AtGCS promoter-driven clustered regularly interspaced short palindromic repeats/Cas9 highly efficiently generates homozygous/biallelic mutations in the transformed roots by Agrobacterium rhizogenes–mediated transformation

AtGCS promoter-driven clustered regularly interspaced short palindromic repeats/Cas9 highly efficiently generates homozygous/biallelic mutations in the transformed roots by Agrobacterium rhizogenes–mediated transformation

Agrobacterium rhizogenes–mediated (ARM) transformation is an efficient and powerful tool to generate transgenic roots to study root-related biology. For loss-of-function studies, transgenic-root-induced indel mutations by CRISPR/Cas9 only with homozygous/biallelic mutagenesis can exhibit mutant phen...

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Bibliographic Details
Main Authors: Shuang Liu, Xiuyuan Wang, Qianqian Li, Wentao Peng, Zunmian Zhang, Pengfei Chu, Shangjing Guo, Yinglun Fan, Shanhua Lyu
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
agrobacterium rhizogenes–mediated transformation (ARM)
genome editing
homozygous/biallelic mutation
hairy root
gamma-glutamylcysteine synthetase gene
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.952428/full

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