The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line
Adhesion is one of the main factors responsible for the probiotic properties of bacteria in the human gut. Membrane proteins affected by cellular damage are one of the key aspects determining adhesion. Fluid-bed-dried preparations containing probiotic bacteria were analyzed in terms of their stabili...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/15/3484 |
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| author | Jakub Kiepś Wojciech Juzwa Anna Olejnik Anna Sip Jolanta Tomaszewska-Gras Radosław Dembczyński |
| author_facet | Jakub Kiepś Wojciech Juzwa Anna Olejnik Anna Sip Jolanta Tomaszewska-Gras Radosław Dembczyński |
| author_sort | Jakub Kiepś |
| collection | DOAJ |
| description | Adhesion is one of the main factors responsible for the probiotic properties of bacteria in the human gut. Membrane proteins affected by cellular damage are one of the key aspects determining adhesion. Fluid-bed-dried preparations containing probiotic bacteria were analyzed in terms of their stability (temperature of glass transition) and shelf life in different conditions (modified atmosphere, refrigeration). Imaging flow cytometry was utilized to determine four subpopulations of cells based on their physiological and morphological properties. Lastly, adhesion was measured in bacteria cultured in optimal conditions and treated with heat shock. The results show that the subpopulations with no or low levels of cell membrane damage exhibit the ability to adhere to Caco-2 cells. The temperature of protein denaturation in bacteria was recorded as being between 65 °C and 70 °C. The highest glass transition temperature (Tg) value for hydroxypropyl methylcellulose (used as a coating substance) was measured at 152.6 °C. Drying and coating can be utilized as a sufficient treatment, allowing a long shelf-life (up to 12 months). It is, however, worth noting that technological processing, especially with high temperatures, may decrease the probiotic value of the preparation by damaging the bacterial cells. |
| first_indexed | 2024-03-11T00:19:23Z |
| format | Article |
| id | doaj.art-3b9fc0a8a4984e43806101f0c532c578 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T00:19:23Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-3b9fc0a8a4984e43806101f0c532c5782023-11-18T23:25:13ZengMDPI AGNutrients2072-66432023-08-011515348410.3390/nu15153484The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell LineJakub Kiepś0Wojciech Juzwa1Anna Olejnik2Anna Sip3Jolanta Tomaszewska-Gras4Radosław Dembczyński5Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60-627 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60-627 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60-627 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60-627 Poznan, PolandDepartment of Food Safety and Quality Management, Poznan University of Life Sciences, 60-624 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60-627 Poznan, PolandAdhesion is one of the main factors responsible for the probiotic properties of bacteria in the human gut. Membrane proteins affected by cellular damage are one of the key aspects determining adhesion. Fluid-bed-dried preparations containing probiotic bacteria were analyzed in terms of their stability (temperature of glass transition) and shelf life in different conditions (modified atmosphere, refrigeration). Imaging flow cytometry was utilized to determine four subpopulations of cells based on their physiological and morphological properties. Lastly, adhesion was measured in bacteria cultured in optimal conditions and treated with heat shock. The results show that the subpopulations with no or low levels of cell membrane damage exhibit the ability to adhere to Caco-2 cells. The temperature of protein denaturation in bacteria was recorded as being between 65 °C and 70 °C. The highest glass transition temperature (Tg) value for hydroxypropyl methylcellulose (used as a coating substance) was measured at 152.6 °C. Drying and coating can be utilized as a sufficient treatment, allowing a long shelf-life (up to 12 months). It is, however, worth noting that technological processing, especially with high temperatures, may decrease the probiotic value of the preparation by damaging the bacterial cells.https://www.mdpi.com/2072-6643/15/15/3484viabilityfluid bed dryinglactic acid bacteriastressquality controlimaging flow cytometry |
| spellingShingle | Jakub Kiepś Wojciech Juzwa Anna Olejnik Anna Sip Jolanta Tomaszewska-Gras Radosław Dembczyński The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line Nutrients viability fluid bed drying lactic acid bacteria stress quality control imaging flow cytometry |
| title | The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line |
| title_full | The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line |
| title_fullStr | The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line |
| title_full_unstemmed | The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line |
| title_short | The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line |
| title_sort | effects of cellular membrane damage on the long term storage and adhesion of probiotic bacteria in caco 2 cell line |
| topic | viability fluid bed drying lactic acid bacteria stress quality control imaging flow cytometry |
| url | https://www.mdpi.com/2072-6643/15/15/3484 |
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