Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research
Human pluripotent stem cells have been employed in generating organoids, yet their immaturity compared to fetal organs and the limited induction of all constituent cell types remain challenges. Porcine fetal progenitor cells have emerged as promising candidates for co-culturing with human progenitor...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2073-4409/13/3/228 |
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| author | Kenji Matsui Hidekazu Sekine Jun Ishikawa Shin Enosawa Naoto Matsumoto Yuka Inage Yoshitaka Kinoshita Keita Morimoto Shutaro Yamamoto Nagisa Koda Shuichiro Yamanaka Takashi Yokoo Eiji Kobayashi |
| author_facet | Kenji Matsui Hidekazu Sekine Jun Ishikawa Shin Enosawa Naoto Matsumoto Yuka Inage Yoshitaka Kinoshita Keita Morimoto Shutaro Yamamoto Nagisa Koda Shuichiro Yamanaka Takashi Yokoo Eiji Kobayashi |
| author_sort | Kenji Matsui |
| collection | DOAJ |
| description | Human pluripotent stem cells have been employed in generating organoids, yet their immaturity compared to fetal organs and the limited induction of all constituent cell types remain challenges. Porcine fetal progenitor cells have emerged as promising candidates for co-culturing with human progenitor cells in regeneration and xenotransplantation research. This study focused on identifying proper preservation methods for porcine fetal kidneys, hearts, and livers, aiming to optimize their potential as cell sources. Extracted from fetal microminiature pigs, these organs were dissociated before and after cryopreservation–thawing, with subsequent cell quality evaluations. Kidney cells, dissociated and aggregated after vitrification in a whole-organ form, were successfully differentiated into glomeruli and tubules in vivo. In contrast, freezing hearts and livers before dissociation yielded suboptimal results. Heart cells, frozen after dissociation, exhibited pulsating heart muscle cells similar to non-frozen hearts. As for liver cells, we developed a direct tissue perfusion technique and successfully obtained highly viable liver parenchymal cells. Freezing dissociated liver cells, although inferior to their non-frozen counterparts, maintained the ability for colony formation. The findings of this study provide valuable insights into suitable preservation methods for porcine fetal cells from kidneys, hearts, and livers, contributing to the advancement of regeneration and xenotransplantation research. |
| first_indexed | 2024-03-08T03:59:25Z |
| format | Article |
| id | doaj.art-3b9e081eaac247c898041b7ef568ce0b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-08T03:59:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3b9e081eaac247c898041b7ef568ce0b2024-02-09T15:09:38ZengMDPI AGCells2073-44092024-01-0113322810.3390/cells13030228Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine ResearchKenji Matsui0Hidekazu Sekine1Jun Ishikawa2Shin Enosawa3Naoto Matsumoto4Yuka Inage5Yoshitaka Kinoshita6Keita Morimoto7Shutaro Yamamoto8Nagisa Koda9Shuichiro Yamanaka10Takashi Yokoo11Eiji Kobayashi12Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo 162-0056, JapanDivision for Advanced Medical Sciences, National Center for Child Health and Development, Tokyo 157-8535, JapanDivision for Advanced Medical Sciences, National Center for Child Health and Development, Tokyo 157-8535, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDepartment of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanHuman pluripotent stem cells have been employed in generating organoids, yet their immaturity compared to fetal organs and the limited induction of all constituent cell types remain challenges. Porcine fetal progenitor cells have emerged as promising candidates for co-culturing with human progenitor cells in regeneration and xenotransplantation research. This study focused on identifying proper preservation methods for porcine fetal kidneys, hearts, and livers, aiming to optimize their potential as cell sources. Extracted from fetal microminiature pigs, these organs were dissociated before and after cryopreservation–thawing, with subsequent cell quality evaluations. Kidney cells, dissociated and aggregated after vitrification in a whole-organ form, were successfully differentiated into glomeruli and tubules in vivo. In contrast, freezing hearts and livers before dissociation yielded suboptimal results. Heart cells, frozen after dissociation, exhibited pulsating heart muscle cells similar to non-frozen hearts. As for liver cells, we developed a direct tissue perfusion technique and successfully obtained highly viable liver parenchymal cells. Freezing dissociated liver cells, although inferior to their non-frozen counterparts, maintained the ability for colony formation. The findings of this study provide valuable insights into suitable preservation methods for porcine fetal cells from kidneys, hearts, and livers, contributing to the advancement of regeneration and xenotransplantation research.https://www.mdpi.com/2073-4409/13/3/228fetal pigcell sourceorgan preservationcryopreservationvitrificationxenotransplantation |
| spellingShingle | Kenji Matsui Hidekazu Sekine Jun Ishikawa Shin Enosawa Naoto Matsumoto Yuka Inage Yoshitaka Kinoshita Keita Morimoto Shutaro Yamamoto Nagisa Koda Shuichiro Yamanaka Takashi Yokoo Eiji Kobayashi Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research Cells fetal pig cell source organ preservation cryopreservation vitrification xenotransplantation |
| title | Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research |
| title_full | Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research |
| title_fullStr | Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research |
| title_full_unstemmed | Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research |
| title_short | Exploration of Preservation Methods for Utilizing Porcine Fetal-Organ-Derived Cells in Regenerative Medicine Research |
| title_sort | exploration of preservation methods for utilizing porcine fetal organ derived cells in regenerative medicine research |
| topic | fetal pig cell source organ preservation cryopreservation vitrification xenotransplantation |
| url | https://www.mdpi.com/2073-4409/13/3/228 |
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