Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2015-02-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100001&lng=en&tlng=en |
| _version_ | 1818765757921624064 |
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| author | Francine Silva Antelo Jorge Alberto Vieira Costa Susana Juliano Kalil |
| author_facet | Francine Silva Antelo Jorge Alberto Vieira Costa Susana Juliano Kalil |
| author_sort | Francine Silva Antelo |
| collection | DOAJ |
| description | C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG 1500 or 4% (w/w) PEG 8000 (g/gmol) and 23 or 22.5% (w/w) of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the purification factor for the system with PEG 1500. The ATPS with 4% (w/w) PEG 4000 or 4% (w/w) PEG 6000 and 21% (w/w) phosphate resulted purification factors of 2.1 and 2.2-fold, recovering 100% and 73.5%, respectively of C-phycocyanin in the top phase. |
| first_indexed | 2024-12-18T08:23:10Z |
| format | Article |
| id | doaj.art-3b6afeb6954e454ebe53ce3cfbaf84b4 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-18T08:23:10Z |
| publishDate | 2015-02-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-3b6afeb6954e454ebe53ce3cfbaf84b42022-12-21T21:14:42ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-02-0158111110.1590/S1516-8913201502621S1516-89132015000100001Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental designFrancine Silva AnteloJorge Alberto Vieira CostaSusana Juliano KalilC-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG 1500 or 4% (w/w) PEG 8000 (g/gmol) and 23 or 22.5% (w/w) of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the purification factor for the system with PEG 1500. The ATPS with 4% (w/w) PEG 4000 or 4% (w/w) PEG 6000 and 21% (w/w) phosphate resulted purification factors of 2.1 and 2.2-fold, recovering 100% and 73.5%, respectively of C-phycocyanin in the top phase.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100001&lng=en&tlng=enC-phycocyaninPurification FactorProteinPolyethylene glycol |
| spellingShingle | Francine Silva Antelo Jorge Alberto Vieira Costa Susana Juliano Kalil Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design Brazilian Archives of Biology and Technology C-phycocyanin Purification Factor Protein Polyethylene glycol |
| title | Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design |
| title_full | Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design |
| title_fullStr | Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design |
| title_full_unstemmed | Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design |
| title_short | Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design |
| title_sort | purification of c phycocyanin from spirulina platensis in aqueous two phase systems using an experimental design |
| topic | C-phycocyanin Purification Factor Protein Polyethylene glycol |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100001&lng=en&tlng=en |
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