A disialoganglioside of the globo-series from chicken skeletal muscle
We have isolated a disialoganglioside of the globo-series from chicken pectoral muscle. The compound was obtained by extraction followed by ion-exchange and silicic acid column chromatography and judged to be pure by thin-layer chromatography in three solvent systems. The structure of the gangliosid...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1991-03-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520420735 |
| _version_ | 1818671915223482368 |
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| author | S Dasgupta JL Chien EL Hogan H van Halbeek |
| author_facet | S Dasgupta JL Chien EL Hogan H van Halbeek |
| author_sort | S Dasgupta |
| collection | DOAJ |
| description | We have isolated a disialoganglioside of the globo-series from chicken pectoral muscle. The compound was obtained by extraction followed by ion-exchange and silicic acid column chromatography and judged to be pure by thin-layer chromatography in three solvent systems. The structure of the ganglioside was determined by carbohydrate and ceramide composition analysis, sequential exoglycosidase digestion, methylation analysis, and 500-MHz 1H-NMR spectroscopy to be: (formula; see text) Analysis of the ceramide moiety indicated d18:1 sphingosine as the long-chain base, and C16:0, C18:0, C18:1, and C20:0 as the prevalent fatty acids. This glycolipid is only the second ganglioside of the globo-series, and the first disialo member of the series, found in chicken muscle. |
| first_indexed | 2024-12-17T07:31:35Z |
| format | Article |
| id | doaj.art-3b8d2e45288f47e98aa1cd71183517a1 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T07:31:35Z |
| publishDate | 1991-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-3b8d2e45288f47e98aa1cd71183517a12022-12-21T21:58:29ZengElsevierJournal of Lipid Research0022-22751991-03-01323499506A disialoganglioside of the globo-series from chicken skeletal muscleS Dasgupta0JL Chien1EL Hogan2H van Halbeek3Department of Neurology, Medical University of South Carolina, Charleston.Department of Neurology, Medical University of South Carolina, Charleston.Department of Neurology, Medical University of South Carolina, Charleston.Department of Neurology, Medical University of South Carolina, Charleston.We have isolated a disialoganglioside of the globo-series from chicken pectoral muscle. The compound was obtained by extraction followed by ion-exchange and silicic acid column chromatography and judged to be pure by thin-layer chromatography in three solvent systems. The structure of the ganglioside was determined by carbohydrate and ceramide composition analysis, sequential exoglycosidase digestion, methylation analysis, and 500-MHz 1H-NMR spectroscopy to be: (formula; see text) Analysis of the ceramide moiety indicated d18:1 sphingosine as the long-chain base, and C16:0, C18:0, C18:1, and C20:0 as the prevalent fatty acids. This glycolipid is only the second ganglioside of the globo-series, and the first disialo member of the series, found in chicken muscle.http://www.sciencedirect.com/science/article/pii/S0022227520420735 |
| spellingShingle | S Dasgupta JL Chien EL Hogan H van Halbeek A disialoganglioside of the globo-series from chicken skeletal muscle Journal of Lipid Research |
| title | A disialoganglioside of the globo-series from chicken skeletal muscle |
| title_full | A disialoganglioside of the globo-series from chicken skeletal muscle |
| title_fullStr | A disialoganglioside of the globo-series from chicken skeletal muscle |
| title_full_unstemmed | A disialoganglioside of the globo-series from chicken skeletal muscle |
| title_short | A disialoganglioside of the globo-series from chicken skeletal muscle |
| title_sort | disialoganglioside of the globo series from chicken skeletal muscle |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520420735 |
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