Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid
Linolenic acid (LA) is gaining more interest within the scientific community. This is because it has a potential medical role in reducing the risk of inflammation, carcinogenesis, atherosclerosis and diabetes and is a valuable nutraceutical for human health. The oleaginous fungus <i>Mucor circ...
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/1420-3049/27/17/5511 |
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| author | Junhuan Yang Xiuwen Wang Hassan Mohamed Shaoqi Li Chen Wu Wenyue Shi Futing Xue Sergio López-García Yuanda Song |
| author_facet | Junhuan Yang Xiuwen Wang Hassan Mohamed Shaoqi Li Chen Wu Wenyue Shi Futing Xue Sergio López-García Yuanda Song |
| author_sort | Junhuan Yang |
| collection | DOAJ |
| description | Linolenic acid (LA) is gaining more interest within the scientific community. This is because it has a potential medical role in reducing the risk of inflammation, carcinogenesis, atherosclerosis and diabetes and is a valuable nutraceutical for human health. The oleaginous fungus <i>Mucor circinelloides</i> produces a high lipid content (36%), including valuable polyunsaturated fatty acids (PUFAs). However, the critical step in which oleic acid (OA) is converted into LA is not efficient at supplying enough substrates for PUFA synthesis. Hence, we propose a method to increase LA production based on genetic engineering. The overexpression of the Δ12-desaturase gene from <i>M. circinelloides</i> and <i>Mortierella alpina</i> increased the LA content and improved the lipid accumulation (from 14.9% to 21.6% in the Δ12-desaturase gene of the <i>M. circinelloides</i> overexpressing strain (Mc-D12MC) and from 14.9% to 18.7% in the Δ12-desaturase gene of <i>M. alpina</i> overexpressing strain (Mc-D12MA)). Additionally, the up-regulated expression levels of these genes targeted the genes involved in NADPH production, implying that the elevated Δ12-desaturase gene may function as a critical regulator of NADPH and lipid synthesis in <i>M. circinelloides</i>. This study provides the first evidence to support the design of metabolic engineering related to LA and PUFA production in <i>M. circinelloides</i> for potential industrial applications. |
| first_indexed | 2024-03-10T01:30:29Z |
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| language | English |
| last_indexed | 2024-03-10T01:30:29Z |
| publishDate | 2022-08-01 |
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| series | Molecules |
| spelling | doaj.art-3ba7c981468f41bdbaebbd09e48d33fd2023-11-23T13:43:15ZengMDPI AGMolecules1420-30492022-08-012717551110.3390/molecules27175511Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic AcidJunhuan Yang0Xiuwen Wang1Hassan Mohamed2Shaoqi Li3Chen Wu4Wenyue Shi5Futing Xue6Sergio López-García7Yuanda Song8Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaDepartment of Genetics and Microbiology (Associated Unit to IQFR-CSIC), Faculty of Biology, University of Murcia, 3100 Murcia, SpainColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaLinolenic acid (LA) is gaining more interest within the scientific community. This is because it has a potential medical role in reducing the risk of inflammation, carcinogenesis, atherosclerosis and diabetes and is a valuable nutraceutical for human health. The oleaginous fungus <i>Mucor circinelloides</i> produces a high lipid content (36%), including valuable polyunsaturated fatty acids (PUFAs). However, the critical step in which oleic acid (OA) is converted into LA is not efficient at supplying enough substrates for PUFA synthesis. Hence, we propose a method to increase LA production based on genetic engineering. The overexpression of the Δ12-desaturase gene from <i>M. circinelloides</i> and <i>Mortierella alpina</i> increased the LA content and improved the lipid accumulation (from 14.9% to 21.6% in the Δ12-desaturase gene of the <i>M. circinelloides</i> overexpressing strain (Mc-D12MC) and from 14.9% to 18.7% in the Δ12-desaturase gene of <i>M. alpina</i> overexpressing strain (Mc-D12MA)). Additionally, the up-regulated expression levels of these genes targeted the genes involved in NADPH production, implying that the elevated Δ12-desaturase gene may function as a critical regulator of NADPH and lipid synthesis in <i>M. circinelloides</i>. This study provides the first evidence to support the design of metabolic engineering related to LA and PUFA production in <i>M. circinelloides</i> for potential industrial applications.https://www.mdpi.com/1420-3049/27/17/5511homologous expressionheterologous expression<i>Mucor circinelloides</i>delta-12 desaturaselipid accumulationlinolenic acid production |
| spellingShingle | Junhuan Yang Xiuwen Wang Hassan Mohamed Shaoqi Li Chen Wu Wenyue Shi Futing Xue Sergio López-García Yuanda Song Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid Molecules homologous expression heterologous expression <i>Mucor circinelloides</i> delta-12 desaturase lipid accumulation linolenic acid production |
| title | Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid |
| title_full | Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid |
| title_fullStr | Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid |
| title_full_unstemmed | Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid |
| title_short | Homologous and Heterologous Expression of Delta(12)-Desaturase in <i>Mucor circinelloides</i> Enhanced the Production of Linolenic Acid |
| title_sort | homologous and heterologous expression of delta 12 desaturase in i mucor circinelloides i enhanced the production of linolenic acid |
| topic | homologous expression heterologous expression <i>Mucor circinelloides</i> delta-12 desaturase lipid accumulation linolenic acid production |
| url | https://www.mdpi.com/1420-3049/27/17/5511 |
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