Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer
(1) Background: Biophysical techniques applied to serum samples characterization could promote the development of new diagnostic tools. Fluorescence spectroscopy has been previously applied to biological samples from cancer patients and differences from healthy individuals were observed. Dendronized...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6501 |
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| author | Violeta Morcuende-Ventura Sonia Hermoso-Durán Natalia Abian-Franco Roberto Pazo-Cid Jorge L. Ojeda Sonia Vega Oscar Sanchez-Gracia Adrian Velazquez-Campoy Teresa Sierra Olga Abian |
| author_facet | Violeta Morcuende-Ventura Sonia Hermoso-Durán Natalia Abian-Franco Roberto Pazo-Cid Jorge L. Ojeda Sonia Vega Oscar Sanchez-Gracia Adrian Velazquez-Campoy Teresa Sierra Olga Abian |
| author_sort | Violeta Morcuende-Ventura |
| collection | DOAJ |
| description | (1) Background: Biophysical techniques applied to serum samples characterization could promote the development of new diagnostic tools. Fluorescence spectroscopy has been previously applied to biological samples from cancer patients and differences from healthy individuals were observed. Dendronized hyperbranched polymers (DHP) based on bis(hydroxymethyl)propionic acid (bis-MPA) were developed in our group and their potential biomedical applications explored. (2) Methods: A total of 94 serum samples from diagnosed cancer patients and healthy individuals were studied (20 pancreatic ductal adenocarcinoma, 25 blood donor, 24 ovarian cancer, and 25 benign ovarian cyst samples). (3) Results: Fluorescence spectra of serum samples (fluorescence liquid biopsy, FLB) in the presence and the absence of DHP-bMPA were recorded and two parameters from the signal curves obtained. A secondary parameter, the fluorescence spectrum score (FS<sub>score</sub>), was calculated, and the diagnostic model assessed. For pancreatic ductal adenocarcinoma (PDAC) and ovarian cancer, the classification performance was improved when including DHP-bMPA, achieving high values of statistical sensitivity and specificity (over 85% for both pathologies). (4) Conclusions: We have applied FLB as a quick, simple, and minimally invasive promising technique in cancer diagnosis. The classification performance of the diagnostic method was further improved by using DHP-bMPA, which interacted differentially with serum samples from healthy and diseased subjects. These preliminary results set the basis for a larger study and move FLB closer to its clinical application, providing useful information for the oncologist during patient diagnosis. |
| first_indexed | 2024-03-10T10:19:20Z |
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| language | English |
| last_indexed | 2024-03-10T10:19:20Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3bb9813dfe22457cae1da207926137592023-11-22T00:31:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212650110.3390/ijms22126501Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched PolymerVioleta Morcuende-Ventura0Sonia Hermoso-Durán1Natalia Abian-Franco2Roberto Pazo-Cid3Jorge L. Ojeda4Sonia Vega5Oscar Sanchez-Gracia6Adrian Velazquez-Campoy7Teresa Sierra8Olga Abian9Instituto de Nanociencia y Materiales de Aragón (INMA), Química Orgánica, Facultad de Ciencias, CSIC-Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, SpainJoint Units IQFR-CSIC-BIFI and GBsC-CSIC-BIFI, Institute of Biocomputation and Physics of Complex Systems (BIFI), Universidad de Zaragoza, 50018 Zaragoza, SpainHospital Reina Sofía, Carr. Tarazona, Km. 4, 31500 Tudela, SpainHospital Universitario Miguel Servet (HUMS), Paseo Isabel la Católica, 1-3, 50009 Zaragoza, SpainDepartment of Statistical Methods, Universidad de Zaragoza, 50009 Zaragoza, SpainJoint Units IQFR-CSIC-BIFI and GBsC-CSIC-BIFI, Institute of Biocomputation and Physics of Complex Systems (BIFI), Universidad de Zaragoza, 50018 Zaragoza, SpainSOTER BioAnalytics, Enrique Val, 50011 Zaragoza, SpainJoint Units IQFR-CSIC-BIFI and GBsC-CSIC-BIFI, Institute of Biocomputation and Physics of Complex Systems (BIFI), Universidad de Zaragoza, 50018 Zaragoza, SpainInstituto de Nanociencia y Materiales de Aragón (INMA), Química Orgánica, Facultad de Ciencias, CSIC-Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, SpainJoint Units IQFR-CSIC-BIFI and GBsC-CSIC-BIFI, Institute of Biocomputation and Physics of Complex Systems (BIFI), Universidad de Zaragoza, 50018 Zaragoza, Spain(1) Background: Biophysical techniques applied to serum samples characterization could promote the development of new diagnostic tools. Fluorescence spectroscopy has been previously applied to biological samples from cancer patients and differences from healthy individuals were observed. Dendronized hyperbranched polymers (DHP) based on bis(hydroxymethyl)propionic acid (bis-MPA) were developed in our group and their potential biomedical applications explored. (2) Methods: A total of 94 serum samples from diagnosed cancer patients and healthy individuals were studied (20 pancreatic ductal adenocarcinoma, 25 blood donor, 24 ovarian cancer, and 25 benign ovarian cyst samples). (3) Results: Fluorescence spectra of serum samples (fluorescence liquid biopsy, FLB) in the presence and the absence of DHP-bMPA were recorded and two parameters from the signal curves obtained. A secondary parameter, the fluorescence spectrum score (FS<sub>score</sub>), was calculated, and the diagnostic model assessed. For pancreatic ductal adenocarcinoma (PDAC) and ovarian cancer, the classification performance was improved when including DHP-bMPA, achieving high values of statistical sensitivity and specificity (over 85% for both pathologies). (4) Conclusions: We have applied FLB as a quick, simple, and minimally invasive promising technique in cancer diagnosis. The classification performance of the diagnostic method was further improved by using DHP-bMPA, which interacted differentially with serum samples from healthy and diseased subjects. These preliminary results set the basis for a larger study and move FLB closer to its clinical application, providing useful information for the oncologist during patient diagnosis.https://www.mdpi.com/1422-0067/22/12/6501pancreatic ductal adenocarcinoma <b>(</b>PDAC)ovarian cancerfluorescence spectroscopydendrimersliquid biopsydiagnosis |
| spellingShingle | Violeta Morcuende-Ventura Sonia Hermoso-Durán Natalia Abian-Franco Roberto Pazo-Cid Jorge L. Ojeda Sonia Vega Oscar Sanchez-Gracia Adrian Velazquez-Campoy Teresa Sierra Olga Abian Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer International Journal of Molecular Sciences pancreatic ductal adenocarcinoma <b>(</b>PDAC) ovarian cancer fluorescence spectroscopy dendrimers liquid biopsy diagnosis |
| title | Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer |
| title_full | Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer |
| title_fullStr | Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer |
| title_full_unstemmed | Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer |
| title_short | Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer |
| title_sort | fluorescence liquid biopsy for cancer detection is improved by using cationic dendronized hyperbranched polymer |
| topic | pancreatic ductal adenocarcinoma <b>(</b>PDAC) ovarian cancer fluorescence spectroscopy dendrimers liquid biopsy diagnosis |
| url | https://www.mdpi.com/1422-0067/22/12/6501 |
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