Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation.
<p>Abstract</p> <p>Background</p> <p>While dealing with mixed <it>in vitro</it> lymphocyte cultures one is faced with the problem of relative contributions of different populations to the activity being studied. This is especially true in the controversy rel...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2002-08-01
|
| Series: | BMC Immunology |
| Online Access: | http://www.biomedcentral.com/1471-2172/3/9 |
| _version_ | 1818163675724251136 |
|---|---|
| author | Narayanan PR Nirmala R |
| author_facet | Narayanan PR Nirmala R |
| author_sort | Narayanan PR |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>While dealing with mixed <it>in vitro</it> lymphocyte cultures one is faced with the problem of relative contributions of different populations to the activity being studied. This is especially true in the controversy relating to the contributions of lymphocyte sub-populations to the Lymphokine Activated Killer (LAK) phenomenon. Flowcytometry can be used to highlight relative contributions of lymphocyte subpopulations towards LAK activity without resorting to difficult purification strategies. We set up long-term <it>in vitro</it> lymphocyte cultures, stimulated them with cytokines IL-2/IL-12, recorded their phenotypic changes and cytotoxic activity against U-937 tumor targets.</p> <p>Results</p> <p>The results indicated that natural killer cells (NK) constituted the predominant proliferating cell population in the cytokine stimulatedcultures. Flowcytometric evidence revealed that CD56+ T cells contributed little to LAK activity against U937 target cells as compared to cells with NK phenotype which were predominantly responsible for spontaneous killing of the tumor targets. The two cytokines, IL-2 and IL-12, had an additive effect on cell proliferation and spontaneous cytotoxicity.</p> <p>Conclusion</p> <p>Flowcytometry can be used to rapidly delineate phenotypic changes in immune cells after stimulation and simultaneously correlate them with corresponding functional activity. This approach may find application as a initial screening tool for studying different types of cells in mixed cultures and their respective activities under stimulatory / inhibitory conditions.</p> |
| first_indexed | 2024-12-11T16:53:20Z |
| format | Article |
| id | doaj.art-3b80fef09e0b42cf8f01db26d80b1fb0 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-11T16:53:20Z |
| publishDate | 2002-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-3b80fef09e0b42cf8f01db26d80b1fb02022-12-22T00:58:03ZengBMCBMC Immunology1471-21722002-08-0131910.1186/1471-2172-3-9Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation.Narayanan PRNirmala R<p>Abstract</p> <p>Background</p> <p>While dealing with mixed <it>in vitro</it> lymphocyte cultures one is faced with the problem of relative contributions of different populations to the activity being studied. This is especially true in the controversy relating to the contributions of lymphocyte sub-populations to the Lymphokine Activated Killer (LAK) phenomenon. Flowcytometry can be used to highlight relative contributions of lymphocyte subpopulations towards LAK activity without resorting to difficult purification strategies. We set up long-term <it>in vitro</it> lymphocyte cultures, stimulated them with cytokines IL-2/IL-12, recorded their phenotypic changes and cytotoxic activity against U-937 tumor targets.</p> <p>Results</p> <p>The results indicated that natural killer cells (NK) constituted the predominant proliferating cell population in the cytokine stimulatedcultures. Flowcytometric evidence revealed that CD56+ T cells contributed little to LAK activity against U937 target cells as compared to cells with NK phenotype which were predominantly responsible for spontaneous killing of the tumor targets. The two cytokines, IL-2 and IL-12, had an additive effect on cell proliferation and spontaneous cytotoxicity.</p> <p>Conclusion</p> <p>Flowcytometry can be used to rapidly delineate phenotypic changes in immune cells after stimulation and simultaneously correlate them with corresponding functional activity. This approach may find application as a initial screening tool for studying different types of cells in mixed cultures and their respective activities under stimulatory / inhibitory conditions.</p>http://www.biomedcentral.com/1471-2172/3/9 |
| spellingShingle | Narayanan PR Nirmala R Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation. BMC Immunology |
| title | Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation. |
| title_full | Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation. |
| title_fullStr | Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation. |
| title_full_unstemmed | Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation. |
| title_short | Flowcytometry – A rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after <it>in vitro</it> stimulation. |
| title_sort | flowcytometry a rapid tool to correlate functional activities of human peripheral blood lymphocytes with their corresponding phenotypes after it in vitro it stimulation |
| url | http://www.biomedcentral.com/1471-2172/3/9 |
| work_keys_str_mv | AT narayananpr flowcytometryarapidtooltocorrelatefunctionalactivitiesofhumanperipheralbloodlymphocyteswiththeircorrespondingphenotypesafteritinvitroitstimulation AT nirmalar flowcytometryarapidtooltocorrelatefunctionalactivitiesofhumanperipheralbloodlymphocyteswiththeircorrespondingphenotypesafteritinvitroitstimulation |