Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/11/782 |
| _version_ | 1797509356430819328 |
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| author | Marcio Antonio Brunetto Bruna Ruberti Doris Pereira Halfen Douglas Segalla Caragelasco Thiago Henrique Annibale Vendramini Vivian Pedrinelli Henrique Tobaro Macedo Juliana Toloi Jeremias Cristiana Fonseca Ferreira Pontieri Fernanda Maria Marins Ocampos Luis Alberto Colnago Marcia Mery Kogika |
| author_facet | Marcio Antonio Brunetto Bruna Ruberti Doris Pereira Halfen Douglas Segalla Caragelasco Thiago Henrique Annibale Vendramini Vivian Pedrinelli Henrique Tobaro Macedo Juliana Toloi Jeremias Cristiana Fonseca Ferreira Pontieri Fernanda Maria Marins Ocampos Luis Alberto Colnago Marcia Mery Kogika |
| author_sort | Marcio Antonio Brunetto |
| collection | DOAJ |
| description | Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluate whether the diet would affect metabolites. Six dogs (5 females; 1 male; 7.47 ± 2.31 years old) with CKD stage 3 or 4 (IRIS) were included. CG consisted of 10 healthy female dogs (5.89 ± 2.57 years old) fed a maintenance diet. Serum metabolites were analyzed by <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectra. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to assess differences in metabolomic profiles between groups and before (CKD-T0) and after renal diet (CKD-T6). Data analysis was performed on SIMCA-P software. Dogs with CKD showed an altered metabolic profile with increased urea, creatinine, creatine, citrate, and lipids. Lactate, branched-chain amino acids (BCAAs), and glutamine were decreased in the CKD group. However, after 6 months of diet, the metabolite profiles of CKD-T0 and CKD-T6 were similar. Metabolomics profile may be useful to evaluate and recognize metabolic dysfunction and progression of CKD, and the diet may have helped maintain and retard the progression of CKD. |
| first_indexed | 2024-03-10T05:16:34Z |
| format | Article |
| id | doaj.art-3b7f67872b454be5b0b7a22e079a8e05 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T05:16:34Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-3b7f67872b454be5b0b7a22e079a8e052023-11-23T00:21:28ZengMDPI AGMetabolites2218-19892021-11-01111178210.3390/metabo11110782Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease ProgressionMarcio Antonio Brunetto0Bruna Ruberti1Doris Pereira Halfen2Douglas Segalla Caragelasco3Thiago Henrique Annibale Vendramini4Vivian Pedrinelli5Henrique Tobaro Macedo6Juliana Toloi Jeremias7Cristiana Fonseca Ferreira Pontieri8Fernanda Maria Marins Ocampos9Luis Alberto Colnago10Marcia Mery Kogika11Pet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Jardim Elite, Pirassununga 13635-900, BrazilSmall Animal Internal Medicine Service, Veterinary Teaching Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, São Paulo 05508-270, BrazilVeterinary Nutrology Service, Veterinary Teaching Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, São Paulo 05508-270, BrazilSmall Animal Internal Medicine Service, Veterinary Teaching Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, São Paulo 05508-270, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Jardim Elite, Pirassununga 13635-900, BrazilVeterinary Nutrology Service, Veterinary Teaching Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, São Paulo 05508-270, BrazilPet Nutrology Research Center, Nutrition and Production Department, School of Veterinary Medicine and Animal Science, University of São Paulo, Jardim Elite, Pirassununga 13635-900, BrazilNutrition Development Center, Grandfood Industria e Comercio LTDA (Premier Pet<sup>®</sup>), Dourado, São Paulo 05508-270, BrazilNutrition Development Center, Grandfood Industria e Comercio LTDA (Premier Pet<sup>®</sup>), Dourado, São Paulo 05508-270, BrazilEmbrapa Instrumentação, R. 15 de Novembro, 1452, Centro, São Carlos 13560-970, BrazilEmbrapa Instrumentação, R. 15 de Novembro, 1452, Centro, São Carlos 13560-970, BrazilSmall Animal Internal Medicine Service, Veterinary Teaching Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, São Paulo 05508-270, BrazilChronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluate whether the diet would affect metabolites. Six dogs (5 females; 1 male; 7.47 ± 2.31 years old) with CKD stage 3 or 4 (IRIS) were included. CG consisted of 10 healthy female dogs (5.89 ± 2.57 years old) fed a maintenance diet. Serum metabolites were analyzed by <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectra. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to assess differences in metabolomic profiles between groups and before (CKD-T0) and after renal diet (CKD-T6). Data analysis was performed on SIMCA-P software. Dogs with CKD showed an altered metabolic profile with increased urea, creatinine, creatine, citrate, and lipids. Lactate, branched-chain amino acids (BCAAs), and glutamine were decreased in the CKD group. However, after 6 months of diet, the metabolite profiles of CKD-T0 and CKD-T6 were similar. Metabolomics profile may be useful to evaluate and recognize metabolic dysfunction and progression of CKD, and the diet may have helped maintain and retard the progression of CKD.https://www.mdpi.com/2218-1989/11/11/782renal dogsuremic toxinsmetabolic profilenutritionsurvival |
| spellingShingle | Marcio Antonio Brunetto Bruna Ruberti Doris Pereira Halfen Douglas Segalla Caragelasco Thiago Henrique Annibale Vendramini Vivian Pedrinelli Henrique Tobaro Macedo Juliana Toloi Jeremias Cristiana Fonseca Ferreira Pontieri Fernanda Maria Marins Ocampos Luis Alberto Colnago Marcia Mery Kogika Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression Metabolites renal dogs uremic toxins metabolic profile nutrition survival |
| title | Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression |
| title_full | Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression |
| title_fullStr | Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression |
| title_full_unstemmed | Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression |
| title_short | Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression |
| title_sort | healthy and chronic kidney disease ckd dogs have differences in serum metabolomics and renal diet may have slowed disease progression |
| topic | renal dogs uremic toxins metabolic profile nutrition survival |
| url | https://www.mdpi.com/2218-1989/11/11/782 |
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