Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model
In vitro adipose tissue models can be used to provide insight into fundamental aspects of adipose physiology. These systems may serve as replacements for animal models, which are often poor predictors of obesity and metabolic diseases in humans. Adipose tissue consists of a rich vasculature that is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/7/3/114 |
| _version_ | 1827705997987151872 |
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| author | Feipeng Yang Ronald N. Cohen Eric M. Brey |
| author_facet | Feipeng Yang Ronald N. Cohen Eric M. Brey |
| author_sort | Feipeng Yang |
| collection | DOAJ |
| description | In vitro adipose tissue models can be used to provide insight into fundamental aspects of adipose physiology. These systems may serve as replacements for animal models, which are often poor predictors of obesity and metabolic diseases in humans. Adipose tissue consists of a rich vasculature that is essential to its function. However, the study of endothelial cell–adipocyte interactions has been challenging due to differences in culture conditions required for the survival and function of each cell type. To address this issue, we performed an extensive evaluation of the cell culture media composition to identify the conditions optimal for the co-culture of endothelial cells and adipocytes. The effects of individual media factors on cell survival, proliferation, and differentiation were systematically explored. Several media factors were determined to disrupt the co-culture system. Optimized culture conditions were identified and used to generate a vascularized human adipose microtissue. An interconnected vascular network was established within an adipose micro-tissue, and the networks were anastomosed with perfused channels to form a functional network. In conclusion, media conditions were identified that enabled endothelial cell–adipocyte co-culture and were used to support the formation of a vascularized adipose tissue within a microfluidic device. |
| first_indexed | 2024-03-10T16:15:28Z |
| format | Article |
| id | doaj.art-3b8047ada99a4fb48a9db53035253dfc |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T16:15:28Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-3b8047ada99a4fb48a9db53035253dfc2023-11-20T14:03:31ZengMDPI AGBioengineering2306-53542020-09-017311410.3390/bioengineering7030114Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue ModelFeipeng Yang0Ronald N. Cohen1Eric M. Brey2Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USADepartment of Medicine, The University of Chicago, Chicago, IL 60637, USADepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, San Antonio, TX 78249, USAIn vitro adipose tissue models can be used to provide insight into fundamental aspects of adipose physiology. These systems may serve as replacements for animal models, which are often poor predictors of obesity and metabolic diseases in humans. Adipose tissue consists of a rich vasculature that is essential to its function. However, the study of endothelial cell–adipocyte interactions has been challenging due to differences in culture conditions required for the survival and function of each cell type. To address this issue, we performed an extensive evaluation of the cell culture media composition to identify the conditions optimal for the co-culture of endothelial cells and adipocytes. The effects of individual media factors on cell survival, proliferation, and differentiation were systematically explored. Several media factors were determined to disrupt the co-culture system. Optimized culture conditions were identified and used to generate a vascularized human adipose microtissue. An interconnected vascular network was established within an adipose micro-tissue, and the networks were anastomosed with perfused channels to form a functional network. In conclusion, media conditions were identified that enabled endothelial cell–adipocyte co-culture and were used to support the formation of a vascularized adipose tissue within a microfluidic device.https://www.mdpi.com/2306-5354/7/3/114vascularizationadipose tissueco-culturemicrofluidicsadipogenesis |
| spellingShingle | Feipeng Yang Ronald N. Cohen Eric M. Brey Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model Bioengineering vascularization adipose tissue co-culture microfluidics adipogenesis |
| title | Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model |
| title_full | Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model |
| title_fullStr | Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model |
| title_full_unstemmed | Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model |
| title_short | Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model |
| title_sort | optimization of co culture conditions for a human vascularized adipose tissue model |
| topic | vascularization adipose tissue co-culture microfluidics adipogenesis |
| url | https://www.mdpi.com/2306-5354/7/3/114 |
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