Label-Free Cross-Priming Amplification Coupled With Endonuclease Restriction and Nanoparticles-Based Biosensor for Simultaneous Detection of Nucleic Acids and Prevention of Carryover Contamination

Label-Free Cross-Priming Amplification Coupled With Endonuclease Restriction and Nanoparticles-Based Biosensor for Simultaneous Detection of Nucleic Acids and Prevention of Carryover Contamination

Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonuclease restriction for simultaneous detection of nucleic acids and elimination of carryover contamination. Reaction mixtures were detected in a nanoparticle-based lateral flow biosensor (LFB). The assay ex...

Full description

Bibliographic Details
Main Authors: Yi Wang, Lin Sun, Jie-qiong Li, Ze-ming Wang, Wei-wei Jiao, Jing Xiao, Chen Shen, Fang Xu, Hui Qi, Yong-hong Wang, Ya-jie Guo, A-dong Shen
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Chemistry
Subjects:
cross-priming amplification
endonuclease restriction
lateral flow biosensor
S. aureus
limit of detection
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2019.00322/full

Internet

https://www.frontiersin.org/article/10.3389/fchem.2019.00322/full

Similar Items