Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape
We surveyed infection by <i>Trypanosoma</i> spp. and <i>Leishmania</i> spp. in small wild mammals from Cumari, Goiás State aiming to investigate the diversity of trypanosomatid in a modified landscape of the Brazilian Cerrado (and possible infection overlapping with...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/4/190 |
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| author | Elida M. V. Brandão Samanta C. C. Xavier Jeiel G. Carvalhaes Paulo S. D'Andrea Frederico G. Lemos Fernanda C. Azevedo Renata Cássia-Pires Ana M. Jansen André L. R. Roque |
| author_facet | Elida M. V. Brandão Samanta C. C. Xavier Jeiel G. Carvalhaes Paulo S. D'Andrea Frederico G. Lemos Fernanda C. Azevedo Renata Cássia-Pires Ana M. Jansen André L. R. Roque |
| author_sort | Elida M. V. Brandão |
| collection | DOAJ |
| description | We surveyed infection by <i>Trypanosoma</i> spp. and <i>Leishmania</i> spp. in small wild mammals from Cumari, Goiás State aiming to investigate the diversity of trypanosomatid in a modified landscape of the Brazilian Cerrado (and possible infection overlapping with canids from the same area). Blood, skin, spleen, and liver samples were collected for parasitological, serological, and molecular assays. <i>Gracilinanus agilis</i> was the most abundant species (<i>N</i> = 70; 48.6%) and it was the only one with patent parasitemia. Characterization by mini-exon and 18SrDNA targets were achieved in 7/10 hemocultures with positive fresh blood examination, which confirmed the <i>T. cruzi</i> infection by Discrete Typing Units (DTU) TcI in single (<i>N</i> = 2) and mixed infections with other DTUs (<i>N</i> = 5). <i>T. rangeli</i> and <i>T. dionisii</i> were detected in skin fragments from <i>Didelphis albiventris</i> and <i>Oecomys cleberi,</i> respectively. <i>G. agilis</i> were found to be infected by <i>L. braziliensis</i> and <i>L. guyanensis,</i> while <i>Leishmania</i> sp. DNA was detected in the liver of <i>Oligoryzomys nigripes</i> and <i>Calomys expulsus</i>. Subpatent infection by <i>T. cruzi</i> and <i>Leishmania</i> sp. was serologically detected in 15% and 9% of the small mammal fauna, respectively. Small mammals from Cumari are included in <i>T. cruzi</i> and <i>Leshmania</i> spp. transmission cycles, showing a higher diversity of trypanosomatid species and/or genotypes than that observed in canids of the same agroecosystem. |
| first_indexed | 2024-04-11T21:50:19Z |
| format | Article |
| id | doaj.art-3b7b079918e04d17a22b858650ff68dd |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T21:50:19Z |
| publishDate | 2019-10-01 |
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| series | Pathogens |
| spelling | doaj.art-3b7b079918e04d17a22b858650ff68dd2022-12-22T04:01:16ZengMDPI AGPathogens2076-08172019-10-018419010.3390/pathogens8040190pathogens8040190Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic LandscapeElida M. V. Brandão0Samanta C. C. Xavier1Jeiel G. Carvalhaes2Paulo S. D'Andrea3Frederico G. Lemos4Fernanda C. Azevedo5Renata Cássia-Pires6Ana M. Jansen7André L. R. Roque8Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21040900, BrasilLaboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21040900, BrasilLaboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21040900, BrasilLaboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21040900, BrasilPrograma de Conservação Mamíferos do Cerrado (PCMC)–Unidade Acadêmica Especial de Biotecnologia, Universidade Federal de Goiás/Regional Catalão, Catalão, GO 75704020, BrasilPrograma de Conservação Mamíferos do Cerrado (PCMC)–Unidade Acadêmica Especial de Biotecnologia, Universidade Federal de Goiás/Regional Catalão, Catalão, GO 75704020, BrasilLaboratório de Biologia de Parasitos, Centro de Ciências da Saúde, Departamento de Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte, Natal, RN 59012570, BrasilLaboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21040900, BrasilLaboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ 21040900, BrasilWe surveyed infection by <i>Trypanosoma</i> spp. and <i>Leishmania</i> spp. in small wild mammals from Cumari, Goiás State aiming to investigate the diversity of trypanosomatid in a modified landscape of the Brazilian Cerrado (and possible infection overlapping with canids from the same area). Blood, skin, spleen, and liver samples were collected for parasitological, serological, and molecular assays. <i>Gracilinanus agilis</i> was the most abundant species (<i>N</i> = 70; 48.6%) and it was the only one with patent parasitemia. Characterization by mini-exon and 18SrDNA targets were achieved in 7/10 hemocultures with positive fresh blood examination, which confirmed the <i>T. cruzi</i> infection by Discrete Typing Units (DTU) TcI in single (<i>N</i> = 2) and mixed infections with other DTUs (<i>N</i> = 5). <i>T. rangeli</i> and <i>T. dionisii</i> were detected in skin fragments from <i>Didelphis albiventris</i> and <i>Oecomys cleberi,</i> respectively. <i>G. agilis</i> were found to be infected by <i>L. braziliensis</i> and <i>L. guyanensis,</i> while <i>Leishmania</i> sp. DNA was detected in the liver of <i>Oligoryzomys nigripes</i> and <i>Calomys expulsus</i>. Subpatent infection by <i>T. cruzi</i> and <i>Leishmania</i> sp. was serologically detected in 15% and 9% of the small mammal fauna, respectively. Small mammals from Cumari are included in <i>T. cruzi</i> and <i>Leshmania</i> spp. transmission cycles, showing a higher diversity of trypanosomatid species and/or genotypes than that observed in canids of the same agroecosystem.https://www.mdpi.com/2076-0817/8/4/190agroecosystemsbrazilian cerradomarsupialsrodentscanidsneotropical mammals<i>leishmania</i> sp.<i>trypanosoma cruzi</i> |
| spellingShingle | Elida M. V. Brandão Samanta C. C. Xavier Jeiel G. Carvalhaes Paulo S. D'Andrea Frederico G. Lemos Fernanda C. Azevedo Renata Cássia-Pires Ana M. Jansen André L. R. Roque Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape Pathogens agroecosystems brazilian cerrado marsupials rodents canids neotropical mammals <i>leishmania</i> sp. <i>trypanosoma cruzi</i> |
| title | Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape |
| title_full | Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape |
| title_fullStr | Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape |
| title_full_unstemmed | Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape |
| title_short | Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape |
| title_sort | trypanosomatids in small mammals of an agroecosystem in central brazil another piece in the puzzle of parasite transmission in an anthropogenic landscape |
| topic | agroecosystems brazilian cerrado marsupials rodents canids neotropical mammals <i>leishmania</i> sp. <i>trypanosoma cruzi</i> |
| url | https://www.mdpi.com/2076-0817/8/4/190 |
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