Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba
Abstract Background Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis, an obligate intracellular Gram-negative bacterium of the family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes for the major immunoreactive...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-022-05426-0 |
| _version_ | 1811188867145924608 |
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| author | Maylin González Navarrete Adnan Hodžić Belkis Corona-González Matheus Dias Cordeiro Claudia Bezerra da Silva Liani Coronado Báez Dasiel Obregón Daniel Moura de Aguiar Amanda Noeli da Silva Campos Ísis Indaiara Gonçalves Granjeiro Taques Alejandra Wu-Chuang Eugenio Roque López Elianne Piloto-Sardiñas Lianet Abuin-Denis Adivaldo Henrique da Fonseca Alejandro Cabezas-Cruz |
| author_facet | Maylin González Navarrete Adnan Hodžić Belkis Corona-González Matheus Dias Cordeiro Claudia Bezerra da Silva Liani Coronado Báez Dasiel Obregón Daniel Moura de Aguiar Amanda Noeli da Silva Campos Ísis Indaiara Gonçalves Granjeiro Taques Alejandra Wu-Chuang Eugenio Roque López Elianne Piloto-Sardiñas Lianet Abuin-Denis Adivaldo Henrique da Fonseca Alejandro Cabezas-Cruz |
| author_sort | Maylin González Navarrete |
| collection | DOAJ |
| description | Abstract Background Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis, an obligate intracellular Gram-negative bacterium of the family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes for the major immunoreactive protein TRP36 in E. canis, has been successfully used to characterize the genetic diversity of this pathogen in different regions of the world. Based on trp36 sequence analysis, four E. canis genogroups, United States (US), Taiwan (TWN), Brazil (BR) and Costa Rica (CR), have been identified. The aim of this study was to characterize the genetic diversity of E. canis in Cuba based on the trp36 gene. Methods Whole blood samples (n = 8) were collected from dogs found to be infested with the tick vector Rhipicephalus sanguineus sensu lato (s.l.) and/or presenting clinical signs and symptoms of CME. Total DNA was extracted from the blood samples and trp36 fragments were amplified by PCR. Nucleotide and protein sequences were compared using alignments and phylogenetic analysis. Results Four of the trp36 sequences obtained (n = 8) fall within the phylogenetic cluster grouping the US genogroup E. canis strains. The other E. canis trp36 sequences formed a separate and well-supported clade (94% bootstrap value) that is phylogenetically distant from the other major groups and thus represents a new genogroup, herein designated as the ‘Cuba (CUB) genogroup’. Notably, dogs infected with the CUB genogroup presented frequent hemorrhagic lesions. Conclusions The results of this study suggest that genetic diversification of E. canis in Cuba is associated with the emergence of E. canis strains with increased virulence. |
| first_indexed | 2024-04-11T14:25:18Z |
| format | Article |
| id | doaj.art-3bbeb734f0df4ceb8f46dbad8764169e |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-11T14:25:18Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-3bbeb734f0df4ceb8f46dbad8764169e2022-12-22T04:18:53ZengBMCParasites & Vectors1756-33052022-08-011511910.1186/s13071-022-05426-0Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in CubaMaylin González Navarrete0Adnan Hodžić1Belkis Corona-González2Matheus Dias Cordeiro3Claudia Bezerra da Silva4Liani Coronado Báez5Dasiel Obregón6Daniel Moura de Aguiar7Amanda Noeli da Silva Campos8Ísis Indaiara Gonçalves Granjeiro Taques9Alejandra Wu-Chuang10Eugenio Roque López11Elianne Piloto-Sardiñas12Lianet Abuin-Denis13Adivaldo Henrique da Fonseca14Alejandro Cabezas-Cruz15Department of Preventive Veterinary Medicine, Agrarian University of HavanaDepartment of Pathobiology, Institute of Parasitology, University of Veterinary Medicine ViennaDirection of Animal Health, National Center for Animal and Plant HealthDepartment of Animal Parasitology, Federal Rural University of Rio de Janeiro (UFRRJ)Department of Animal Parasitology, Federal Rural University of Rio de Janeiro (UFRRJ)Direction of Animal Health, National Center for Animal and Plant HealthSchool of Environmental Sciences, University of GuelphVirology and Rickettsioses Laboratory, Hospital Veterinário da Facultade de Medicina Veterinária, Federal University of Mato Grosso StateVirology and Rickettsioses Laboratory, Hospital Veterinário da Facultade de Medicina Veterinária, Federal University of Mato Grosso StateVirology and Rickettsioses Laboratory, Hospital Veterinário da Facultade de Medicina Veterinária, Federal University of Mato Grosso StateUMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-EstDepartment of Preventive Veterinary Medicine, Agrarian University of HavanaDirection of Animal Health, National Center for Animal and Plant HealthAnimal Biotechnology Department, Center for Genetic Engineering and BiotechnologyDepartment of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro (UFRRJ)UMR BIPAR, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-EstAbstract Background Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis, an obligate intracellular Gram-negative bacterium of the family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes for the major immunoreactive protein TRP36 in E. canis, has been successfully used to characterize the genetic diversity of this pathogen in different regions of the world. Based on trp36 sequence analysis, four E. canis genogroups, United States (US), Taiwan (TWN), Brazil (BR) and Costa Rica (CR), have been identified. The aim of this study was to characterize the genetic diversity of E. canis in Cuba based on the trp36 gene. Methods Whole blood samples (n = 8) were collected from dogs found to be infested with the tick vector Rhipicephalus sanguineus sensu lato (s.l.) and/or presenting clinical signs and symptoms of CME. Total DNA was extracted from the blood samples and trp36 fragments were amplified by PCR. Nucleotide and protein sequences were compared using alignments and phylogenetic analysis. Results Four of the trp36 sequences obtained (n = 8) fall within the phylogenetic cluster grouping the US genogroup E. canis strains. The other E. canis trp36 sequences formed a separate and well-supported clade (94% bootstrap value) that is phylogenetically distant from the other major groups and thus represents a new genogroup, herein designated as the ‘Cuba (CUB) genogroup’. Notably, dogs infected with the CUB genogroup presented frequent hemorrhagic lesions. Conclusions The results of this study suggest that genetic diversification of E. canis in Cuba is associated with the emergence of E. canis strains with increased virulence.https://doi.org/10.1186/s13071-022-05426-0TicksEhrlichia canisCanine monocytic ehrlichiosisDogstrp36Rhipicephalus sanguineus |
| spellingShingle | Maylin González Navarrete Adnan Hodžić Belkis Corona-González Matheus Dias Cordeiro Claudia Bezerra da Silva Liani Coronado Báez Dasiel Obregón Daniel Moura de Aguiar Amanda Noeli da Silva Campos Ísis Indaiara Gonçalves Granjeiro Taques Alejandra Wu-Chuang Eugenio Roque López Elianne Piloto-Sardiñas Lianet Abuin-Denis Adivaldo Henrique da Fonseca Alejandro Cabezas-Cruz Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba Parasites & Vectors Ticks Ehrlichia canis Canine monocytic ehrlichiosis Dogs trp36 Rhipicephalus sanguineus |
| title | Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba |
| title_full | Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba |
| title_fullStr | Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba |
| title_full_unstemmed | Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba |
| title_short | Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba |
| title_sort | novel ehrlichia canis genogroup in dogs with canine ehrlichiosis in cuba |
| topic | Ticks Ehrlichia canis Canine monocytic ehrlichiosis Dogs trp36 Rhipicephalus sanguineus |
| url | https://doi.org/10.1186/s13071-022-05426-0 |
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