Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/11/11/745 |
| _version_ | 1797509356205375488 |
|---|---|
| author | Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam Benjamin Marie |
| author_facet | Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam Benjamin Marie |
| author_sort | Damien Le Moigne |
| collection | DOAJ |
| description | Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying the metabolite profile is one way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics of the whole metabolome depended on the growth phase and various abiotic factors such as light intensity and temperature. The cyanobacterium, <i>Aliinostoc</i> sp. PMC 882.14, was selected for its large number of biosynthetic gene clusters. One group of cells was grown under normal conditions as a control, while other groups were grown under higher light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted analysis to investigate metabolite dynamics, and significant variation was found between exponential and stationary phases, regardless of culture conditions. In the higher light group, the synthesis of several metabolites, including shinorine, was induced while other metabolites, such as microviridins, were synthesized under higher temperature conditions. Among highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of considering the physiological state of cyanobacteria for comparative global metabolomics and studies of the regulatory processes involved in production of specific metabolites. Our results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites. |
| first_indexed | 2024-03-10T05:16:33Z |
| format | Article |
| id | doaj.art-3b6f52a5d9fa4365b5a1d2fe8f418ac9 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T05:16:33Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-3b6f52a5d9fa4365b5a1d2fe8f418ac92023-11-23T00:20:54ZengMDPI AGMetabolites2218-19892021-10-01111174510.3390/metabo11110745Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature VariationsDamien Le Moigne0Justine Demay1Anita Reinhardt2Cécile Bernard3Sandra Kim Tiam4Benjamin Marie5UMR7245 Molécules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Muséum National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, FranceUMR7245 Molécules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Muséum National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, FranceThermes de Balaruc-Les-Bains, 1 Rue du Mont Saint-Clair BP 45, 34540 Balaruc-Les-Bains, FranceUMR7245 Molécules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Muséum National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, FranceUMR7245 Molécules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Muséum National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, FranceUMR7245 Molécules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Muséum National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, FranceCyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying the metabolite profile is one way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics of the whole metabolome depended on the growth phase and various abiotic factors such as light intensity and temperature. The cyanobacterium, <i>Aliinostoc</i> sp. PMC 882.14, was selected for its large number of biosynthetic gene clusters. One group of cells was grown under normal conditions as a control, while other groups were grown under higher light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted analysis to investigate metabolite dynamics, and significant variation was found between exponential and stationary phases, regardless of culture conditions. In the higher light group, the synthesis of several metabolites, including shinorine, was induced while other metabolites, such as microviridins, were synthesized under higher temperature conditions. Among highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of considering the physiological state of cyanobacteria for comparative global metabolomics and studies of the regulatory processes involved in production of specific metabolites. Our results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites.https://www.mdpi.com/2218-1989/11/11/745cyanobacteriametabolomicshigh-resolution mass spectrometrysecondary metabolite inductionculture conditions |
| spellingShingle | Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam Benjamin Marie Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations Metabolites cyanobacteria metabolomics high-resolution mass spectrometry secondary metabolite induction culture conditions |
| title | Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations |
| title_full | Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations |
| title_fullStr | Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations |
| title_full_unstemmed | Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations |
| title_short | Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations |
| title_sort | dynamics of the metabolome of i aliinostoc i sp pmc 882 14 in response to light and temperature variations |
| topic | cyanobacteria metabolomics high-resolution mass spectrometry secondary metabolite induction culture conditions |
| url | https://www.mdpi.com/2218-1989/11/11/745 |
| work_keys_str_mv | AT damienlemoigne dynamicsofthemetabolomeofialiinostocisppmc88214inresponsetolightandtemperaturevariations AT justinedemay dynamicsofthemetabolomeofialiinostocisppmc88214inresponsetolightandtemperaturevariations AT anitareinhardt dynamicsofthemetabolomeofialiinostocisppmc88214inresponsetolightandtemperaturevariations AT cecilebernard dynamicsofthemetabolomeofialiinostocisppmc88214inresponsetolightandtemperaturevariations AT sandrakimtiam dynamicsofthemetabolomeofialiinostocisppmc88214inresponsetolightandtemperaturevariations AT benjaminmarie dynamicsofthemetabolomeofialiinostocisppmc88214inresponsetolightandtemperaturevariations |