Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio
Abstract The biocontrol potential of four Pochonia chlamydosporia var. chlamydosporia isolates on the root-knot nematode, Meloidogyne javanica was investigated on pistachio plants in a greenhouse experiment. Isolates were applied at 10,000, 5000 and 1000 chlamydospores per gram of soil; the latter t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-05-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41938-018-0047-y |
| _version_ | 1818616039185842176 |
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| author | M. Ebadi S. Fatemy H. Riahi |
| author_facet | M. Ebadi S. Fatemy H. Riahi |
| author_sort | M. Ebadi |
| collection | DOAJ |
| description | Abstract The biocontrol potential of four Pochonia chlamydosporia var. chlamydosporia isolates on the root-knot nematode, Meloidogyne javanica was investigated on pistachio plants in a greenhouse experiment. Isolates were applied at 10,000, 5000 and 1000 chlamydospores per gram of soil; the latter two densities were being used for strain Pcc60 only and designated as PccB and PccA, respectively. Plants were inoculated with a suspension containing 3000 eggs of M. javanica. Nematode reproduction was reduced to 57% by Pcc20 and 36% by Pcc10 and Pcc60C after 4 months. Pcc20 was the most promising isolate as the final egg population of nematodes was reduced by nearly 61 and 36% by Pcc10 and Pcc60C, respectively. All strains infected eggs on the roots to varying degrees. Pcc20 was the most effective colonizer of all the isolates, infecting 37% of the eggs, while Pcc10 and Pcc60C as the second best colonizers caused an average infection rate of 17.5%. The potential of the fungus was reduced at lower densities of spore inoculum. Semi-selective medium was used to re-isolate the fungi from soil and rhizosphere but was not able to detect Pcc60A at 1000 cfu/g soil, despite the fact that 3% of the eggs were parasitized. The efficiency of the different isolates in controlling nematode reproduction parameters and their relationships in terms of survival and abundance is discussed further. |
| first_indexed | 2024-12-16T16:43:27Z |
| format | Article |
| id | doaj.art-3b92a8f0c9e346b899dc25f95aad25f9 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-16T16:43:27Z |
| publishDate | 2018-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-3b92a8f0c9e346b899dc25f95aad25f92022-12-21T22:24:14ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422018-05-012811610.1186/s41938-018-0047-yBiocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachioM. Ebadi0S. Fatemy1H. Riahi2Faculty of Basic Sciences, Azarbaijan Shahid Madani UniversityNematology Department, Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension Organization, AREEOTechnical and Engineering Campus of Shahid Beheshti UniversityAbstract The biocontrol potential of four Pochonia chlamydosporia var. chlamydosporia isolates on the root-knot nematode, Meloidogyne javanica was investigated on pistachio plants in a greenhouse experiment. Isolates were applied at 10,000, 5000 and 1000 chlamydospores per gram of soil; the latter two densities were being used for strain Pcc60 only and designated as PccB and PccA, respectively. Plants were inoculated with a suspension containing 3000 eggs of M. javanica. Nematode reproduction was reduced to 57% by Pcc20 and 36% by Pcc10 and Pcc60C after 4 months. Pcc20 was the most promising isolate as the final egg population of nematodes was reduced by nearly 61 and 36% by Pcc10 and Pcc60C, respectively. All strains infected eggs on the roots to varying degrees. Pcc20 was the most effective colonizer of all the isolates, infecting 37% of the eggs, while Pcc10 and Pcc60C as the second best colonizers caused an average infection rate of 17.5%. The potential of the fungus was reduced at lower densities of spore inoculum. Semi-selective medium was used to re-isolate the fungi from soil and rhizosphere but was not able to detect Pcc60A at 1000 cfu/g soil, despite the fact that 3% of the eggs were parasitized. The efficiency of the different isolates in controlling nematode reproduction parameters and their relationships in terms of survival and abundance is discussed further.http://link.springer.com/article/10.1186/s41938-018-0047-yBiological controlPistacia veraRoot-knot nematodeMeloidogyne javanicaSurvivalPochonia chlamydosporia var. chlamydosporia |
| spellingShingle | M. Ebadi S. Fatemy H. Riahi Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio Egyptian Journal of Biological Pest Control Biological control Pistacia vera Root-knot nematode Meloidogyne javanica Survival Pochonia chlamydosporia var. chlamydosporia |
| title | Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio |
| title_full | Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio |
| title_fullStr | Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio |
| title_full_unstemmed | Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio |
| title_short | Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio |
| title_sort | biocontrol potential of pochonia chlamydosporia var chlamydosporia isolates against meloidogyne javanica on pistachio |
| topic | Biological control Pistacia vera Root-knot nematode Meloidogyne javanica Survival Pochonia chlamydosporia var. chlamydosporia |
| url | http://link.springer.com/article/10.1186/s41938-018-0047-y |
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