Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling
Polysaccharides are the essential active constituents of Radix Pueraria lobata. Numerous studies have shown that polysaccharides can play important roles in ulcerative colitis (UC). This study aimed to determine the protective effects of Radix Pueraria lobata polysaccharide (PPL) against UC. The pha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001147 |
| _version_ | 1797837082101547008 |
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| author | Zhuang Zhang Yushun Cui Hui Ouyang Weifeng Zhu Yulin Feng Min Yao Shilin Yang |
| author_facet | Zhuang Zhang Yushun Cui Hui Ouyang Weifeng Zhu Yulin Feng Min Yao Shilin Yang |
| author_sort | Zhuang Zhang |
| collection | DOAJ |
| description | Polysaccharides are the essential active constituents of Radix Pueraria lobata. Numerous studies have shown that polysaccharides can play important roles in ulcerative colitis (UC). This study aimed to determine the protective effects of Radix Pueraria lobata polysaccharide (PPL) against UC. The pharmacological effects of PPL on the dextran sulfate sodium (DSS)-induced UC mice, changes in gut microbiota and metabolism, and DSS-induced Caco-2 cell injury were assessed in this study. Both the PPL inhibited the DSS-induced inflammation both in vivo and in vitro. Mechanistically, PPL inhibited inflammation by decreasing the activation of p-PI3K. Knocking down the PI3K gene resulted in abolishing the inhibitory effects of PPL on mouses and Caco-2 cells. The study concluded that the effects of PPL were completely dependent on PI3K. |
| first_indexed | 2024-04-09T15:20:12Z |
| format | Article |
| id | doaj.art-3b93c26d3b4a468bb442520c180dcdd9 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-09T15:20:12Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-3b93c26d3b4a468bb442520c180dcdd92023-04-29T14:48:23ZengElsevierJournal of Functional Foods1756-46462023-05-01104105514Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signalingZhuang Zhang0Yushun Cui1Hui Ouyang2Weifeng Zhu3Yulin Feng4Min Yao5Shilin Yang6National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, No. 56 Yangming Road, Nanchang 330006, PR ChinaNational Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, No. 56 Yangming Road, Nanchang 330006, PR ChinaNational Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, No. 56 Yangming Road, Nanchang 330006, PR China; Corresponding authors.Key Laboratory of Modern Preparation of Chinese Medicine of Jiangxi University of Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China; Corresponding authors.National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, No. 56 Yangming Road, Nanchang 330006, PR China; Corresponding authors.Jiangxi Institute of Drug Inspection and Testing, State Drug Administration Key Laboratory of Quality Evaluation of Proprietary Chinese Medicine, Nanchang 330029, PR ChinaNational Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, No. 56 Yangming Road, Nanchang 330006, PR ChinaPolysaccharides are the essential active constituents of Radix Pueraria lobata. Numerous studies have shown that polysaccharides can play important roles in ulcerative colitis (UC). This study aimed to determine the protective effects of Radix Pueraria lobata polysaccharide (PPL) against UC. The pharmacological effects of PPL on the dextran sulfate sodium (DSS)-induced UC mice, changes in gut microbiota and metabolism, and DSS-induced Caco-2 cell injury were assessed in this study. Both the PPL inhibited the DSS-induced inflammation both in vivo and in vitro. Mechanistically, PPL inhibited inflammation by decreasing the activation of p-PI3K. Knocking down the PI3K gene resulted in abolishing the inhibitory effects of PPL on mouses and Caco-2 cells. The study concluded that the effects of PPL were completely dependent on PI3K.http://www.sciencedirect.com/science/article/pii/S1756464623001147Radix Puerariae lobata polysaccharideUlcerative colitisGut microbiotaMetabonomicsKnockdownPI3K |
| spellingShingle | Zhuang Zhang Yushun Cui Hui Ouyang Weifeng Zhu Yulin Feng Min Yao Shilin Yang Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling Journal of Functional Foods Radix Puerariae lobata polysaccharide Ulcerative colitis Gut microbiota Metabonomics Knockdown PI3K |
| title | Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling |
| title_full | Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling |
| title_fullStr | Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling |
| title_full_unstemmed | Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling |
| title_short | Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling |
| title_sort | radix pueraria lobata polysaccharide relieved dss induced ulcerative colitis through modulating pi3k signaling |
| topic | Radix Puerariae lobata polysaccharide Ulcerative colitis Gut microbiota Metabonomics Knockdown PI3K |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001147 |
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