.4%) out of one hundred and twenty-five wild boar samples tested positive for HEV using a quantitative reverse transcription PCR. HEV prevalence was 12% in Apulia and 9.3% in Basilicata. Seven samples were genotyped, and different subtypes (c, f, m) of genotype 3 were identified. The complete genome of a 3m strain was determined, and the virus showed the highest nucleotide identity to a human HEV strain identified in France in 2017. These findings demonstrate the substantial circulation of HEV in the wild boar population in Italian Southern regions. Gathering information on the HEV strains circulating in different geographical areas is useful for tracking the origin of HEV outbreaks and assessing the epidemiological role of wild boar as a potential virus reservoir for domestic pigs. |
| first_indexed | 2024-03-10T20:49:43Z |
| format | Article |
| id | doaj.art-3b67c10310cd4bcd8e91e1d715990a32 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:49:43Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3b67c10310cd4bcd8e91e1d715990a322023-11-19T18:27:04ZengMDPI AGViruses1999-49152023-09-011510202110.3390/v15102021Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East ItalyGianfranco La Bella0Maria Grazia Basanisi1Gaia Nobili2Rosa Coppola3Annita Maria Damato4Adelia Donatiello5Gilda Occhiochiuso6Antonella Cristina Romano7Mariateresa Toce8Lucia Palazzo9Francesco Pellegrini10Angela Fanelli11Barbara Di Martino12Elisabetta Suffredini13Gianvito Lanave14Vito Martella15Giovanna La Salandra16Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Bari, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Food Safety Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Bari, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, ItalyHepatitis E virus (HEV) is an important cause of acute viral hepatitis in humans worldwide. The food-borne transmission of HEV appears to be a major route in Europe through the consumption of pork and wild boar meat. HEV epidemiology in wild boars has been investigated mainly in Northern and Central Italian regions, whilst information from Southern Italy is limited. We investigated the occurrence of HEV in wild boar in the Apulia and Basilicata regions (Southern Italy). Thirteen (10.4%) out of one hundred and twenty-five wild boar samples tested positive for HEV using a quantitative reverse transcription PCR. HEV prevalence was 12% in Apulia and 9.3% in Basilicata. Seven samples were genotyped, and different subtypes (c, f, m) of genotype 3 were identified. The complete genome of a 3m strain was determined, and the virus showed the highest nucleotide identity to a human HEV strain identified in France in 2017. These findings demonstrate the substantial circulation of HEV in the wild boar population in Italian Southern regions. Gathering information on the HEV strains circulating in different geographical areas is useful for tracking the origin of HEV outbreaks and assessing the epidemiological role of wild boar as a potential virus reservoir for domestic pigs.https://www.mdpi.com/1999-4915/15/10/2021hepatitis E viruspublic healthwhole genomewild boarzoonosis |
| spellingShingle | Gianfranco La Bella Maria Grazia Basanisi Gaia Nobili Rosa Coppola Annita Maria Damato Adelia Donatiello Gilda Occhiochiuso Antonella Cristina Romano Mariateresa Toce Lucia Palazzo Francesco Pellegrini Angela Fanelli Barbara Di Martino Elisabetta Suffredini Gianvito Lanave Vito Martella Giovanna La Salandra Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy Viruses hepatitis E virus public health whole genome wild boar zoonosis |
| title | Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy |
| title_full | Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy |
| title_fullStr | Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy |
| title_full_unstemmed | Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy |
| title_short | Evidence of Circulation and Phylogenetic Analysis of Hepatitis E Virus (HEV) in Wild Boar in South-East Italy |
| title_sort | evidence of circulation and phylogenetic analysis of hepatitis e virus hev in wild boar in south east italy |
| topic | hepatitis E virus public health whole genome wild boar zoonosis |
| url | https://www.mdpi.com/1999-4915/15/10/2021 |
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