PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA
Objective of the study is to prepare rabbit monospecific anti-abortus serum to a Brucella S-form thermoextract, as well as to evaluate its activity and specificity. Materials and methods. Utilized was polyvalent hyperimmune serum obtained to Brucella thermoextract. It was adsorbed by a strain of het...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2017-06-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/397 |
| _version_ | 1797220857781682176 |
|---|---|
| author | L. M. Мikhailov N. L. Barannikova L. E. Tokareva N. M. Andreevskaya V. A. Мikhailova V. I. Kuznetsov K. Yu. Yastremskaya S. V. Balakhonov |
| author_facet | L. M. Мikhailov N. L. Barannikova L. E. Tokareva N. M. Andreevskaya V. A. Мikhailova V. I. Kuznetsov K. Yu. Yastremskaya S. V. Balakhonov |
| author_sort | L. M. Мikhailov |
| collection | DOAJ |
| description | Objective of the study is to prepare rabbit monospecific anti-abortus serum to a Brucella S-form thermoextract, as well as to evaluate its activity and specificity. Materials and methods. Utilized was polyvalent hyperimmune serum obtained to Brucella thermoextract. It was adsorbed by a strain of heterologous species Brucella melitensis 16 N. Reference, vaccinal and field Brucella strains were used to estimate the activity and specificity of the serum produced. Results and conclusions. This serum possesses high activity (1:160, 1:320 titer) and specificity, and the results completely coincide with the data specified in the table of differential properties presented in МR 3.1.7.1189-03 “Prophylaxis and laboratory diagnostics of human brucellosis». The outlined method for manufacturing monospecific Brucella anti-abortus serum permits for a considerable decrease in biological hazard for personnel involved in the technological operations. |
| first_indexed | 2024-03-08T13:42:10Z |
| format | Article |
| id | doaj.art-3b792d93c8434fff9ef57722577cb46c |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:12Z |
| publishDate | 2017-06-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-3b792d93c8434fff9ef57722577cb46c2024-04-05T16:47:12ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2017-06-0102788010.21055/0370-1069-2017-2-78-80397PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLAL. M. Мikhailov0N. L. Barannikova1L. E. Tokareva2N. M. Andreevskaya3V. A. Мikhailova4V. I. Kuznetsov5K. Yu. Yastremskaya6S. V. Balakhonov7Irkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastIrkutsk Research Anti-Plague Institute of Siberia and Far EastObjective of the study is to prepare rabbit monospecific anti-abortus serum to a Brucella S-form thermoextract, as well as to evaluate its activity and specificity. Materials and methods. Utilized was polyvalent hyperimmune serum obtained to Brucella thermoextract. It was adsorbed by a strain of heterologous species Brucella melitensis 16 N. Reference, vaccinal and field Brucella strains were used to estimate the activity and specificity of the serum produced. Results and conclusions. This serum possesses high activity (1:160, 1:320 titer) and specificity, and the results completely coincide with the data specified in the table of differential properties presented in МR 3.1.7.1189-03 “Prophylaxis and laboratory diagnostics of human brucellosis». The outlined method for manufacturing monospecific Brucella anti-abortus serum permits for a considerable decrease in biological hazard for personnel involved in the technological operations.https://journal.microbe.ru/jour/article/view/397brucellathermoextracts-formmonospecific serum |
| spellingShingle | L. M. Мikhailov N. L. Barannikova L. E. Tokareva N. M. Andreevskaya V. A. Мikhailova V. I. Kuznetsov K. Yu. Yastremskaya S. V. Balakhonov PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA Проблемы особо опасных инфекций brucella thermoextract s-form monospecific serum |
| title | PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA |
| title_full | PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA |
| title_fullStr | PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA |
| title_full_unstemmed | PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA |
| title_short | PREPARATION OF MONOSPECIFIC BRUCELLA ANTI-ABORTUS SERUM BASED ON THERMOEXTRACT FROM S-FORM BRUCELLA |
| title_sort | preparation of monospecific brucella anti abortus serum based on thermoextract from s form brucella |
| topic | brucella thermoextract s-form monospecific serum |
| url | https://journal.microbe.ru/jour/article/view/397 |
| work_keys_str_mv | AT lmmikhailov preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT nlbarannikova preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT letokareva preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT nmandreevskaya preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT vamikhailova preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT vikuznetsov preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT kyuyastremskaya preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella AT svbalakhonov preparationofmonospecificbrucellaantiabortusserumbasedonthermoextractfromsformbrucella |