Alterations of Lipid Profile in Livers with Impaired Lipophagy
Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation in the liver. Various mechanisms such as an increased uptake in fatty acids or de novo synthesis contribute to the development of steatosis and progression to more severe stages. Furthermore, it has been shown...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11863 |
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| author | Wenke Jonas Kristin Schwerbel Lisa Zellner Markus Jähnert Pascal Gottmann Annette Schürmann |
| author_facet | Wenke Jonas Kristin Schwerbel Lisa Zellner Markus Jähnert Pascal Gottmann Annette Schürmann |
| author_sort | Wenke Jonas |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation in the liver. Various mechanisms such as an increased uptake in fatty acids or de novo synthesis contribute to the development of steatosis and progression to more severe stages. Furthermore, it has been shown that impaired lipophagy, the degradation of lipids by autophagic processes, contributes to NAFLD. Through an unbiased lipidome analysis of mouse livers in a genetic model of impaired lipophagy, we aimed to determine the resulting alterations in the lipidome. Observed changes overlap with those of the human disease. Overall, the entire lipid content and in particular the triacylglycerol concentration increased under conditions of impaired lipophagy. In addition, we detected a reduction in long-chain polyunsaturated fatty acids (PUFAs) and an increased ratio of n-6 PUFAs to n-3 PUFAs, which was due to the depletion of n-3 PUFAs. Although the abundance of major phospholipid classes was reduced, the ratio of phosphatidylcholines to phosphatidylethanolamines was not affected. In conclusion, this study demonstrates that impaired lipophagy contributes to the pathology of NAFLD and is associated with an altered lipid profile. However, the lipid pattern does not appear to be specific for lipophagic alterations, as it resembles mainly that described in relation to fatty liver disease. |
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| format | Article |
| id | doaj.art-3bb9c4df8f3d4349a59e3db16816a505 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:37:07Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3bb9c4df8f3d4349a59e3db16816a5052023-11-23T20:40:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191186310.3390/ijms231911863Alterations of Lipid Profile in Livers with Impaired LipophagyWenke Jonas0Kristin Schwerbel1Lisa Zellner2Markus Jähnert3Pascal Gottmann4Annette Schürmann5Department of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyNon-alcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation in the liver. Various mechanisms such as an increased uptake in fatty acids or de novo synthesis contribute to the development of steatosis and progression to more severe stages. Furthermore, it has been shown that impaired lipophagy, the degradation of lipids by autophagic processes, contributes to NAFLD. Through an unbiased lipidome analysis of mouse livers in a genetic model of impaired lipophagy, we aimed to determine the resulting alterations in the lipidome. Observed changes overlap with those of the human disease. Overall, the entire lipid content and in particular the triacylglycerol concentration increased under conditions of impaired lipophagy. In addition, we detected a reduction in long-chain polyunsaturated fatty acids (PUFAs) and an increased ratio of n-6 PUFAs to n-3 PUFAs, which was due to the depletion of n-3 PUFAs. Although the abundance of major phospholipid classes was reduced, the ratio of phosphatidylcholines to phosphatidylethanolamines was not affected. In conclusion, this study demonstrates that impaired lipophagy contributes to the pathology of NAFLD and is associated with an altered lipid profile. However, the lipid pattern does not appear to be specific for lipophagic alterations, as it resembles mainly that described in relation to fatty liver disease.https://www.mdpi.com/1422-0067/23/19/11863non-alcoholic fatty liver diseaselipophagylipidomicsfatty acid profilelong-chain polyunsaturated fatty acids |
| spellingShingle | Wenke Jonas Kristin Schwerbel Lisa Zellner Markus Jähnert Pascal Gottmann Annette Schürmann Alterations of Lipid Profile in Livers with Impaired Lipophagy International Journal of Molecular Sciences non-alcoholic fatty liver disease lipophagy lipidomics fatty acid profile long-chain polyunsaturated fatty acids |
| title | Alterations of Lipid Profile in Livers with Impaired Lipophagy |
| title_full | Alterations of Lipid Profile in Livers with Impaired Lipophagy |
| title_fullStr | Alterations of Lipid Profile in Livers with Impaired Lipophagy |
| title_full_unstemmed | Alterations of Lipid Profile in Livers with Impaired Lipophagy |
| title_short | Alterations of Lipid Profile in Livers with Impaired Lipophagy |
| title_sort | alterations of lipid profile in livers with impaired lipophagy |
| topic | non-alcoholic fatty liver disease lipophagy lipidomics fatty acid profile long-chain polyunsaturated fatty acids |
| url | https://www.mdpi.com/1422-0067/23/19/11863 |
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