Acetate correlates with disability and immune response in multiple sclerosis
Background Gut microbiota has been related to multiple sclerosis (MS) etiopathogenesis. Short-chain fatty acids (SCFA) are compounds derived from microbial metabolism that have a role in gut-brain axis. Objectives To analyse SCFA levels in plasma of MS patients and healthy donors (HD), and the possi...
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| Format: | Article |
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PeerJ Inc.
2020-11-01
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| Online Access: | https://peerj.com/articles/10220.pdf |
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| author | Silvia Pérez-Pérez María Inmaculada Domínguez-Mozo Aitana Alonso-Gómez Silvia Medina Noelia Villarrubia Jose Ignacio Fernández-Velasco María Ángel García-Martínez Estefanía García-Calvo Héctor Estévez Lucienne Costa-Frossard Jose C. Alvarez-Cermeño Jose L. Luque-Garcia Rafael Arroyo Luisa M. Villar Roberto Alvarez-Lafuente |
| author_facet | Silvia Pérez-Pérez María Inmaculada Domínguez-Mozo Aitana Alonso-Gómez Silvia Medina Noelia Villarrubia Jose Ignacio Fernández-Velasco María Ángel García-Martínez Estefanía García-Calvo Héctor Estévez Lucienne Costa-Frossard Jose C. Alvarez-Cermeño Jose L. Luque-Garcia Rafael Arroyo Luisa M. Villar Roberto Alvarez-Lafuente |
| author_sort | Silvia Pérez-Pérez |
| collection | DOAJ |
| description | Background Gut microbiota has been related to multiple sclerosis (MS) etiopathogenesis. Short-chain fatty acids (SCFA) are compounds derived from microbial metabolism that have a role in gut-brain axis. Objectives To analyse SCFA levels in plasma of MS patients and healthy donors (HD), and the possible link between these levels and both clinical data and immune cell populations. Methods Ninety-five MS patients and 54 HD were recruited. Patients were selected according to their score in the Expanded Disability Status Scale (EDSS) (49 EDSS ≤ 1.5, 46 EDSS ≥ 5.0). SCFA were studied in plasma samples by liquid chromatography-mass spectrometry. Peripheral blood mononuclear cells were studied by flow cytometry. Gender, age, treatments, EDSS and Multiple Sclerosis Severity Score (MSSS) were evaluated at the recruitment. Results Plasma acetate levels were higher in patients than in HD (p = 0.003). Patients with EDSS ≥ 5.0 had higher acetate levels than those with EDSS≤ 1.5 (p = 0.029), and HD (p = 2.97e–4). Acetate levels correlated with EDSS (r = 0.387; p = 1.08e–4) and MSSS (r = 0.265; p = 0.011). In untreated MS patients, acetate levels correlated inversely with CD4+ naïve T cells (r = − 0.550, p = 0.001) and directly with CD8+ IL-17+ cells (r = 0.557; p = 0.001). Conclusions Plasma acetate levels are higher in MS patients than in HD. In MS there exists a correlation between plasma acetate levels, EDSS and increased IL-17+ T cells. Future studies will elucidate the role of SCFA in the disease. |
| first_indexed | 2024-03-09T06:55:56Z |
| format | Article |
| id | doaj.art-3ba026cb7c13414f92934cd5c0344f11 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:55:56Z |
| publishDate | 2020-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-3ba026cb7c13414f92934cd5c0344f112023-12-03T10:04:00ZengPeerJ Inc.PeerJ2167-83592020-11-018e1022010.7717/peerj.10220Acetate correlates with disability and immune response in multiple sclerosisSilvia Pérez-Pérez0María Inmaculada Domínguez-Mozo1Aitana Alonso-Gómez2Silvia Medina3Noelia Villarrubia4Jose Ignacio Fernández-Velasco5María Ángel García-Martínez6Estefanía García-Calvo7Héctor Estévez8Lucienne Costa-Frossard9Jose C. Alvarez-Cermeño10Jose L. Luque-Garcia11Rafael Arroyo12Luisa M. Villar13Roberto Alvarez-Lafuente14Grupo de Investigación de Factores Ambientales en Enfermedades Degenerativas, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainGrupo de Investigación de Factores Ambientales en Enfermedades Degenerativas, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainGrupo de Investigación de Factores Ambientales en Enfermedades Degenerativas, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainServicio de Inmunología, Hospital Universitario Ramón y Cajal. Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainServicio de Inmunología, Hospital Universitario Ramón y Cajal. Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainServicio de Inmunología, Hospital Universitario Ramón y Cajal. Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainGrupo de Investigación de Factores Ambientales en Enfermedades Degenerativas, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemical Sciences, Universidad Complutense de Madrid, Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemical Sciences, Universidad Complutense de Madrid, Madrid, SpainServicio de Neurología, Hospital Universitario Ramón y Cajal / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainServicio de Neurología, Hospital Universitario Ramón y Cajal / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemical Sciences, Universidad Complutense de Madrid, Madrid, SpainDepartment of Neurology, Hospital Universitario Quironsalud Madrid / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainServicio de Inmunología, Hospital Universitario Ramón y Cajal. Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainGrupo de Investigación de Factores Ambientales en Enfermedades Degenerativas, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC) / Red Española de Esclerosis Múltiple (REEM), Madrid, SpainBackground Gut microbiota has been related to multiple sclerosis (MS) etiopathogenesis. Short-chain fatty acids (SCFA) are compounds derived from microbial metabolism that have a role in gut-brain axis. Objectives To analyse SCFA levels in plasma of MS patients and healthy donors (HD), and the possible link between these levels and both clinical data and immune cell populations. Methods Ninety-five MS patients and 54 HD were recruited. Patients were selected according to their score in the Expanded Disability Status Scale (EDSS) (49 EDSS ≤ 1.5, 46 EDSS ≥ 5.0). SCFA were studied in plasma samples by liquid chromatography-mass spectrometry. Peripheral blood mononuclear cells were studied by flow cytometry. Gender, age, treatments, EDSS and Multiple Sclerosis Severity Score (MSSS) were evaluated at the recruitment. Results Plasma acetate levels were higher in patients than in HD (p = 0.003). Patients with EDSS ≥ 5.0 had higher acetate levels than those with EDSS≤ 1.5 (p = 0.029), and HD (p = 2.97e–4). Acetate levels correlated with EDSS (r = 0.387; p = 1.08e–4) and MSSS (r = 0.265; p = 0.011). In untreated MS patients, acetate levels correlated inversely with CD4+ naïve T cells (r = − 0.550, p = 0.001) and directly with CD8+ IL-17+ cells (r = 0.557; p = 0.001). Conclusions Plasma acetate levels are higher in MS patients than in HD. In MS there exists a correlation between plasma acetate levels, EDSS and increased IL-17+ T cells. Future studies will elucidate the role of SCFA in the disease.https://peerj.com/articles/10220.pdfMultiple sclerosisShort chain fatty acidsAcetatePlasmaEDSS |
| spellingShingle | Silvia Pérez-Pérez María Inmaculada Domínguez-Mozo Aitana Alonso-Gómez Silvia Medina Noelia Villarrubia Jose Ignacio Fernández-Velasco María Ángel García-Martínez Estefanía García-Calvo Héctor Estévez Lucienne Costa-Frossard Jose C. Alvarez-Cermeño Jose L. Luque-Garcia Rafael Arroyo Luisa M. Villar Roberto Alvarez-Lafuente Acetate correlates with disability and immune response in multiple sclerosis PeerJ Multiple sclerosis Short chain fatty acids Acetate Plasma EDSS |
| title | Acetate correlates with disability and immune response in multiple sclerosis |
| title_full | Acetate correlates with disability and immune response in multiple sclerosis |
| title_fullStr | Acetate correlates with disability and immune response in multiple sclerosis |
| title_full_unstemmed | Acetate correlates with disability and immune response in multiple sclerosis |
| title_short | Acetate correlates with disability and immune response in multiple sclerosis |
| title_sort | acetate correlates with disability and immune response in multiple sclerosis |
| topic | Multiple sclerosis Short chain fatty acids Acetate Plasma EDSS |
| url | https://peerj.com/articles/10220.pdf |
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