Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China
Labriocimbex sinicus Yan & Wei gen. et sp. nov. of Cimbicidae is described. The new genus is similar to Praia Andre and Trichiosoma Leach. A key to extant Holarctic genera of Cimbicinae is provided. To identify the phylogenetic placement of Cimbicidae, the mitochondrial genome of L. sinicus was...
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PeerJ Inc.
2019-10-01
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| author | Yuchen Yan Gengyun Niu Yaoyao Zhang Qianying Ren Shiyu Du Bocheng Lan Meicai Wei |
| author_facet | Yuchen Yan Gengyun Niu Yaoyao Zhang Qianying Ren Shiyu Du Bocheng Lan Meicai Wei |
| author_sort | Yuchen Yan |
| collection | DOAJ |
| description | Labriocimbex sinicus Yan & Wei gen. et sp. nov. of Cimbicidae is described. The new genus is similar to Praia Andre and Trichiosoma Leach. A key to extant Holarctic genera of Cimbicinae is provided. To identify the phylogenetic placement of Cimbicidae, the mitochondrial genome of L. sinicus was annotated and characterized using high-throughput sequencing data. The complete mitochondrial genome of L. sinicus was obtained with a length of 15,405 bp (GenBank: MH136623; SRA: SRR8270383) and a typical set of 37 genes (22 tRNAs, 13 PCGs, and two rRNAs). The results demonstrated that all PCGs were initiated by ATN codon, and ended with TAA or T stop codons. The study reveals that all tRNA genes have a typical clover-leaf secondary structure, except for trnS1. Remarkably, the secondary structures of the rrnS and rrnL of L. sinicus were much different from those of Corynis lateralis. Phylogenetic analyses verified the monophyly and positions of the three Cimbicidae species within the superfamily Tenthredinoidea and demonstrated a relationship as (Tenthredinidae + Cimbicidae) + (Argidae + Pergidae) with strong nodal supports. Furthermore, we found that the generic relationships of Cimbicidae revealed by the phylogenetic analyses based on COI genes agree quite closely with the systematic arrangement of the genera based on the morphological characters. Phylogenetic tree based on two methods shows that L. sinicus is the sister group of Praia with high support values. We suggest that Labriocimbex belongs to the tribe Trichiosomini of Cimbicinae based on adult morphology and molecular data. Besides, we suggest to promote the subgenus Asitrichiosoma to be a valid genus. |
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| spelling | doaj.art-3bb7b509015a471fa0c88c9e6e2193642023-12-03T11:07:08ZengPeerJ Inc.PeerJ2167-83592019-10-017e785310.7717/peerj.7853Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from ChinaYuchen Yan0Gengyun Niu1Yaoyao Zhang2Qianying Ren3Shiyu Du4Bocheng Lan5Meicai Wei6Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees; Lab of Insect Systematics and Evolutionary Biology, Central South University of Forestry and Technology, Changsha, ChinaJiangxi Normal University, Nanchang, Jiangxi, ChinaCollege of Life Sciences, Nankai University, Tianjin, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees; Lab of Insect Systematics and Evolutionary Biology, Central South University of Forestry and Technology, Changsha, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees; Lab of Insect Systematics and Evolutionary Biology, Central South University of Forestry and Technology, Changsha, ChinaKey Laboratory of Cultivation and Protection for Non-Wood Forest Trees; Lab of Insect Systematics and Evolutionary Biology, Central South University of Forestry and Technology, Changsha, ChinaJiangxi Normal University, Nanchang, Jiangxi, ChinaLabriocimbex sinicus Yan & Wei gen. et sp. nov. of Cimbicidae is described. The new genus is similar to Praia Andre and Trichiosoma Leach. A key to extant Holarctic genera of Cimbicinae is provided. To identify the phylogenetic placement of Cimbicidae, the mitochondrial genome of L. sinicus was annotated and characterized using high-throughput sequencing data. The complete mitochondrial genome of L. sinicus was obtained with a length of 15,405 bp (GenBank: MH136623; SRA: SRR8270383) and a typical set of 37 genes (22 tRNAs, 13 PCGs, and two rRNAs). The results demonstrated that all PCGs were initiated by ATN codon, and ended with TAA or T stop codons. The study reveals that all tRNA genes have a typical clover-leaf secondary structure, except for trnS1. Remarkably, the secondary structures of the rrnS and rrnL of L. sinicus were much different from those of Corynis lateralis. Phylogenetic analyses verified the monophyly and positions of the three Cimbicidae species within the superfamily Tenthredinoidea and demonstrated a relationship as (Tenthredinidae + Cimbicidae) + (Argidae + Pergidae) with strong nodal supports. Furthermore, we found that the generic relationships of Cimbicidae revealed by the phylogenetic analyses based on COI genes agree quite closely with the systematic arrangement of the genera based on the morphological characters. Phylogenetic tree based on two methods shows that L. sinicus is the sister group of Praia with high support values. We suggest that Labriocimbex belongs to the tribe Trichiosomini of Cimbicinae based on adult morphology and molecular data. Besides, we suggest to promote the subgenus Asitrichiosoma to be a valid genus.https://peerj.com/articles/7853.pdfTaxonomyMitochondrial genomeNext generation sequencingPhylogenyHymenoptera |
| spellingShingle | Yuchen Yan Gengyun Niu Yaoyao Zhang Qianying Ren Shiyu Du Bocheng Lan Meicai Wei Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China PeerJ Taxonomy Mitochondrial genome Next generation sequencing Phylogeny Hymenoptera |
| title | Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China |
| title_full | Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China |
| title_fullStr | Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China |
| title_full_unstemmed | Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China |
| title_short | Complete mitochondrial genome sequence of Labriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China |
| title_sort | complete mitochondrial genome sequence of labriocimbex sinicus a new genus and new species of cimbicidae hymenoptera from china |
| topic | Taxonomy Mitochondrial genome Next generation sequencing Phylogeny Hymenoptera |
| url | https://peerj.com/articles/7853.pdf |
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