Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301
Cowpea is the most important food legume in West and Central Africa and a valuable economic commodity in the region. Among the major biotic constraints to cowpea production are root parasitic weeds of which <i>Alectra vogelii</i> (Benth.) is of increasing importance. The cowpea landrace...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/11/2654 |
| _version_ | 1797469467339390976 |
|---|---|
| author | Erik W. Ohlson Michael P. Timko |
| author_facet | Erik W. Ohlson Michael P. Timko |
| author_sort | Erik W. Ohlson |
| collection | DOAJ |
| description | Cowpea is the most important food legume in West and Central Africa and a valuable economic commodity in the region. Among the major biotic constraints to cowpea production are root parasitic weeds of which <i>Alectra vogelii</i> (Benth.) is of increasing importance. The cowpea landrace B301 was previously identified as a source of <i>Alectra</i> resistance, but neither the genes nor genomic loci conferring this resistance have been mapped. Therefore, to map and identify genetic markers linked to <i>Alectra</i> resistance for use in the molecular improvement of cowpea, we developed an F<sub>2</sub> population from a cross of the susceptible variety 524B with B301. The population was phenotyped for resistance to <i>A. vogelii</i> and genotyped with a cowpea high density single nucleotide polymorphism (SNP) microarray. Putative resistance loci were mapped in F<sub>2</sub> populations by categorical trait–multiple interval mapping and validated by selective genotyping. Selective genotyping indicated that the resistance loci on Vu04 (<i>Rav1</i>) and Vu11 (<i>Rav2</i>) were significantly associated with resistance (<i>p</i> ≤ 0.01). Using marker assisted backcrossing, the two resistance loci were introgressed independently into the susceptible 524B genetic background. Phenotyping and genotyping of the segregating backcross families delineated <i>Rav1</i> to a 10 cM on chromosome 4 and <i>Rav2</i> to a 6.7 cM interval in chromosome 11. These two loci are desirable for breeding <i>Alectra</i> resistant cowpea varieties due to their simple inheritance and ability to independently confer complete immunity to the parasite. |
| first_indexed | 2024-03-09T19:20:53Z |
| format | Article |
| id | doaj.art-3b936c7774394382a882dcf26b2b9f8c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T19:20:53Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-3b936c7774394382a882dcf26b2b9f8c2023-11-24T03:19:57ZengMDPI AGAgronomy2073-43952022-10-011211265410.3390/agronomy12112654Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301Erik W. Ohlson0Michael P. Timko1Department of Biology, University of Virginia, Charlottesville, VA 22904, USADepartment of Biology, University of Virginia, Charlottesville, VA 22904, USACowpea is the most important food legume in West and Central Africa and a valuable economic commodity in the region. Among the major biotic constraints to cowpea production are root parasitic weeds of which <i>Alectra vogelii</i> (Benth.) is of increasing importance. The cowpea landrace B301 was previously identified as a source of <i>Alectra</i> resistance, but neither the genes nor genomic loci conferring this resistance have been mapped. Therefore, to map and identify genetic markers linked to <i>Alectra</i> resistance for use in the molecular improvement of cowpea, we developed an F<sub>2</sub> population from a cross of the susceptible variety 524B with B301. The population was phenotyped for resistance to <i>A. vogelii</i> and genotyped with a cowpea high density single nucleotide polymorphism (SNP) microarray. Putative resistance loci were mapped in F<sub>2</sub> populations by categorical trait–multiple interval mapping and validated by selective genotyping. Selective genotyping indicated that the resistance loci on Vu04 (<i>Rav1</i>) and Vu11 (<i>Rav2</i>) were significantly associated with resistance (<i>p</i> ≤ 0.01). Using marker assisted backcrossing, the two resistance loci were introgressed independently into the susceptible 524B genetic background. Phenotyping and genotyping of the segregating backcross families delineated <i>Rav1</i> to a 10 cM on chromosome 4 and <i>Rav2</i> to a 6.7 cM interval in chromosome 11. These two loci are desirable for breeding <i>Alectra</i> resistant cowpea varieties due to their simple inheritance and ability to independently confer complete immunity to the parasite.https://www.mdpi.com/2073-4395/12/11/2654cowpea<i>Alectra vogelii</i>plant parasitismcrop geneticsQTL mapping |
| spellingShingle | Erik W. Ohlson Michael P. Timko Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301 Agronomy cowpea <i>Alectra vogelii</i> plant parasitism crop genetics QTL mapping |
| title | Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301 |
| title_full | Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301 |
| title_fullStr | Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301 |
| title_full_unstemmed | Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301 |
| title_short | Mapping and Validation of <i>Alectra vogelii</i> Resistance in the Cowpea Landrace B301 |
| title_sort | mapping and validation of i alectra vogelii i resistance in the cowpea landrace b301 |
| topic | cowpea <i>Alectra vogelii</i> plant parasitism crop genetics QTL mapping |
| url | https://www.mdpi.com/2073-4395/12/11/2654 |
| work_keys_str_mv | AT erikwohlson mappingandvalidationofialectravogeliiiresistanceinthecowpealandraceb301 AT michaelptimko mappingandvalidationofialectravogeliiiresistanceinthecowpealandraceb301 |