Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation
Introduction: Pericardial fluid is enriched with biologically active molecules of cardiovascular origin including microRNAs. Investigation of the disease-specific extracellular microRNAs could shed light on the molecular processes underlying disease development. Arrhythmogenic right ventricular card...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2021.647812/full |
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| author | Aleksandr A. Khudiakov Daniil D. Panshin Yulia V. Fomicheva Anastasia A. Knyazeva Ksenia A. Simonova Dmitry S. Lebedev Dmitry S. Lebedev Evgeny N. Mikhaylov Evgeny N. Mikhaylov Anna A. Kostareva Anna A. Kostareva |
| author_facet | Aleksandr A. Khudiakov Daniil D. Panshin Yulia V. Fomicheva Anastasia A. Knyazeva Ksenia A. Simonova Dmitry S. Lebedev Dmitry S. Lebedev Evgeny N. Mikhaylov Evgeny N. Mikhaylov Anna A. Kostareva Anna A. Kostareva |
| author_sort | Aleksandr A. Khudiakov |
| collection | DOAJ |
| description | Introduction: Pericardial fluid is enriched with biologically active molecules of cardiovascular origin including microRNAs. Investigation of the disease-specific extracellular microRNAs could shed light on the molecular processes underlying disease development. Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited heart disease characterized by life-threatening arrhythmias and progressive heart failure development. The current data about the association between microRNAs and ARVC development are limited.Methods and Results: We performed small RNA sequence analysis of microRNAs of pericardial fluid samples obtained during transcutaneous epicardial access for ventricular tachycardia (VT) ablation of six patients with definite ARVC and three post-infarction VT patients. Disease-associated microRNAs of pericardial fluid were identified. Five microRNAs (hsa-miR-1-3p, hsa-miR-21-5p, hsa-miR-122-5p, hsa-miR-206, and hsa-miR-3679-5p) were found to be differentially expressed between patients with ARVC and patients with post-infarction VT. Enrichment analysis of differentially expressed microRNAs revealed their close linkage to cardiac diseases.Conclusion: Our data extend the knowledge of pericardial fluid microRNA composition and highlight five pericardial fluid microRNAs potentially linked to ARVC pathogenesis. Further studies are required to confirm the use of pericardial fluid RNA sequencing in differential diagnosis of ARVC. |
| first_indexed | 2024-12-17T08:40:48Z |
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| id | doaj.art-3b72b83690574ef18b7a1add06cc8d8c |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-12-17T08:40:48Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-3b72b83690574ef18b7a1add06cc8d8c2022-12-21T21:56:21ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2021-03-01810.3389/fcvm.2021.647812647812Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia AblationAleksandr A. Khudiakov0Daniil D. Panshin1Yulia V. Fomicheva2Anastasia A. Knyazeva3Ksenia A. Simonova4Dmitry S. Lebedev5Dmitry S. Lebedev6Evgeny N. Mikhaylov7Evgeny N. Mikhaylov8Anna A. Kostareva9Anna A. Kostareva10Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaDepartment of Bioengineering Systems, Saint Petersburg Electrotechnical University “LETI”, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaDepartment of Bioengineering Systems, Saint Petersburg Electrotechnical University “LETI”, Saint Petersburg, RussiaInstitute of Molecular Biology and Genetics, Almazov National Medical Research Centre, Saint Petersburg, RussiaDepartment of Women's and Children's Health, Center for Molecular Medicine, Karolinska Institute, Stockholm, SwedenIntroduction: Pericardial fluid is enriched with biologically active molecules of cardiovascular origin including microRNAs. Investigation of the disease-specific extracellular microRNAs could shed light on the molecular processes underlying disease development. Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited heart disease characterized by life-threatening arrhythmias and progressive heart failure development. The current data about the association between microRNAs and ARVC development are limited.Methods and Results: We performed small RNA sequence analysis of microRNAs of pericardial fluid samples obtained during transcutaneous epicardial access for ventricular tachycardia (VT) ablation of six patients with definite ARVC and three post-infarction VT patients. Disease-associated microRNAs of pericardial fluid were identified. Five microRNAs (hsa-miR-1-3p, hsa-miR-21-5p, hsa-miR-122-5p, hsa-miR-206, and hsa-miR-3679-5p) were found to be differentially expressed between patients with ARVC and patients with post-infarction VT. Enrichment analysis of differentially expressed microRNAs revealed their close linkage to cardiac diseases.Conclusion: Our data extend the knowledge of pericardial fluid microRNA composition and highlight five pericardial fluid microRNAs potentially linked to ARVC pathogenesis. Further studies are required to confirm the use of pericardial fluid RNA sequencing in differential diagnosis of ARVC.https://www.frontiersin.org/articles/10.3389/fcvm.2021.647812/fullmicroRNAsmall RNA sequencingpericardial fluidarrhythmogenic (right ventricular) cardiomyopathyexpression analyses |
| spellingShingle | Aleksandr A. Khudiakov Daniil D. Panshin Yulia V. Fomicheva Anastasia A. Knyazeva Ksenia A. Simonova Dmitry S. Lebedev Dmitry S. Lebedev Evgeny N. Mikhaylov Evgeny N. Mikhaylov Anna A. Kostareva Anna A. Kostareva Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation Frontiers in Cardiovascular Medicine microRNA small RNA sequencing pericardial fluid arrhythmogenic (right ventricular) cardiomyopathy expression analyses |
| title | Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation |
| title_full | Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation |
| title_fullStr | Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation |
| title_full_unstemmed | Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation |
| title_short | Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation |
| title_sort | different expressions of pericardial fluid micrornas in patients with arrhythmogenic right ventricular cardiomyopathy and ischemic heart disease undergoing ventricular tachycardia ablation |
| topic | microRNA small RNA sequencing pericardial fluid arrhythmogenic (right ventricular) cardiomyopathy expression analyses |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2021.647812/full |
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