Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027
<p>Abstract</p> <p>Background</p> <p>C-1027, produced by <it>Streptomyces globisporus </it>C-1027, is one of the most potent antitumoral agents. The biosynthetic gene cluster of C-1027, previously cloned and sequenced, contains at least three putative regula...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-01-01
|
| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/14 |
| _version_ | 1811313532506996736 |
|---|---|
| author | Jiang Wei Bao Yi Cui Zhihui Wang Songmei Zhang Yanjuan Hu Yunfeng Wang Lifei Hong Bin |
| author_facet | Jiang Wei Bao Yi Cui Zhihui Wang Songmei Zhang Yanjuan Hu Yunfeng Wang Lifei Hong Bin |
| author_sort | Jiang Wei |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>C-1027, produced by <it>Streptomyces globisporus </it>C-1027, is one of the most potent antitumoral agents. The biosynthetic gene cluster of C-1027, previously cloned and sequenced, contains at least three putative regulatory genes, i.e. <it>sgcR1</it>, <it>sgcR2 </it>and <it>sgcR3</it>. The predicted gene products of these genes share sequence similarities to StrR, regulators of AraC/XylS family and TylR. The purpose of this study was to investigate the role of <it>sgcR3 </it>in C-1027 biosynthesis.</p> <p>Results</p> <p>Overexpression of <it>sgcR3 </it>in <it>S. globisporus </it>C-1027 resulted in a 30–40% increase in C-1027 production. Consistent with this, disruption of <it>sgcR3 </it>abolished C-1027 production. Complementation of the <it>sgcR3</it>-disrupted strain R3KO with intact <it>sgcR3 </it>gene could restore C-1027 production. The results from real time RT-PCR analysis in R3KO mutant and wild type strain indicated that not only transcripts of biosynthetic structural genes such as <it>sgcA1 </it>and <it>sgcC4</it>, but also putative regulatory genes, <it>sgcR1 </it>and <it>sgcR2</it>, were significantly decreased in R3KO mutant. The cross-complementation studies showed that <it>sgcR1R2 </it>could functionally complement <it>sgcR3 </it>disruption <it>in trans</it>. Purified N-terminal His<sub>10</sub>-tagged SgcR3 showed specific DNA-binding activity to the promoter region of <it>sgcR1R2</it>.</p> <p>Conclusion</p> <p>The role of SgcR3 has been proved to be a positive regulator of C-1027 biosynthesis in <it>S. globisporus </it>C-1027. SgcR3 occupies a higher level than SgcR1 and SgcR2 in the regulatory hierarchy that controls C-1027 production and activates the transcription of <it>sgcR1 </it>and <it>sgcR2 </it>by binding directly to the promoter region of <it>sgcR1R2</it>.</p> |
| first_indexed | 2024-04-13T10:55:29Z |
| format | Article |
| id | doaj.art-3bb74199d3c74a04bd0f8015c9dbc814 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T10:55:29Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-3bb74199d3c74a04bd0f8015c9dbc8142022-12-22T02:49:32ZengBMCBMC Microbiology1471-21802009-01-01911410.1186/1471-2180-9-14Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027Jiang WeiBao YiCui ZhihuiWang SongmeiZhang YanjuanHu YunfengWang LifeiHong Bin<p>Abstract</p> <p>Background</p> <p>C-1027, produced by <it>Streptomyces globisporus </it>C-1027, is one of the most potent antitumoral agents. The biosynthetic gene cluster of C-1027, previously cloned and sequenced, contains at least three putative regulatory genes, i.e. <it>sgcR1</it>, <it>sgcR2 </it>and <it>sgcR3</it>. The predicted gene products of these genes share sequence similarities to StrR, regulators of AraC/XylS family and TylR. The purpose of this study was to investigate the role of <it>sgcR3 </it>in C-1027 biosynthesis.</p> <p>Results</p> <p>Overexpression of <it>sgcR3 </it>in <it>S. globisporus </it>C-1027 resulted in a 30–40% increase in C-1027 production. Consistent with this, disruption of <it>sgcR3 </it>abolished C-1027 production. Complementation of the <it>sgcR3</it>-disrupted strain R3KO with intact <it>sgcR3 </it>gene could restore C-1027 production. The results from real time RT-PCR analysis in R3KO mutant and wild type strain indicated that not only transcripts of biosynthetic structural genes such as <it>sgcA1 </it>and <it>sgcC4</it>, but also putative regulatory genes, <it>sgcR1 </it>and <it>sgcR2</it>, were significantly decreased in R3KO mutant. The cross-complementation studies showed that <it>sgcR1R2 </it>could functionally complement <it>sgcR3 </it>disruption <it>in trans</it>. Purified N-terminal His<sub>10</sub>-tagged SgcR3 showed specific DNA-binding activity to the promoter region of <it>sgcR1R2</it>.</p> <p>Conclusion</p> <p>The role of SgcR3 has been proved to be a positive regulator of C-1027 biosynthesis in <it>S. globisporus </it>C-1027. SgcR3 occupies a higher level than SgcR1 and SgcR2 in the regulatory hierarchy that controls C-1027 production and activates the transcription of <it>sgcR1 </it>and <it>sgcR2 </it>by binding directly to the promoter region of <it>sgcR1R2</it>.</p>http://www.biomedcentral.com/1471-2180/9/14 |
| spellingShingle | Jiang Wei Bao Yi Cui Zhihui Wang Songmei Zhang Yanjuan Hu Yunfeng Wang Lifei Hong Bin Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027 BMC Microbiology |
| title | Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027 |
| title_full | Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027 |
| title_fullStr | Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027 |
| title_full_unstemmed | Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027 |
| title_short | Role of <it>sgcR3 </it>in positive regulation of enediyne antibiotic C-1027 production of <it>Streptomyces globisporus </it>C-1027 |
| title_sort | role of it sgcr3 it in positive regulation of enediyne antibiotic c 1027 production of it streptomyces globisporus it c 1027 |
| url | http://www.biomedcentral.com/1471-2180/9/14 |
| work_keys_str_mv | AT jiangwei roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT baoyi roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT cuizhihui roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT wangsongmei roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT zhangyanjuan roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT huyunfeng roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT wanglifei roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 AT hongbin roleofitsgcr3itinpositiveregulationofenediyneantibioticc1027productionofitstreptomycesglobisporusitc1027 |