Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative
The antibacterial activity of <i>N</i>,<i>O</i>-acylated chitosan derivative with linoleic acid (CH_LA) was tested by disc and well diffusion, agar impregnation and microdilution methods against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/1/107 |
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| author | Agnieszka Piegat Anna Żywicka Agata Niemczyk Agata Goszczyńska |
| author_facet | Agnieszka Piegat Anna Żywicka Agata Niemczyk Agata Goszczyńska |
| author_sort | Agnieszka Piegat |
| collection | DOAJ |
| description | The antibacterial activity of <i>N</i>,<i>O</i>-acylated chitosan derivative with linoleic acid (CH_LA) was tested by disc and well diffusion, agar impregnation and microdilution methods against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Helicobacter pylori</i> strains. Hydrophobically modified chitosan (HMC) was expected to exhibit enhanced antibacterial activity and specific mucin interactions. Although diffusion tests have not indicated the antibacterial potential of chitosan (CH) or CH_LA, the results of the microdilution method demonstrated that tested polymers significantly reduced the amount of living bacteria cells in different concentrations depending on the microorganism. Additionally, CH_LA was characterized by enhanced antibacterial activity compared to CH, which may suggest a different mechanism of interaction with <i>S. aureus</i> and <i>H. pylori</i>. Furthermore, the UV-VIS analysis revealed that the amphiphilic character of derivative led to strong CH_LA–mucin interactions. The study proved the high potential of CH_LA in antibacterial applications, especially for the gastrointestinal tract. |
| first_indexed | 2024-03-10T13:41:28Z |
| format | Article |
| id | doaj.art-3ba2799d531247899abbb68daef9cf43 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T13:41:28Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-3ba2799d531247899abbb68daef9cf432023-11-21T02:58:51ZengMDPI AGPolymers2073-43602020-12-0113110710.3390/polym13010107Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan DerivativeAgnieszka Piegat0Anna Żywicka1Agata Niemczyk2Agata Goszczyńska3Department of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, 45 Piastow Ave, 70-311 Szczecin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 45 Piastow Ave, 70-311 Szczecin, PolandDepartment of Materials Technology, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology, 19 Piastow Ave, 70-310 Szczecin, PolandDepartment of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, 45 Piastow Ave, 70-311 Szczecin, PolandThe antibacterial activity of <i>N</i>,<i>O</i>-acylated chitosan derivative with linoleic acid (CH_LA) was tested by disc and well diffusion, agar impregnation and microdilution methods against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Helicobacter pylori</i> strains. Hydrophobically modified chitosan (HMC) was expected to exhibit enhanced antibacterial activity and specific mucin interactions. Although diffusion tests have not indicated the antibacterial potential of chitosan (CH) or CH_LA, the results of the microdilution method demonstrated that tested polymers significantly reduced the amount of living bacteria cells in different concentrations depending on the microorganism. Additionally, CH_LA was characterized by enhanced antibacterial activity compared to CH, which may suggest a different mechanism of interaction with <i>S. aureus</i> and <i>H. pylori</i>. Furthermore, the UV-VIS analysis revealed that the amphiphilic character of derivative led to strong CH_LA–mucin interactions. The study proved the high potential of CH_LA in antibacterial applications, especially for the gastrointestinal tract.https://www.mdpi.com/2073-4360/13/1/107hydrophobically modified chitosanmucin<i>Helicobacter pylori</i> |
| spellingShingle | Agnieszka Piegat Anna Żywicka Agata Niemczyk Agata Goszczyńska Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative Polymers hydrophobically modified chitosan mucin <i>Helicobacter pylori</i> |
| title | Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative |
| title_full | Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative |
| title_fullStr | Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative |
| title_full_unstemmed | Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative |
| title_short | Antibacterial Activity of <i>N</i>,<i>O</i>-Acylated Chitosan Derivative |
| title_sort | antibacterial activity of i n i i o i acylated chitosan derivative |
| topic | hydrophobically modified chitosan mucin <i>Helicobacter pylori</i> |
| url | https://www.mdpi.com/2073-4360/13/1/107 |
| work_keys_str_mv | AT agnieszkapiegat antibacterialactivityofiniioiacylatedchitosanderivative AT annazywicka antibacterialactivityofiniioiacylatedchitosanderivative AT agataniemczyk antibacterialactivityofiniioiacylatedchitosanderivative AT agatagoszczynska antibacterialactivityofiniioiacylatedchitosanderivative |