Multimodal holographic microscopy: distinction between apoptosis and oncosis.
Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0121674 |
| _version_ | 1818590050095464448 |
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| author | Jan Balvan Aneta Krizova Jaromir Gumulec Martina Raudenska Zbysek Sladek Miroslava Sedlackova Petr Babula Marketa Sztalmachova Rene Kizek Radim Chmelik Michal Masarik |
| author_facet | Jan Balvan Aneta Krizova Jaromir Gumulec Martina Raudenska Zbysek Sladek Miroslava Sedlackova Petr Babula Marketa Sztalmachova Rene Kizek Radim Chmelik Michal Masarik |
| author_sort | Jan Balvan |
| collection | DOAJ |
| description | Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI- phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis. |
| first_indexed | 2024-12-16T09:50:22Z |
| format | Article |
| id | doaj.art-3ba07150223d42578a5d0485b0fd3c4e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:50:22Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3ba07150223d42578a5d0485b0fd3c4e2022-12-21T22:36:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012167410.1371/journal.pone.0121674Multimodal holographic microscopy: distinction between apoptosis and oncosis.Jan BalvanAneta KrizovaJaromir GumulecMartina RaudenskaZbysek SladekMiroslava SedlackovaPetr BabulaMarketa SztalmachovaRene KizekRadim ChmelikMichal MasarikIdentification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI- phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.https://doi.org/10.1371/journal.pone.0121674 |
| spellingShingle | Jan Balvan Aneta Krizova Jaromir Gumulec Martina Raudenska Zbysek Sladek Miroslava Sedlackova Petr Babula Marketa Sztalmachova Rene Kizek Radim Chmelik Michal Masarik Multimodal holographic microscopy: distinction between apoptosis and oncosis. PLoS ONE |
| title | Multimodal holographic microscopy: distinction between apoptosis and oncosis. |
| title_full | Multimodal holographic microscopy: distinction between apoptosis and oncosis. |
| title_fullStr | Multimodal holographic microscopy: distinction between apoptosis and oncosis. |
| title_full_unstemmed | Multimodal holographic microscopy: distinction between apoptosis and oncosis. |
| title_short | Multimodal holographic microscopy: distinction between apoptosis and oncosis. |
| title_sort | multimodal holographic microscopy distinction between apoptosis and oncosis |
| url | https://doi.org/10.1371/journal.pone.0121674 |
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