Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes
Although the skin is constantly subjected to endogenous and exogenous stress, it maintains a homeostatic state through wound repair and regeneration pathways. Treatment for skin diseases and injury requires a significant understanding of the various mechanisms and interactions that occur within skin...
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MDPI AG
2021-12-01
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| author | Pradeep Bhartiya Kai Masur Debarati Shome Neha Kaushik Linh N. Nguyen Nagendra Kumar Kaushik Eun Ha Choi |
| author_facet | Pradeep Bhartiya Kai Masur Debarati Shome Neha Kaushik Linh N. Nguyen Nagendra Kumar Kaushik Eun Ha Choi |
| author_sort | Pradeep Bhartiya |
| collection | DOAJ |
| description | Although the skin is constantly subjected to endogenous and exogenous stress, it maintains a homeostatic state through wound repair and regeneration pathways. Treatment for skin diseases and injury requires a significant understanding of the various mechanisms and interactions that occur within skin cells. Keratinocytes and fibroblasts interact with each other and act as key players in the repair process. Although fibroblasts and keratinocytes are widely studied in wound healing and skin remodeling under different conditions, the influence of redox stress on keratinocyte-fibroblast crosstalk has not been thoroughly investigated. In this study, we used cold atmospheric plasma (CAP) to generate and deliver oxidative stress to keratinocytes and fibroblasts and to assess its impact on their interactions. To this end, we used a well-established in vitro 3D co-culture model imitating a realistic scenario. Our study shows that low CAP exposure is biocompatible and does not affect the viability or energetics of fibroblasts and keratinocytes. Exposure to low doses of CAP enhanced the proliferation rate of cells and stimulated the expression of key genes (<i>KGF</i>, <i>MMP2</i>, <i>GMCSF</i>, <i>IL-6</i>, and <i>IL-8</i>) in fibroblasts, indicating the activation and initiation of the skin repair process. Additionally, enhanced migration was observed under co-culture conditions under the given redox stress conditions, and expression of the upstream regulator and the effectors of the Hippo pathway (YAP and CYR61, respectively), which are associated with enhanced migration, were elevated. Overall, this study reinforces the application of CAP and redox stress in skin repair physiology. |
| first_indexed | 2024-03-10T04:33:33Z |
| format | Article |
| id | doaj.art-3b6fa2ff39364c378320bfb8c52bd204 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T04:33:33Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-3b6fa2ff39364c378320bfb8c52bd2042023-11-23T03:54:33ZengMDPI AGBiology2079-77372021-12-011012133810.3390/biology10121338Influence of Redox Stress on Crosstalk between Fibroblasts and KeratinocytesPradeep Bhartiya0Kai Masur1Debarati Shome2Neha Kaushik3Linh N. Nguyen4Nagendra Kumar Kaushik5Eun Ha Choi6Plasma Bioscience Research Center, Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaZIK <i>Plasmatis</i>, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyZIK <i>Plasmatis</i>, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyDepartment of Biotechnology, College of Engineering, University of Suwon, Hwaseong 18323, KoreaPlasma Bioscience Research Center, Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaPlasma Bioscience Research Center, Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaPlasma Bioscience Research Center, Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, KoreaAlthough the skin is constantly subjected to endogenous and exogenous stress, it maintains a homeostatic state through wound repair and regeneration pathways. Treatment for skin diseases and injury requires a significant understanding of the various mechanisms and interactions that occur within skin cells. Keratinocytes and fibroblasts interact with each other and act as key players in the repair process. Although fibroblasts and keratinocytes are widely studied in wound healing and skin remodeling under different conditions, the influence of redox stress on keratinocyte-fibroblast crosstalk has not been thoroughly investigated. In this study, we used cold atmospheric plasma (CAP) to generate and deliver oxidative stress to keratinocytes and fibroblasts and to assess its impact on their interactions. To this end, we used a well-established in vitro 3D co-culture model imitating a realistic scenario. Our study shows that low CAP exposure is biocompatible and does not affect the viability or energetics of fibroblasts and keratinocytes. Exposure to low doses of CAP enhanced the proliferation rate of cells and stimulated the expression of key genes (<i>KGF</i>, <i>MMP2</i>, <i>GMCSF</i>, <i>IL-6</i>, and <i>IL-8</i>) in fibroblasts, indicating the activation and initiation of the skin repair process. Additionally, enhanced migration was observed under co-culture conditions under the given redox stress conditions, and expression of the upstream regulator and the effectors of the Hippo pathway (YAP and CYR61, respectively), which are associated with enhanced migration, were elevated. Overall, this study reinforces the application of CAP and redox stress in skin repair physiology.https://www.mdpi.com/2079-7737/10/12/1338skin homeostasisphysical plasmacell migration |
| spellingShingle | Pradeep Bhartiya Kai Masur Debarati Shome Neha Kaushik Linh N. Nguyen Nagendra Kumar Kaushik Eun Ha Choi Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes Biology skin homeostasis physical plasma cell migration |
| title | Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes |
| title_full | Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes |
| title_fullStr | Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes |
| title_full_unstemmed | Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes |
| title_short | Influence of Redox Stress on Crosstalk between Fibroblasts and Keratinocytes |
| title_sort | influence of redox stress on crosstalk between fibroblasts and keratinocytes |
| topic | skin homeostasis physical plasma cell migration |
| url | https://www.mdpi.com/2079-7737/10/12/1338 |
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