Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump
Aim: Heavy metal pollution is serious in China, and abscisic acid (ABA) is an important stress hormone. How it regulates plant tolerance to cadmium remains unclear, so we aimed to explore the molecular mechanism responsible for enhanced cadmium resistance in Arabidopsis wild-type and mutant plants a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01892/full |
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| author | Tao Wang Tao Wang Yingpeng Hua Yingpeng Hua Moxian Chen Jianhua Zhang Chunyun Guan Zhenhua Zhang Zhenhua Zhang |
| author_facet | Tao Wang Tao Wang Yingpeng Hua Yingpeng Hua Moxian Chen Jianhua Zhang Chunyun Guan Zhenhua Zhang Zhenhua Zhang |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | Aim: Heavy metal pollution is serious in China, and abscisic acid (ABA) is an important stress hormone. How it regulates plant tolerance to cadmium remains unclear, so we aimed to explore the molecular mechanism responsible for enhanced cadmium resistance in Arabidopsis wild-type and mutant plants and Brassica napus seedlings.Methods: Arabidopsis/B. napus were cultured hydroponically for 28/15 days and then treated with 20/10 μM Cd/Cd+ABA (5 μM) for 3/4 days. Chlorophyll degradation rate, SPAD values, proline, MDA, ABA, NO3−, and Cd concentrations were measured in root vacuoles and protoplasts; root to shoot NO3− and Cd concentration ratios were determined and NRT1.5-, NRT1.8-, BnNRT1.5-, and BnNRT1.8-related gene expression was studied.Results: Cytoplasmic ABA levels in root cells of bglu10 and bglu18 Arabidopsis mutants were significantly lower than those in the wild-type, apparently making the latter more resistant to Cd. NO3− long-distance transporter NRT1.5 responded to ABA signaling by downregulating its own expression, while NRT1.8 did not respond. Concomitantly, proton pump activity in wild-type plants was higher than in the bglu10 and bglu18 mutants; thus, more NO3− and Cd accumulated in the vacuoles of wild-type root cells. ABA application inhibited Cd absorption by B. napus. BnNRT1.5 responded to exogenous ABA signal by downregulating its own expression, while the lack of response by BnNRT1.8 resulted in increased amount of NO3− accumulating in the roots to participate in the anti-cadmium reaction.Conclusion:NRT1.5 responds to the ABA signal to inhibit its own expression, whereas unresponsiveness of NRT1.8 causes accumulation of NO3− in the roots; thus, enhancing Cd resistance. In Arabidopsis, because of proton pump action, more NO3− and Cd accumulate in the vacuoles of Arabidopsis root cells, thereby reducing damage by Cd toxicity. However, in B. napus, the addition of exogenous ABA inhibited Cd absorption. Our data provide a sound basis to the theoretical molecular mechanism involved in hormone signaling during response of plants to heavy metal stress. |
| first_indexed | 2024-12-16T07:52:33Z |
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| id | doaj.art-3b9d7eb547cc4ceaa0d69263697150b9 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T07:52:33Z |
| publishDate | 2018-12-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-3b9d7eb547cc4ceaa0d69263697150b92022-12-21T22:38:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-12-01910.3389/fpls.2018.01892421545Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton PumpTao Wang0Tao Wang1Yingpeng Hua2Yingpeng Hua3Moxian Chen4Jianhua Zhang5Chunyun Guan6Zhenhua Zhang7Zhenhua Zhang8Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Provincial Key Laboratory of Nutrition in Common University, National Engineering Laboratory on Soil and Fertilizer Resources Efficient Utilization, Changsha, ChinaSouthern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Provincial Key Laboratory of Nutrition in Common University, National Engineering Laboratory on Soil and Fertilizer Resources Efficient Utilization, Changsha, ChinaDepartment of Biology, Hong Kong Baptist University and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Biology, Hong Kong Baptist University, Hong Kong, ChinaNational Center of Oilseed Crops Improvement, Hunan Branch, Changsha, ChinaSouthern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Provincial Key Laboratory of Nutrition in Common University, National Engineering Laboratory on Soil and Fertilizer Resources Efficient Utilization, Changsha, ChinaAim: Heavy metal pollution is serious in China, and abscisic acid (ABA) is an important stress hormone. How it regulates plant tolerance to cadmium remains unclear, so we aimed to explore the molecular mechanism responsible for enhanced cadmium resistance in Arabidopsis wild-type and mutant plants and Brassica napus seedlings.Methods: Arabidopsis/B. napus were cultured hydroponically for 28/15 days and then treated with 20/10 μM Cd/Cd+ABA (5 μM) for 3/4 days. Chlorophyll degradation rate, SPAD values, proline, MDA, ABA, NO3−, and Cd concentrations were measured in root vacuoles and protoplasts; root to shoot NO3− and Cd concentration ratios were determined and NRT1.5-, NRT1.8-, BnNRT1.5-, and BnNRT1.8-related gene expression was studied.Results: Cytoplasmic ABA levels in root cells of bglu10 and bglu18 Arabidopsis mutants were significantly lower than those in the wild-type, apparently making the latter more resistant to Cd. NO3− long-distance transporter NRT1.5 responded to ABA signaling by downregulating its own expression, while NRT1.8 did not respond. Concomitantly, proton pump activity in wild-type plants was higher than in the bglu10 and bglu18 mutants; thus, more NO3− and Cd accumulated in the vacuoles of wild-type root cells. ABA application inhibited Cd absorption by B. napus. BnNRT1.5 responded to exogenous ABA signal by downregulating its own expression, while the lack of response by BnNRT1.8 resulted in increased amount of NO3− accumulating in the roots to participate in the anti-cadmium reaction.Conclusion:NRT1.5 responds to the ABA signal to inhibit its own expression, whereas unresponsiveness of NRT1.8 causes accumulation of NO3− in the roots; thus, enhancing Cd resistance. In Arabidopsis, because of proton pump action, more NO3− and Cd accumulate in the vacuoles of Arabidopsis root cells, thereby reducing damage by Cd toxicity. However, in B. napus, the addition of exogenous ABA inhibited Cd absorption. Our data provide a sound basis to the theoretical molecular mechanism involved in hormone signaling during response of plants to heavy metal stress.https://www.frontiersin.org/article/10.3389/fpls.2018.01892/fullABA signalingNRT1.5NRT1.8NO3−Cd stressproton pump activity |
| spellingShingle | Tao Wang Tao Wang Yingpeng Hua Yingpeng Hua Moxian Chen Jianhua Zhang Chunyun Guan Zhenhua Zhang Zhenhua Zhang Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump Frontiers in Plant Science ABA signaling NRT1.5 NRT1.8 NO3− Cd stress proton pump activity |
| title | Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump |
| title_full | Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump |
| title_fullStr | Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump |
| title_full_unstemmed | Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump |
| title_short | Mechanism Enhancing Arabidopsis Resistance to Cadmium: The Role of NRT1.5 and Proton Pump |
| title_sort | mechanism enhancing arabidopsis resistance to cadmium the role of nrt1 5 and proton pump |
| topic | ABA signaling NRT1.5 NRT1.8 NO3− Cd stress proton pump activity |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01892/full |
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