Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China
Abstract Sediment bacterial communities play a critical role in biogeochemical cycling in alpine lake ecosystems. However, little is known about the sediment microbial communities in these lakes. In this study, the bacterial community composition (BCC) and their relationships with environmental fact...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02793-1 |
| _version_ | 1797865392025108480 |
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| author | Keqiang Shao Lei Zhang Tunasheng Ba Jianying Chao Guang Gao |
| author_facet | Keqiang Shao Lei Zhang Tunasheng Ba Jianying Chao Guang Gao |
| author_sort | Keqiang Shao |
| collection | DOAJ |
| description | Abstract Sediment bacterial communities play a critical role in biogeochemical cycling in alpine lake ecosystems. However, little is known about the sediment microbial communities in these lakes. In this study, the bacterial community composition (BCC) and their relationships with environmental factors of the sediment in Sayram Lake, the largest alpine and cold-water inland lake, China was analyzed using Illumina MiSeq sequencing. In total, we obtained 618,271 high quality sequences. The results showed that the bacterial communities with 30 phyla and 546 genera, were spread out among the 5 furface sediment samples, respectively. The communities were dominated by Proteobacteria, Acidobacteria, Planctomycetes, Gemmatimonadetes, Chloroflexi, Actinobacteria, Verrucomicrobia and Bacteroidetes, accounting for 48.15 ± 8.10%, 11.23 ± 3.10%, 8.42 ± 2.15%, 8.37 ± 2.26%, 7.40 ± 3.05%, 5.62 ± 1.25%, 4.18 ± 2.12% and 2.24 ± 1.10% of the total reads, respectively. At the genus level, the communities were dominated by Aquabacterium, Pseudomonas, Woeseia, MND1, Ignavibacterium and Truepera, accounting for 7.89% ± 8.24%, 2.32% ± 1.05%, 2.14% ± 0.94%, 2% ± 1.22%, 0.94% ± 0.14% and 0.80% ± 0.14% of the total reads, respectively. Statistical analyses showed the similarity of the sediment bacterial communities at our field sites was considerably low, far below 35%, and total organic carbon (TOC) was the dominant environmental factor affecting the spatial changes of BCC in the sediment. Thus, this study greatly improving our understanding of the microbial ecology of alpine lake in the arid and semi-arid ecosystems today so seriously threatened. |
| first_indexed | 2024-04-09T23:08:17Z |
| format | Article |
| id | doaj.art-3b9d33ce80cf4391afe7e63c95e1a680 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-09T23:08:17Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-3b9d33ce80cf4391afe7e63c95e1a6802023-03-22T10:34:27ZengBMCBMC Microbiology1471-21802023-02-012311810.1186/s12866-023-02793-1Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of ChinaKeqiang Shao0Lei Zhang1Tunasheng Ba2Jianying Chao3Guang Gao4School of Civil Engineering and Architecture, Chuzhou UniversitySchool of Civil Engineering and Architecture, Chuzhou UniversityEnvironmental Monitoring Station of Bayingolin Mongolia Autonomous PrefectureNanjing Institute of Environmental Sciences, Ministry of Ecology and EnvironmentNanjing Institute of Geography and Limnology, Chinese Academy of SciencesAbstract Sediment bacterial communities play a critical role in biogeochemical cycling in alpine lake ecosystems. However, little is known about the sediment microbial communities in these lakes. In this study, the bacterial community composition (BCC) and their relationships with environmental factors of the sediment in Sayram Lake, the largest alpine and cold-water inland lake, China was analyzed using Illumina MiSeq sequencing. In total, we obtained 618,271 high quality sequences. The results showed that the bacterial communities with 30 phyla and 546 genera, were spread out among the 5 furface sediment samples, respectively. The communities were dominated by Proteobacteria, Acidobacteria, Planctomycetes, Gemmatimonadetes, Chloroflexi, Actinobacteria, Verrucomicrobia and Bacteroidetes, accounting for 48.15 ± 8.10%, 11.23 ± 3.10%, 8.42 ± 2.15%, 8.37 ± 2.26%, 7.40 ± 3.05%, 5.62 ± 1.25%, 4.18 ± 2.12% and 2.24 ± 1.10% of the total reads, respectively. At the genus level, the communities were dominated by Aquabacterium, Pseudomonas, Woeseia, MND1, Ignavibacterium and Truepera, accounting for 7.89% ± 8.24%, 2.32% ± 1.05%, 2.14% ± 0.94%, 2% ± 1.22%, 0.94% ± 0.14% and 0.80% ± 0.14% of the total reads, respectively. Statistical analyses showed the similarity of the sediment bacterial communities at our field sites was considerably low, far below 35%, and total organic carbon (TOC) was the dominant environmental factor affecting the spatial changes of BCC in the sediment. Thus, this study greatly improving our understanding of the microbial ecology of alpine lake in the arid and semi-arid ecosystems today so seriously threatened.https://doi.org/10.1186/s12866-023-02793-1Sayram LakeSurface sedimentBCCTOCIllumina Miseq sequencing |
| spellingShingle | Keqiang Shao Lei Zhang Tunasheng Ba Jianying Chao Guang Gao Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China BMC Microbiology Sayram Lake Surface sediment BCC TOC Illumina Miseq sequencing |
| title | Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China |
| title_full | Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China |
| title_fullStr | Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China |
| title_full_unstemmed | Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China |
| title_short | Bacterial community composition of the sediment in Sayram Lake, an alpine lake in the arid northwest of China |
| title_sort | bacterial community composition of the sediment in sayram lake an alpine lake in the arid northwest of china |
| topic | Sayram Lake Surface sediment BCC TOC Illumina Miseq sequencing |
| url | https://doi.org/10.1186/s12866-023-02793-1 |
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