Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)
Neurocysticercosis (NCC), one of the most important neuroparasitic diseases in humans, is caused by Cysticercus cellulosae, the metacestode stage of digenetic zoonotic cestode Taenia solium. The present study aims at the detection of anti-cysticercus antibodies in the sera of epileptic patients (n=2...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2023-12-01
|
| Series: | Helminthologia |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/helm-2023-0023 |
| _version_ | 1797368785721622528 |
|---|---|
| author | Satyaprakash K. Khan W. A. Zade N. N. Chaudhari S. P. Shinde S. V. Kurkure N. V. Shembalkar P. K. |
| author_facet | Satyaprakash K. Khan W. A. Zade N. N. Chaudhari S. P. Shinde S. V. Kurkure N. V. Shembalkar P. K. |
| author_sort | Satyaprakash K. |
| collection | DOAJ |
| description | Neurocysticercosis (NCC), one of the most important neuroparasitic diseases in humans, is caused by Cysticercus cellulosae, the metacestode stage of digenetic zoonotic cestode Taenia solium. The present study aims at the detection of anti-cysticercus antibodies in the sera of epileptic patients (n=26) visiting a tertiary care hospital in Nagpur, Maharashtra state, India, by an in-house developed indirect IgG-ELISA and enzyme-linked immunoelectro transfer blot (EITB) assay using different antigens (namely, Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA), Excretory-Secretory Antigen (ESA) and Membrane-Body Antigen (MBA)) prepared from T. solium metacestodes to find out the status of NCC. An attempt has also been made for molecular detection of NCC from blood samples of those patients by Polymerase Chain Reaction (PCR) assay targeted at large subunit rRNA gene of T. solium. The IgG ELISA level of anti-cysticercus antibodies against WCA, CFA, SA, ESA and MBA antigens were as follows: 19.23 %, 23.07 %, 38.46 %, 30.76 % and 15.38 %. The seroreactivity to CFA, SA and ESA was found in equal proportions in patients with ring-enhancing lesions. In the EITB assay, the lower and medium molecular weight protein bands of SA and ESA were immunodominant compared to the higher WCA and CFA peptides. PCR positivity could be observed in 34.6 % (9/26) of the patients under study. It is the first report of detecting NCC among epileptic patients of the Nagpur region of Maharashtra state in India using serological and molecular tools. |
| first_indexed | 2024-03-08T17:37:45Z |
| format | Article |
| id | doaj.art-3bb129dc4370451a8eeea8552a20fce2 |
| institution | Directory Open Access Journal |
| issn | 1336-9083 |
| language | English |
| last_indexed | 2024-03-08T17:37:45Z |
| publishDate | 2023-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-3bb129dc4370451a8eeea8552a20fce22024-01-02T11:35:22ZengSciendoHelminthologia1336-90832023-12-0160320822010.2478/helm-2023-0023Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)Satyaprakash K.0Khan W. A.1Zade N. N.2Chaudhari S. P.3Shinde S. V.4Kurkure N. V.5Shembalkar P. K.6Department of Veterinary Public Health & Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India-440006Department of Veterinary Public Health & Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India-440006Department of Veterinary Public Health & Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India-440006Department of Veterinary Public Health & Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India-440006Department of Veterinary Public Health & Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India-440006Department of Veterinary Pathology, Nagpur Veterinary College, Nagpur, Maharashtra, India-440006Get Well Hospital and Research Institute, Nagpur, Maharashtra, India-440012Neurocysticercosis (NCC), one of the most important neuroparasitic diseases in humans, is caused by Cysticercus cellulosae, the metacestode stage of digenetic zoonotic cestode Taenia solium. The present study aims at the detection of anti-cysticercus antibodies in the sera of epileptic patients (n=26) visiting a tertiary care hospital in Nagpur, Maharashtra state, India, by an in-house developed indirect IgG-ELISA and enzyme-linked immunoelectro transfer blot (EITB) assay using different antigens (namely, Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA), Excretory-Secretory Antigen (ESA) and Membrane-Body Antigen (MBA)) prepared from T. solium metacestodes to find out the status of NCC. An attempt has also been made for molecular detection of NCC from blood samples of those patients by Polymerase Chain Reaction (PCR) assay targeted at large subunit rRNA gene of T. solium. The IgG ELISA level of anti-cysticercus antibodies against WCA, CFA, SA, ESA and MBA antigens were as follows: 19.23 %, 23.07 %, 38.46 %, 30.76 % and 15.38 %. The seroreactivity to CFA, SA and ESA was found in equal proportions in patients with ring-enhancing lesions. In the EITB assay, the lower and medium molecular weight protein bands of SA and ESA were immunodominant compared to the higher WCA and CFA peptides. PCR positivity could be observed in 34.6 % (9/26) of the patients under study. It is the first report of detecting NCC among epileptic patients of the Nagpur region of Maharashtra state in India using serological and molecular tools.https://doi.org/10.2478/helm-2023-0023neurocysticercosistaenia soliumantigenselisaeitbpcr |
| spellingShingle | Satyaprakash K. Khan W. A. Zade N. N. Chaudhari S. P. Shinde S. V. Kurkure N. V. Shembalkar P. K. Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) Helminthologia neurocysticercosis taenia solium antigens elisa eitb pcr |
| title | Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) |
| title_full | Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) |
| title_fullStr | Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) |
| title_full_unstemmed | Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) |
| title_short | Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) |
| title_sort | serological and molecular detection of neurocysticercosis among epileptic patients in nagpur maharashtra state india |
| topic | neurocysticercosis taenia solium antigens elisa eitb pcr |
| url | https://doi.org/10.2478/helm-2023-0023 |
| work_keys_str_mv | AT satyaprakashk serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia AT khanwa serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia AT zadenn serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia AT chaudharisp serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia AT shindesv serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia AT kurkurenv serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia AT shembalkarpk serologicalandmoleculardetectionofneurocysticercosisamongepilepticpatientsinnagpurmaharashtrastateindia |