OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-03-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/4/137 |
| _version_ | 1818007356615688192 |
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| author | Rhoda El Khoury Elodie Choque Anthony El Khoury Selma P. Snini Robbie Cairns Caroline Andriantsiferana Florence Mathieu |
| author_facet | Rhoda El Khoury Elodie Choque Anthony El Khoury Selma P. Snini Robbie Cairns Caroline Andriantsiferana Florence Mathieu |
| author_sort | Rhoda El Khoury |
| collection | DOAJ |
| description | Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination method using activated carbon fibers (ACFs). Actinobacterial strains were screened for their ability to reduce OTA in solid co-culture with A. carbonarius, which is the major OTA-producing species in European vineyards. Four strains showed a high affinity for removing OTA (67%–83%) with no significant effect on fungal growth (<20%). The mechanism of action was first studied by analyzing the expression of OTA cluster genes (acOTApks, acOTAnrps, acOTAhal) by RT-qPCR showing a drastic reduction in all genes (7–15 times). Second, the ability of these strains to degrade OTA was assessed in vitro on ISP2 solid medium supplemented with OTA (100 µg/L). Two strains reduced OTA to undetectable levels. As for the physical method, high adsorption rates were obtained for ACFs at 0.8 g/L with a 50% adsorption of OTA in red wine by AC15 and 52% in grape juice by AC20 within 24 h. These promising methods could be complementarily applied toward reducing OTA contamination in food chains, which promotes food safety and quality. |
| first_indexed | 2024-04-14T05:14:26Z |
| format | Article |
| id | doaj.art-3b8863f8b87d4652882219d192fda659 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T05:14:26Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3b8863f8b87d4652882219d192fda6592022-12-22T02:10:24ZengMDPI AGToxins2072-66512018-03-0110413710.3390/toxins10040137toxins10040137OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary ResultsRhoda El Khoury0Elodie Choque1Anthony El Khoury2Selma P. Snini3Robbie Cairns4Caroline Andriantsiferana5Florence Mathieu6Laboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXLaboratoire de Génie Chimique, Université de Toulouse, CNRS, Toulouse, France; Avenue de l’Agrobiopole-BP 32607-Auzeville-Tolosane 31326 CASTANET-TOLOSAN CEDEXOchratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium that contaminate food and feed raw materials. To reduce OTA contamination, we first tested in vitro, actinobacterial strains as potential biocontrol agents and afterward, through a physical decontamination method using activated carbon fibers (ACFs). Actinobacterial strains were screened for their ability to reduce OTA in solid co-culture with A. carbonarius, which is the major OTA-producing species in European vineyards. Four strains showed a high affinity for removing OTA (67%–83%) with no significant effect on fungal growth (<20%). The mechanism of action was first studied by analyzing the expression of OTA cluster genes (acOTApks, acOTAnrps, acOTAhal) by RT-qPCR showing a drastic reduction in all genes (7–15 times). Second, the ability of these strains to degrade OTA was assessed in vitro on ISP2 solid medium supplemented with OTA (100 µg/L). Two strains reduced OTA to undetectable levels. As for the physical method, high adsorption rates were obtained for ACFs at 0.8 g/L with a 50% adsorption of OTA in red wine by AC15 and 52% in grape juice by AC20 within 24 h. These promising methods could be complementarily applied toward reducing OTA contamination in food chains, which promotes food safety and quality.http://www.mdpi.com/2072-6651/10/4/137ochratoxin Abiocontroldetoxificationactinobacteriaactivated carbon fibers |
| spellingShingle | Rhoda El Khoury Elodie Choque Anthony El Khoury Selma P. Snini Robbie Cairns Caroline Andriantsiferana Florence Mathieu OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results Toxins ochratoxin A biocontrol detoxification actinobacteria activated carbon fibers |
| title | OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results |
| title_full | OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results |
| title_fullStr | OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results |
| title_full_unstemmed | OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results |
| title_short | OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results |
| title_sort | ota prevention and detoxification by actinobacterial strains and activated carbon fibers preliminary results |
| topic | ochratoxin A biocontrol detoxification actinobacteria activated carbon fibers |
| url | http://www.mdpi.com/2072-6651/10/4/137 |
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