Insertion sequence transposition inactivates CRISPR-Cas immunity
Abstract CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified multiple natural transpositions of insertion sequences (ISs) into cas genes, thus inactivating CRISPR-Cas defenses....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-39964-7 |
| _version_ | 1797774075573043200 |
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| author | Yong Sheng Hengyu Wang Yixin Ou Yingying Wu Wei Ding Meifeng Tao Shuangjun Lin Zixin Deng Linquan Bai Qianjin Kang |
| author_facet | Yong Sheng Hengyu Wang Yixin Ou Yingying Wu Wei Ding Meifeng Tao Shuangjun Lin Zixin Deng Linquan Bai Qianjin Kang |
| author_sort | Yong Sheng |
| collection | DOAJ |
| description | Abstract CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified multiple natural transpositions of insertion sequences (ISs) into cas genes, thus inactivating CRISPR-Cas defenses. We then generated an IS-trapping system, using Escherichia coli strains with various ISs and an inducible cas nuclease, to monitor IS insertions into cas genes following the induction of double-strand DNA breakage as a physiological host stress. We identified multiple events mediated by different ISs, especially IS1 and IS10, displaying substantial relaxed target specificity. IS transposition into cas was maintained in the presence of DNA repair machinery, and transposition into other host defense systems was also detected. Our findings highlight the potential of ISs to counter CRISPR activity, thus increasing bacterial susceptibility to foreign DNA invasion. |
| first_indexed | 2024-03-12T22:15:45Z |
| format | Article |
| id | doaj.art-3ba99588949d43d9b6f109ea27b45e01 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-12T22:15:45Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-3ba99588949d43d9b6f109ea27b45e012023-07-23T11:18:56ZengNature PortfolioNature Communications2041-17232023-07-0114111910.1038/s41467-023-39964-7Insertion sequence transposition inactivates CRISPR-Cas immunityYong Sheng0Hengyu Wang1Yixin Ou2Yingying Wu3Wei Ding4Meifeng Tao5Shuangjun Lin6Zixin Deng7Linquan Bai8Qianjin Kang9State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityAbstract CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified multiple natural transpositions of insertion sequences (ISs) into cas genes, thus inactivating CRISPR-Cas defenses. We then generated an IS-trapping system, using Escherichia coli strains with various ISs and an inducible cas nuclease, to monitor IS insertions into cas genes following the induction of double-strand DNA breakage as a physiological host stress. We identified multiple events mediated by different ISs, especially IS1 and IS10, displaying substantial relaxed target specificity. IS transposition into cas was maintained in the presence of DNA repair machinery, and transposition into other host defense systems was also detected. Our findings highlight the potential of ISs to counter CRISPR activity, thus increasing bacterial susceptibility to foreign DNA invasion.https://doi.org/10.1038/s41467-023-39964-7 |
| spellingShingle | Yong Sheng Hengyu Wang Yixin Ou Yingying Wu Wei Ding Meifeng Tao Shuangjun Lin Zixin Deng Linquan Bai Qianjin Kang Insertion sequence transposition inactivates CRISPR-Cas immunity Nature Communications |
| title | Insertion sequence transposition inactivates CRISPR-Cas immunity |
| title_full | Insertion sequence transposition inactivates CRISPR-Cas immunity |
| title_fullStr | Insertion sequence transposition inactivates CRISPR-Cas immunity |
| title_full_unstemmed | Insertion sequence transposition inactivates CRISPR-Cas immunity |
| title_short | Insertion sequence transposition inactivates CRISPR-Cas immunity |
| title_sort | insertion sequence transposition inactivates crispr cas immunity |
| url | https://doi.org/10.1038/s41467-023-39964-7 |
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