The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization
The expression of the <i>col11a1a</i> gene is essential for normal skeletal development, affecting both cartilage and bone. Loss of function mutations have been shown to cause abnormalities in the growth plate of long bones, as well as in craniofacial development. However, the specific e...
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MDPI AG
2022-09-01
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| Series: | Journal of Developmental Biology |
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| Online Access: | https://www.mdpi.com/2221-3759/10/4/40 |
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| author | Jonathon C. Reeck Julia Thom Oxford |
| author_facet | Jonathon C. Reeck Julia Thom Oxford |
| author_sort | Jonathon C. Reeck |
| collection | DOAJ |
| description | The expression of the <i>col11a1a</i> gene is essential for normal skeletal development, affecting both cartilage and bone. Loss of function mutations have been shown to cause abnormalities in the growth plate of long bones, as well as in craniofacial development. However, the specific effects on Meckel’s cartilage have not been well studied. To further understand the effect of <i>col11a1a</i> gene function, we analyzed the developing jaw in zebrafish using gene knockdown by the injection of an antisense morpholino oligonucleotide using transgenic Tg(sp7:EGFP) and Tg(Fli1a:EGFP) EGFP reporter fish, as well as wildtype AB zebrafish. Our results demonstrate that zebrafish <i>col11a1a</i> knockdown impairs the cellular organization of Meckel’s cartilage in the developing jaw and alters the bone formation that occurs adjacent to the Meckel’s cartilage. These results suggest roles for Col11a1a protein in cartilage intermediates of bone development, the subsequent mineralization of the bony collar of long bones, and that which occurs adjacent to Meckel’s cartilage in the developing jaw. |
| first_indexed | 2024-03-09T16:15:39Z |
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| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-03-09T16:15:39Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-3b79519db7484d39a994d67797e7711f2023-11-24T15:48:22ZengMDPI AGJournal of Developmental Biology2221-37592022-09-011044010.3390/jdb10040040The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and MineralizationJonathon C. Reeck0Julia Thom Oxford1Department of Biological Sciences, Biomolecular Research Center, Center of Biomedical Research Excellence in Matrix Biology, Boise State University, Boise, ID 83725, USADepartment of Biological Sciences, Biomolecular Research Center, Center of Biomedical Research Excellence in Matrix Biology, Boise State University, Boise, ID 83725, USAThe expression of the <i>col11a1a</i> gene is essential for normal skeletal development, affecting both cartilage and bone. Loss of function mutations have been shown to cause abnormalities in the growth plate of long bones, as well as in craniofacial development. However, the specific effects on Meckel’s cartilage have not been well studied. To further understand the effect of <i>col11a1a</i> gene function, we analyzed the developing jaw in zebrafish using gene knockdown by the injection of an antisense morpholino oligonucleotide using transgenic Tg(sp7:EGFP) and Tg(Fli1a:EGFP) EGFP reporter fish, as well as wildtype AB zebrafish. Our results demonstrate that zebrafish <i>col11a1a</i> knockdown impairs the cellular organization of Meckel’s cartilage in the developing jaw and alters the bone formation that occurs adjacent to the Meckel’s cartilage. These results suggest roles for Col11a1a protein in cartilage intermediates of bone development, the subsequent mineralization of the bony collar of long bones, and that which occurs adjacent to Meckel’s cartilage in the developing jaw.https://www.mdpi.com/2221-3759/10/4/40Meckel’s cartilagejaw developmentmineralizationcraniofacialzebrafishStickler syndrome type II |
| spellingShingle | Jonathon C. Reeck Julia Thom Oxford The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization Journal of Developmental Biology Meckel’s cartilage jaw development mineralization craniofacial zebrafish Stickler syndrome type II |
| title | The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization |
| title_full | The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization |
| title_fullStr | The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization |
| title_full_unstemmed | The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization |
| title_short | The Shape of the Jaw—Zebrafish Col11a1a Regulates Meckel’s Cartilage Morphogenesis and Mineralization |
| title_sort | shape of the jaw zebrafish col11a1a regulates meckel s cartilage morphogenesis and mineralization |
| topic | Meckel’s cartilage jaw development mineralization craniofacial zebrafish Stickler syndrome type II |
| url | https://www.mdpi.com/2221-3759/10/4/40 |
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