Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves
Summary: Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acidâ a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | JACC: Basic to Translational Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452302X16301309 |
| _version_ | 1818940036046913536 |
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| author | Ryan S. Hennessy, MD Soumen Jana, PhD Brandon J. Tefft, PhD Meghana R. Helder, MD Melissa D. Young, PhD Rebecca R. Hennessy, MD Nicholas J. Stoyles, BS Amir Lerman, MD |
| author_facet | Ryan S. Hennessy, MD Soumen Jana, PhD Brandon J. Tefft, PhD Meghana R. Helder, MD Melissa D. Young, PhD Rebecca R. Hennessy, MD Nicholas J. Stoyles, BS Amir Lerman, MD |
| author_sort | Ryan S. Hennessy, MD |
| collection | DOAJ |
| description | Summary: Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acidâ and supercritical carbon dioxideâtreated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxideâtreated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues. Key Words: decellularized, decontamination, heart valve, tensile properties, tissue engineering |
| first_indexed | 2024-12-20T06:33:15Z |
| format | Article |
| id | doaj.art-3b6b103bf08744bf852e10b9c4058d45 |
| institution | Directory Open Access Journal |
| issn | 2452-302X |
| language | English |
| last_indexed | 2024-12-20T06:33:15Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JACC: Basic to Translational Science |
| spelling | doaj.art-3b6b103bf08744bf852e10b9c4058d452022-12-21T19:50:04ZengElsevierJACC: Basic to Translational Science2452-302X2017-02-01217184Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart ValvesRyan S. Hennessy, MD0Soumen Jana, PhD1Brandon J. Tefft, PhD2Meghana R. Helder, MD3Melissa D. Young, PhD4Rebecca R. Hennessy, MD5Nicholas J. Stoyles, BS6Amir Lerman, MD7Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Diseases, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Diseases, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Surgery, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Diseases, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Diseases, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Diseases, Mayo Clinic, Rochester, MinnesotaDivision of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota; Address for correspondence: Dr. Amir Lerman, Division of Cardiovascular Diseases, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905.Summary: Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acidâ and supercritical carbon dioxideâtreated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxideâtreated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues. Key Words: decellularized, decontamination, heart valve, tensile properties, tissue engineeringhttp://www.sciencedirect.com/science/article/pii/S2452302X16301309 |
| spellingShingle | Ryan S. Hennessy, MD Soumen Jana, PhD Brandon J. Tefft, PhD Meghana R. Helder, MD Melissa D. Young, PhD Rebecca R. Hennessy, MD Nicholas J. Stoyles, BS Amir Lerman, MD Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves JACC: Basic to Translational Science |
| title | Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves |
| title_full | Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves |
| title_fullStr | Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves |
| title_full_unstemmed | Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves |
| title_short | Supercritical Carbon DioxideâBased Sterilization of Decellularized Heart Valves |
| title_sort | supercritical carbon dioxideabased sterilization of decellularized heart valves |
| url | http://www.sciencedirect.com/science/article/pii/S2452302X16301309 |
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