Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins
Three different macroporous resins (XAD7HP, DAX-8, and XAD4) were evaluated for their adsorption and desorption properties in preparing flavonoid-enriched oil palm (<i>Elaeis guineensis</i> Jacq.) leaf extract. The influences of initial concentration, solution pH, contact time, and desor...
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MDPI AG
2020-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/4/778 |
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| author | Mohamad Shazeli Che Zain Soo Yee Lee Chian Ying Teo Khozirah Shaari |
| author_facet | Mohamad Shazeli Che Zain Soo Yee Lee Chian Ying Teo Khozirah Shaari |
| author_sort | Mohamad Shazeli Che Zain |
| collection | DOAJ |
| description | Three different macroporous resins (XAD7HP, DAX-8, and XAD4) were evaluated for their adsorption and desorption properties in preparing flavonoid-enriched oil palm (<i>Elaeis guineensis</i> Jacq.) leaf extract. The influences of initial concentration, solution pH, contact time, and desorption solvent (ethanol) concentration were determined by static sorption/desorption methods. The optimal condition for adsorption of flavonoids was achieved when the solution of the extract was adjusted to pH 7, reaching equilibrium after 1440 min at 298 K. The adsorption process was well described by a pseudo-second-order kinetics model, while the adsorption isotherm data fitted well with a Freundlich model. The adsorption by each resin was via an exothermic and physical adsorption process. Based on the static experiment results, XAD7HP was found to be the most appropriate adsorbent, while 80% ethanol was the best solvent for desorbent. Further evaluation of its dynamic adsorption and desorption characteristics on a packed glass column showed that XAD7HP could enrich the OPL total flavonoid content by a 3.57-fold increment. Moreover, UHPLC−UV/PDA and UHPLC−MS/MS analysis revealed that apigenin and luteolin derivatives were selectively adsorbed by XAD7HP.<i> </i>Additionally, both the crude OPL extract and the flavonoid-enriched fraction have good DPPH and NO free radical scavenging activities. Multiple interactions between the flavonoids and cross-linked polymeric XAD7HP resin through van der Waals forces and hydrogen bonding described the sorption processes. Therefore, by utilizing this method, the flavonoid-enriched fraction from crude OPL extract could be used as a potential bioactive ingredient in nutraceutical and pharmaceutical applications at minimum cost with optimum efficiency. |
| first_indexed | 2024-12-10T17:09:36Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T17:09:36Z |
| publishDate | 2020-02-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3babdec3b3554da2836d112ac2df0e5f2022-12-22T01:40:22ZengMDPI AGMolecules1420-30492020-02-0125477810.3390/molecules25040778molecules25040778Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption ResinsMohamad Shazeli Che Zain0Soo Yee Lee1Chian Ying Teo2Khozirah Shaari3Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, 57000 Kuala Lumpur, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaThree different macroporous resins (XAD7HP, DAX-8, and XAD4) were evaluated for their adsorption and desorption properties in preparing flavonoid-enriched oil palm (<i>Elaeis guineensis</i> Jacq.) leaf extract. The influences of initial concentration, solution pH, contact time, and desorption solvent (ethanol) concentration were determined by static sorption/desorption methods. The optimal condition for adsorption of flavonoids was achieved when the solution of the extract was adjusted to pH 7, reaching equilibrium after 1440 min at 298 K. The adsorption process was well described by a pseudo-second-order kinetics model, while the adsorption isotherm data fitted well with a Freundlich model. The adsorption by each resin was via an exothermic and physical adsorption process. Based on the static experiment results, XAD7HP was found to be the most appropriate adsorbent, while 80% ethanol was the best solvent for desorbent. Further evaluation of its dynamic adsorption and desorption characteristics on a packed glass column showed that XAD7HP could enrich the OPL total flavonoid content by a 3.57-fold increment. Moreover, UHPLC−UV/PDA and UHPLC−MS/MS analysis revealed that apigenin and luteolin derivatives were selectively adsorbed by XAD7HP.<i> </i>Additionally, both the crude OPL extract and the flavonoid-enriched fraction have good DPPH and NO free radical scavenging activities. Multiple interactions between the flavonoids and cross-linked polymeric XAD7HP resin through van der Waals forces and hydrogen bonding described the sorption processes. Therefore, by utilizing this method, the flavonoid-enriched fraction from crude OPL extract could be used as a potential bioactive ingredient in nutraceutical and pharmaceutical applications at minimum cost with optimum efficiency.https://www.mdpi.com/1420-3049/25/4/778oil palm (<i>elaeis guineensis</i> jacq.) leaftotal flavonoidsmacroporous resinsadsorptiondesorptionantioxidant activity |
| spellingShingle | Mohamad Shazeli Che Zain Soo Yee Lee Chian Ying Teo Khozirah Shaari Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins Molecules oil palm (<i>elaeis guineensis</i> jacq.) leaf total flavonoids macroporous resins adsorption desorption antioxidant activity |
| title | Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins |
| title_full | Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins |
| title_fullStr | Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins |
| title_full_unstemmed | Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins |
| title_short | Adsorption and Desorption Properties of Total Flavonoids from Oil Palm (<i>Elaeis guineensis</i> Jacq.) Mature Leaf on Macroporous Adsorption Resins |
| title_sort | adsorption and desorption properties of total flavonoids from oil palm i elaeis guineensis i jacq mature leaf on macroporous adsorption resins |
| topic | oil palm (<i>elaeis guineensis</i> jacq.) leaf total flavonoids macroporous resins adsorption desorption antioxidant activity |
| url | https://www.mdpi.com/1420-3049/25/4/778 |
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