Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer
Despite a global decrease in colorectal cancer (CRC) incidence, the prevalence of early-onset colorectal cancer (EOCRC), or those occurring in individuals before the age of 50, has steadily increased over the past several decades. When compared to later onset colorectal cancer (LOCRC) in individuals...
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MDPI AG
2022-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/9/1223 |
| _version_ | 1797490727778779136 |
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| author | Olivia M. Marx Marc M. Mankarious Melanie A. Eshelman Wei Ding Walter A. Koltun Gregory S. Yochum |
| author_facet | Olivia M. Marx Marc M. Mankarious Melanie A. Eshelman Wei Ding Walter A. Koltun Gregory S. Yochum |
| author_sort | Olivia M. Marx |
| collection | DOAJ |
| description | Despite a global decrease in colorectal cancer (CRC) incidence, the prevalence of early-onset colorectal cancer (EOCRC), or those occurring in individuals before the age of 50, has steadily increased over the past several decades. When compared to later onset colorectal cancer (LOCRC) in individuals over 50, our understanding of the genetic and molecular underpinnings of EOCRCs is limited. Here, we conducted transcriptomic analyses of patient-matched normal colonic segments and tumors to identify gene expression programs involved in carcinogenesis. Amongst differentially expressed genes, we found increased expression of the <i>c-MYC</i> proto-oncogene (<i>MYC</i>) and its downstream targets in tumor samples. We identified tumors with high and low differential <i>MYC</i> expression and found patients with high-<i>MYC</i> tumors were older and overweight or obese. We also detected elevated expression of the <i>PVT1</i> long-non-coding RNA (lncRNA) in most tumors and found gains in copy number for both <i>MYC</i> and <i>PVT1</i> gene loci in 35% of tumors evaluated. Our transcriptome analyses indicate that EOCRC can be sub-classified into groups based on differential <i>MYC</i> expression and suggest that deregulated <i>MYC</i> contributes to CRCs that develop in younger patients. |
| first_indexed | 2024-03-10T00:37:09Z |
| format | Article |
| id | doaj.art-3b72e533a9a5400990d33be42b34f3f7 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T00:37:09Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-3b72e533a9a5400990d33be42b34f3f72023-11-23T15:14:56ZengMDPI AGBiomolecules2218-273X2022-09-01129122310.3390/biom12091223Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal CancerOlivia M. Marx0Marc M. Mankarious1Melanie A. Eshelman2Wei Ding3Walter A. Koltun4Gregory S. Yochum5Department of Biochemistry & Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USADepartment of Surgery, Division of Colon & Rectal Surgery, Pennsylvania State University College of Medicine, Hershey, PA 17033, USADepartment of Pediatrics, Division of Hematology & Oncology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USADepartment of Surgery, Division of Colon & Rectal Surgery, Pennsylvania State University College of Medicine, Hershey, PA 17033, USADepartment of Surgery, Division of Colon & Rectal Surgery, Pennsylvania State University College of Medicine, Hershey, PA 17033, USADepartment of Biochemistry & Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USADespite a global decrease in colorectal cancer (CRC) incidence, the prevalence of early-onset colorectal cancer (EOCRC), or those occurring in individuals before the age of 50, has steadily increased over the past several decades. When compared to later onset colorectal cancer (LOCRC) in individuals over 50, our understanding of the genetic and molecular underpinnings of EOCRCs is limited. Here, we conducted transcriptomic analyses of patient-matched normal colonic segments and tumors to identify gene expression programs involved in carcinogenesis. Amongst differentially expressed genes, we found increased expression of the <i>c-MYC</i> proto-oncogene (<i>MYC</i>) and its downstream targets in tumor samples. We identified tumors with high and low differential <i>MYC</i> expression and found patients with high-<i>MYC</i> tumors were older and overweight or obese. We also detected elevated expression of the <i>PVT1</i> long-non-coding RNA (lncRNA) in most tumors and found gains in copy number for both <i>MYC</i> and <i>PVT1</i> gene loci in 35% of tumors evaluated. Our transcriptome analyses indicate that EOCRC can be sub-classified into groups based on differential <i>MYC</i> expression and suggest that deregulated <i>MYC</i> contributes to CRCs that develop in younger patients.https://www.mdpi.com/2218-273X/12/9/1223early-onset colorectal cancerRNA-sequencingtranscriptomeMYCPVT1 |
| spellingShingle | Olivia M. Marx Marc M. Mankarious Melanie A. Eshelman Wei Ding Walter A. Koltun Gregory S. Yochum Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer Biomolecules early-onset colorectal cancer RNA-sequencing transcriptome MYC PVT1 |
| title | Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer |
| title_full | Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer |
| title_fullStr | Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer |
| title_full_unstemmed | Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer |
| title_short | Transcriptome Analyses Identify Deregulated <i>MYC</i> in Early Onset Colorectal Cancer |
| title_sort | transcriptome analyses identify deregulated i myc i in early onset colorectal cancer |
| topic | early-onset colorectal cancer RNA-sequencing transcriptome MYC PVT1 |
| url | https://www.mdpi.com/2218-273X/12/9/1223 |
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