A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn.
Buckwheat seeds contain many kinds of functional compounds that are of benefit to patients with cardiovascular disease. In this research, a water-soluble buckwheat protein was isolated and purified through a DEAE-Sepharose anion exchange column and Sephadex G-75 gel chromatography. The isolated...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2013-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302767Z.pdf |
| _version_ | 1818609194359586816 |
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| author | Zhou Xiaoli Huang Lin Tang Wen Zhou Yiming Wang Qing Li Zongjie |
| author_facet | Zhou Xiaoli Huang Lin Tang Wen Zhou Yiming Wang Qing Li Zongjie |
| author_sort | Zhou Xiaoli |
| collection | DOAJ |
| description | Buckwheat seeds contain many kinds of functional compounds that are of benefit to patients with cardiovascular disease. In this research, a water-soluble buckwheat protein was isolated and purified through a DEAE-Sepharose anion exchange column and Sephadex G-75 gel chromatography. The isolated buckwheat protein fractions exhibited hypocholesterolemic activity in a HepG2 cell model and demonstrated prominent bile acid salt-binding activity in an in vitro assay. The antioxidative activity of protein fractions with hypolipidemic effects was detected in a free radical scavenging experiment. The buckwheat protein fraction with the most obvious hypolipidemic activity and free radical scavenging activity was named as WSBWP. Its molecular weight was estimated by SDS-PAGE electrophoresis to be 38 kDa. It could become a potential candidate in the treatment of atherosclerosis. |
| first_indexed | 2024-12-16T14:54:40Z |
| format | Article |
| id | doaj.art-3b6a98e8834143ddb05d8128b5270e2c |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-16T14:54:40Z |
| publishDate | 2013-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-3b6a98e8834143ddb05d8128b5270e2c2022-12-21T22:27:29ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392013-01-0165276777210.2298/ABS1302767ZA novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn.Zhou XiaoliHuang LinTang WenZhou YimingWang QingLi ZongjieBuckwheat seeds contain many kinds of functional compounds that are of benefit to patients with cardiovascular disease. In this research, a water-soluble buckwheat protein was isolated and purified through a DEAE-Sepharose anion exchange column and Sephadex G-75 gel chromatography. The isolated buckwheat protein fractions exhibited hypocholesterolemic activity in a HepG2 cell model and demonstrated prominent bile acid salt-binding activity in an in vitro assay. The antioxidative activity of protein fractions with hypolipidemic effects was detected in a free radical scavenging experiment. The buckwheat protein fraction with the most obvious hypolipidemic activity and free radical scavenging activity was named as WSBWP. Its molecular weight was estimated by SDS-PAGE electrophoresis to be 38 kDa. It could become a potential candidate in the treatment of atherosclerosis.http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302767Z.pdfBuckwheat proteinatherosclerosishypocholesterolemic activitybile acid salt binding activityfree radical scavenging activity |
| spellingShingle | Zhou Xiaoli Huang Lin Tang Wen Zhou Yiming Wang Qing Li Zongjie A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. Archives of Biological Sciences Buckwheat protein atherosclerosis hypocholesterolemic activity bile acid salt binding activity free radical scavenging activity |
| title | A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. |
| title_full | A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. |
| title_fullStr | A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. |
| title_full_unstemmed | A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. |
| title_short | A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. |
| title_sort | novel buckwheat protein with a beneficial effect in atherosclerosis was purified from fagopyrum tataricum l gaertn |
| topic | Buckwheat protein atherosclerosis hypocholesterolemic activity bile acid salt binding activity free radical scavenging activity |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302767Z.pdf |
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